Apiospora dongyingensis R.Y. Liu, J.W. Xia & X.G. Zhang, 2023

Liu, Rongyu, Li, Duhua, Zhang, Zhaoxue, Liu, Shubin, Liu, Xinye, Wang, Yixin, Zhao, Heng, Liu, Xiaoyong, Zhang, Xiuguo, Xia, Jiwen & Wang, Yujiao, 2023, Morphological and phylogenetic analyses reveal two new species and a new record of Apiospora (Amphisphaeriales, Apiosporaceae) in China, MycoKeys 95, pp. 27-45 : 27

Apiospora dongyingensis R.Y. Liu, J.W. Xia & X.G. Zhang sp. nov.

Fig. 2

Etymology.

Named after Dongying City (China) where the type was collected.

Type.

China, Shandong Province: Dongying Botanical Garden , on diseased leaves of bamboo, 13 July 2022, R.Y. Liu, holotype HSAUP 0302, ex-type living culture SAUCC 0302 .

Description.

Asexual morph: On WA, hyphae 1.3-3.6 μm diam., hyaline, branched, septate. Conidiophores cylindrical, septate, verrucose, flexuous, sometimes reduced to conidiogenous cells. Conidiogenous cells globose to subglobose, erect, blastic, aggregated in clusters on hyphae, hyaline to pale brown, smooth, branched, 8.2-13.9 × 4.2-8.2 μm, mean ± SD: 9.6 ± 1.6 × 6.7 ± 1.1 μm (n = 40). Conidia globose, subglobose to lenticular, with a longitudinal germ slit, occasionally elongated to ellipsoidal, brown to dark brown, smooth to finely roughened, 8.0-16.5 × 5.5-9.0 μm, mean ± SD: 9.4 ± 1.9 × 7.3 ± 1.0 μm, L/W = 1.3-1.9 (n = 40). Sexual morph: Undetermined.

Culture characteristics.

Colonies on PDA flat with entire margin, aerial mycelium white to gray, floccose cottony; surface and reverse gray in the center and grayish margin. PDA attaining 78.5-86.5 mm in diameter after 7 days at 25 °C, growth rate 11.0-12.5 mm/day.

Additional specimen examined.

China, Shandong Province: Dongying Botanical Garden, on diseased leaves of bamboo, 13 July 2022, R.Y. Liu, paratype HSAUP 0303, ex-paratype living culture SAUCC 0303.

Notes.

Apiospora dongyingensis is closely related but phylogenetically distinct from A. camelliae-sinensis (M. Wang, F. Liu & L. Cai) Pintos & P. Alvarado and A. cyclobalanopsidis (Y. Feng & Jian K. Liu) X.G. Tian & Tibpromma (Fig. 1 ). A. dongyingensis differs from A. camelliae-sinensis by 18 nucleotides (13/518 in ITS, 2/804 in LSU, 2/374 in tef1 and 1/265 in tub2) and A. cyclobalanopsidis by 58 nucleotides (17/518 in ITS, 4/799 in LSU, 26/377 in tef1 and 11/266 in tub2). Morphologically, it differs from A. camelliae-sinensis and A. cyclobalanopsidis in its conidia (globose, subglobose to lenticular, 8.0-16.5 × 5.5-9.0 μm in A. dongyingensis vs. globose to subglobose, 9.0-13.5 × 7.0-12.0 μm in A. camelliae-sinensis and surface view globose to ellipsoid, 8-12 μm long and side view lenticular, 10-14 μm long in A. cyclobalanopsidis ; Wang et al. 2018; Feng et al. 2021; Pintos and Alvarado 2021; Tian et al. 2021).

Figures

Figure 1. Phylogram of Apiospora based on combined ITS, LSU, tef 1 and tub 2 genes. ML bootstrap support values (MLBS ≥ 50 %) and Bayesian posterior probability (BYPP ≥ 0.90) are shown as first and second position above nodes, respectively. Strains from this study are shown in red, ex-type or ex-epitype cultures are indicated in bold face. Some branches were shortened according to the indicated mulipliers.

Figure 2. Apiospora dongyingensis (SAUCC 0302, ex-holotype culture) a leaf of host plant b, c surface (b) and reverse (c) sides of colony after incubation for 7 days on PDA d conidiomata formed in culture e, f conidiogenous cells and conidia g, h conidia. Scale bars: 10 μm (e-h).