Sugiyamaella yunanensis C.Y. Chai & F.L. Hui, 2021

Shi, Cheng-Feng, Zhang, Kai-Hong, Chai, Chun-Yue, Yan, Zhen-Li & Hui, Feng-Li, 2021, Diversity of the genus Sugiyamaella and description of two new species from rotting wood in China, MycoKeys 77, pp. 27-39 : 27

Sugiyamaella yunanensis C.Y. Chai & F.L. Hui sp. nov. Figure 2

Type.

China, Yunnan Province, Jinghong City, Mengyang Town, in rotting wood from a tropical rainforest, July 2016, K.F. Liu & L. Zhang (holotype NYNU 161059T, culture ex-type CBS 14701).

Etymology.

The species name yunanensis (N.L. fem. adj.) refers to the geographical origin of the type strain of this species.

Description.

The cells are ovoid to elongate (2.5-5.5 × 3-7.5 μm) and occur singly or in pairs after being placed in YM broth for 3 days at 25 °C (Fig. 2A ). Budding is multilateral. After 3 days of growth on YM agar at 25 °C, the colonies are white to cream-coloured, buttery and smooth, with entire margins. After 7 days at 25 °C on a Dalmau plate culture with CM agar, hyphae and blastoconidia are formed (Fig. 2B ). Asci or signs of conjugation were not observed on sporulation media. Glucose and d-xylose are weakly fermented. Glucose, galactose, l-sorbose, d-glucosamine, d-xylose, l-arabinose, d-arabinose, sucrose, maltose, trehalose, methyl α-d-glucoside, cellobiose, salicin, arbutin, melibiose, raffinose, inulin, ribitol, d-glucitol, d-mannitol, d-glucono-1, 5-lactone, 2-keto-d-gluconate, d-gluconate, d-glucuronate, dl-lactate, succinate, citrate and ethanol are assimilated. No growth was observed in d-ribose, l-rhamnose, lactose, melezitose, glycerol, erythritol, xylitol, galactitol, myo-inositol or methanol. In nitrogen-assimilation tests, growth is present on nitrate, nitrite, l-lysine and glucosamine, while growth is absent on ethylamine, cadaverine, creatine, creatinine, imidazole and d-tryptophan. Growth is observed at 37 °C, but not at 40 °C. Growth in the presence of 0.01% cycloheximide is present, but growth in the presence of 10% sodium chloride (NaCl) with 5% glucose and 1% acetic acid is absent. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are negative.

Additional isolate examined.

China, Yunnan Province, Jinghong City, Mengyang Town, in rotting wood from a tropical rainforest, July 2016, K.F. Liu & L. Zhang, NYNU 16113.

GenBank accession numbers.

holotype NYNU 161059T (ITS: MT257259; nrLSU D1/D2: MT257257); additional isolate NYNU 16113 (ITS: MT257256; nrLSU D1/D2: MT257261).

Notes.

Two isolates, representing S. yunanensis , are retrieved in a well-supported clade and appear most closely related to S. valdiviana (Fig. 1 ). Sugiyamaella yunanensis can be distinguished from S. valdiviana , based on ITS and nrLSU D1/D2 loci (6/510 in ITS and 7/557 in nrLSU D1/D2). Physiologically, S. yunanensis differs from S. valdiviana by its ability to assimilate inulin and dl-lactate and its inability to assimilate melezitose, glycerol and myo-inositol. Additionally, S. valdiviana grows in the presence of 0.1% cycloheximide, while S. yunanensis does not ( Kurtzman 2007).

Figures

Figure 1. Maximum Likelihood phylogenetic tree of Sugiyamaella inferred from the combined ITS and nrLSU dataset and rooted with Schizosaccharomyces pombe NRRL Y- 12796. The ML and MP bootstrap support values above 50 % are shown at the first and second positions, respectively. Newly-sequenced collections are in black boldface.

Figure 2. Morphology of S. yunanensis A budding cells after 3 days in YM broth at 25 ° C B hyphae and blastoconidia on corn-meal agar after 7 days at 25 ° C. Scale bars: 10 μm.