Epicoccum jingdongense Y. Wang, Y. Tu, X. Chen, H. Jiang, H. Ren, Q. Lu, C. Wei & W. Lv, 2024

Wang, Yuchun, Tu, Yiyi, Chen, Xueling, Jiang, Hong, Ren, Hengze, Lu, Qinhua, Wei, Chaoling & Lv, Wuyun, 2024, Didymellaceae species associated with tea plant (Camellia sinensis) in China, MycoKeys 105, pp. 217-251 : 217-251

Epicoccum jingdongense Y. Wang, Y. Tu, X. Chen, H. Jiang, H. Ren, Q. Lu, C. Wei & W. Lv sp. nov.

Fig. 7

Etymology.

Named after the location where it was collected, Jingdong Yizu Autonomous County.

Description.

Sexual morph: undetermined. Asexual morph: Pycnidia smooth, subglobose, pale brown. Conidia ellipsoidal to subcylindrical, pale yellow, smooth, generated from pycnidia, aseptate, 7.1–16 × 4–9 µm (av. = 10.7 ± 1.2 × 5.4 ± 0.6 µm, n = 30). Mycelia extensively branched from subapical hyphal compartments, septate, hyaline.

Culture characteristics.

Colonies on PDA reaching 35–42 mm diam. after 7 days at 28 ° C in the dark, margin irregular, aerial mycelium flat, pale brown to rosy, white edges; reverse black to brown, pale buff edges. Pycnidia and conidia produced on the colony surface after being cultured for 14 days at 28 ° C in the dark. Colonies on OA reaching 49–55 mm diam. after 7 days at 28 ° C in the dark, margin regular, aerial mycelium flat, pale buff to whitish; reverse concolorous.

Materials examined.

China, Yunnan Province, Puer City, Jingdong Yizu Autonomous County, from healthy leaves of C. sinensis , 13 Jun 2020, Y. C. Wang, Holotype HMAS 352389 , culture ex-type CGMCC 3.24247 = YCW 1868 . Yunnan Province, Puer City, Jingdong Yizu Autonomous County, from healthy leaves of C. sinensis , 13 Jun 2020, Y. C. Wang, culture ex-type CGMCC 3.24248 = YCW 1937 .

Notes.

Epicoccum jingdongense is closely related to E. dendrobii and E. puerense with high statistical support (83 % / 0.99, ML / PP, Fig. 3 ). Epicoccum puerense differs in 1 bp in ITS and 40 bp in TUB 2 from E. dendrobii . The conidia size is larger than that of E. dendrobii . In the present study, six strains were isolated from healthy tea plant leaves. It was isolated and identified from tea plant for the first time.

Figures

Figure 3. Phylogenetic tree generated by Maximum Likelihood analysis, based on the combined ITS, LSU, RPB 2 and TUB 2 dataset of Epicoccum species. Bootstrap support values above 50 % and Bayesian posterior values above 0.75 are shown at each node (ML / PP). Pleiochaeta setosa CBS 118.25 / CBS 496.63 and Co. palmarum CBS 400.71 are used as outgroups. Ex-type strains are emphasised in bold.

Figure 7. Epicoccum jingdongense YCW 1868 A, B colony on PDA (front and reverse) C, D colony on OA (front and reverse) E mycelia F pycnidia forming on PDA G conidia. Scale bars: 10 μm (E – G).