Pseudotetraploa yunnanensis X. Tang, Jayaward., R. Jeewon & J.C. Kang, 2023

Tang, Xia, Jeewon, Rajesh, Lu, Yong-Zhong, Alrefaei, Abdulwahed Fahad, Jayawardena, Ruvishika S., Xu, Rong-Ju, Ma, Jian, Chen, Xue-Mei & Kang, Ji-Chuan, 2023, Morphophylogenetic evidence reveals four new fungal species within Tetraplosphaeriaceae (Pleosporales, Ascomycota) from tropical and subtropical forest in China, MycoKeys 100, pp. 171-204 : 171

Pseudotetraploa yunnanensis X. Tang, Jayaward., R. Jeewon & J.C. Kang sp. nov.

Fig. 5

Etymology.

The specific epithet ' yunnanensis ' refers to the place where the fungus was collected, Yunnan Province, China.

Holotype.

HKAS 129442.

Description.

Saprobic on bamboo. Teleomorph not observed. Anamorph Hyphomycetous. Colonies effuse, gregarious on host substrate, brown to dark brown. Mycelium superficial, hyaline to pale brown. Conidiophores absent. Conidiogenous cells micronematous, mononematous, monoblastic, integrated, usually undistinguishable from superficial hyphae. Conidia 67-120 × 16.5-35 μm (x̅ = 95 × 24 μm, n = 20), solitary, septate, brown to dark brown, ovoid to obclavate or narrowly obpyriform, consisting of 3-6 columns of cells, rounded at the base 19-36 μm wide (x̅ = 26 μm, n = 20), slightly constricted at septa, rarely branched and make V-shaped conidia; setose appendages at the apical part 15-87 × 3.5-7 μm (x̅ = 37 × 5 μm, n = 20), appendages 3-6 in number, 1-8-septate, brown at the base and almost hyaline at the apex, smooth, unbranched, shorter appendage is straight and longer appendage is curved.

Culture characteristics.

Conidia germinated from both ends on PDA and incubated at room temperature (25 ˚C). Colonies circular, cottony, flat, slightly grey with an entire margin, containing a circular white mycelium in the centre. The reverse side is a pale brown in the centre that gradually extends outwards while the colour changes to pale grey, with a brown margin and no pigment.

Material examined.

China, Yunnan Province, Puer City, Ailao mountains , on bamboo, May 23, 2022, Rong-Ju Xu, ALS 29 (HKAS 129442, holotype), ex-type culture KUNCC 10464 .

Notes.

Pseudotetraploa yunnanensis is similar to Ps. curviappendiculata and Ps. longissima . However, Pseudotetraploa yunnanensis differs from Ps. curviappendiculata in having branched and V-shaped conidia, consisting of 3-6 columns of cells with 3-6 apical appendages, larger conidia [67-120 μm vs. 52-67(-75) μm] in length and [16-35 μm vs. 15-22 μm] in width, while Ps. curviappendiculata consists of 4-5 columns of cells with 4 apical appendages; Pseudotetraploa yunnanensis differs from Ps. longissima in having smaller conidia [67-120 μm vs. (98-)110-148(-155) μm] in length and [16-35 μm vs. 18-25 μm] in width, without verrucose at the base. The phylogenetic analysis showed that Pseudotetraploa yunnanensis is sister to Ps. rajmachiensis and Ps. javanica . The comparison of pairwise nucleotides showed that Pseudotetraploa yunnanensis is different from Ps. rajmachiensis in 27/1021 bp (2.6%) in LSU and 30/560 (6%) in ITS; Pseudotetraploa yunnanensis is different from Ps. javanica in 11/1020 bp (1.1%) in LSU and 17/538 (3.2%) in ITS. Thus, we describe Pseudotetraploa yunnanensis herein as a novel species in Pseudotetraploa according to the guidelines Jeewon and Hyde (2016) and Chethana et al. (2021).

Figures

Figure 5. Pseudotetraploa yunnanensis (HKAS 129442, holotype) a, b colonies on natural substrates c-n conidia. Scale bars: 20 μm (c); 50 μm (d-n).