Sporothrix undulata R. Jankowiak & A. Ostafinska, 2021

Ostafinska, Agnieszka, Jankowiak, Robert, Bilanski, Piotr, Solheim, Halvor & Wingfield, Michael J., 2021, Six new species of Sporothrix from hardwood trees in Poland, MycoKeys 82, pp. 1-32 : 1

Sporothrix undulata R. Jankowiak & A. Ostafinska sp. nov.

Fig. 10

Etymology.

Referring to the aerial mycelium growing in undulating concentric zones on MEA.

Type.

Poland, Małopolskie Province, Wierzchosławice, from Epuraea guttata on Quercus robur , June 2016, R. Jankowiak, (O-F- 258635 holotype, culture ex-type CBS 147929) .

Description.

Sexual and asexual structures produced on sterilised oak twigs and on surface of malt agar in Petri dishes. Ascomata abundant, superficially or partly embedded in the agar, single or in groups; ascomatal base black, globose, (65-)95-186(-223) μm diam., with brown hyphal hairs, 8 to 134 μm long and 1.2 to 3.1 μm wide at the base; ascomatal necks black, straight or curved, (114-)174-482(-697) μm long, diameter (9.1-)12.3-18.7(-24.2) μm at the apex and (14.7-)22-40.3(-58.7) μm at the base. Ostiolar hyphae present, pale brown, with small granules, septate, straight or slightly waved, tapering towards the apex or sporadically dichotomously branched at the tip, (9-)16-28(-31) in number, (29.4-)39.9-59.5(-72) μm long, (0.4-)0.6-1(-1.1) μm at the apex and (1.5-)1.8-2.7-(3.3) μm at the base. Asci subglobose to ovoid, (5.7-)6.7-8.5(-9.4) × (3.4-)4.4-5.8(-6.4) μm. Ascospores one-celled, kidney-shaped to almost triangular in side view (3.4-)3.8-4.6(-4.9) × (1.1-)1.4-1.7(-2) μm, elliptical in front view (3.2-)3.5-4.5(-5.6) × (0.9-)1.5-2.1(-2.8) μm, sometimes with residual sheath up to 0.6 μm thick, accumulated in white-colored mass at the tip of the neck. Conidiophores hyaline, micronematous or semimacronematous, simple or occasionally branched and bearing several conidiogenous cells, either borne on vegetative hyphae or on upright hyphae. Conidiogenous cells blastic, cylindrical, terminal, lateral or intercalary, straight or curved, slightly tapering towards the apex, swollen apical part forming conidia by sympodial proliferation on small or hardly visible denticles, (5.2-)11.3-50.4(-112.2) μm long, (0.9-)1.3-1.8(-2.1) μm wide at the base. Apical part (1.1-)1.6-3.4(-5.9) μm long and (1.1-)1.7-3.5(-5.4) μm wide. Conidia of two types: 1) sparsely in cultures, hyaline, unicellular, smooth, ellipsoid, pointed at the base, (3.2-)4.2-7.8(-11.7) × (1.4-)1.7-2.4(-3.5) μm, formed directly on denticles; 2) abundant in cultures, subhyaline to lightly pigmented, unicellular, smooth, subglobose to globose, sometimes pointed at the base, (2.1-)2.9-4.2(-5.5) μm diam, formed singly or in chains, either directly on the side of vegetative hyphae, on short lateral branches or denticles. Culture characteristics: Cultures showing optimum growth at 25 °C (1.2 mm/d) with growth somewhat slower at 20 °C and 30 °C (0.9 mm/d), white or white grey, flat, growing in a circular pattern with undulate margins.

Host tree.

Alnus glutinosa , Carpinus betulus , Fagus sylvatica , Quercus robur , Quercus rubra , Salix fragilis .

Insect vector.

Cryptarcha undata , Epuraea guttata .

Distribution.

Poland.

Additional specimen examined.

Poland, Małopolskie Province, Wierzchosławice, from wound on Quercus robur , October 2015, R. Jankowiak (O-F-258636, cultures CBS 147931).

Notes.

This species is phylogenetically distinct from the other Sporothrix species based on the ITS, βT, CAL and TEF1-α sequences. Sporothrix undulata is phylogenetically closely related to S. cryptarchum described in this study. The morphological differences between S. undulata and S. cryptarchum are described in the section above treating S. cryptarchum .

Sporothrix undulata was represented by nine isolates collected from Poland. It corresponds to Sporothrix sp. 12 in the study of Jankowiak et al. (2019b). In this study S. undulata was isolated from wounds on hardwood trees and from adults of nitidulid beetles ( Coleoptera : Nitidulidae ), which visited wounds on Quercus robur .

Figures

Figure 10. Sporothrix undulata sp. nov. (CBS 147929) a ascoma b ascomatal base c ostiolar hyphae d asci e ascospores f-h globose conidia arising on long conidiophore or directly from hyphae i globose conidia j conidiogenous cell with an inflated cluster of denticles at the apex k conidia l-m fourteen-day-old culture on MEA (left- pigmented CBS 147929, right - white KFL 404 DB 16 bRJCU). Scale bars: 100 μm (a), 25 μm (b-d), 10 μm (e), 25 μm (f), 10 μm (g, h), 5 μm (i), 10 μm (j), 5 μm (k).