Yamadazyma luoyangensis C.Y. Chai & F.L. Hui, 2021

Gao, Wan-Li, Li, Ying, Chai, Chun-Yue, Yan, Zhen-Li & Hui, Feng-Li, 2021, New species of Yamadazyma from rotting wood in China, MycoKeys 83, pp. 69-84 : 69

Yamadazyma luoyangensis C.Y. Chai & F.L. Hui sp. nov.

Figure 2

Type.

China, Henan Province, Luoyang City , Song County, in rotting wood from a forest park, September 2020, J.Z. Li & Z.T Zhang (holotype NYNU 201023T, culture ex-type CBS 16666 , CICC 33509) .

Etymology.

The species name Yamadazyma luoyangensis refers to the geographical origin of the type strain of this species.

Description.

The cells are ovoid to ellipsoid (2-4 × 3.5-7 μm) and occur singly or in pairs after being placed in YM broth for three days at 25 °C (Figure 2A ). Budding is multilateral. After three days of growth on YM agar at 25 °C, the colonies are white to cream-colored, buttery, and smooth, with entire margins. After seven days at 25 °C on a Dalmau plate culture with CM agar, pseudohyphae are formed, but true hyphae are not (Figure 2B ). Asci or signs of conjugation are not observed on sporulation media. Glucose, galactose, trehalose, and cellobiose are fermented, but maltose, sucrose, melibiose, lactose, melezitose, raffinose, d-xylose, and inulin are not. Glucose, galactose, d-glucosamine, d-ribose, d-xylose, l-arabinose, d-arabinose, l-rhamnose, sucrose, maltose, trehalose, methyl α-d-glucoside, cellobiose, salicin, arbutin, melezitose, inulin, glycerol, erythritol, ribitol, d-glucitol, d-mannitol, galactitol, d-glucono-1, 5-lactone, 5-keto-d-gluconate, d-gluconate, succinate, citrate, and ethanol are assimilated. No growth is observed in l-sorbose, melibiose, lactose, raffinose, myo -inositol, 2-keto-d-gluconate, d-glucuronate, dl-lactate, or methanol. In nitrogen-assimilation tests, growth is present on ethylamine, l-lysine, glucosamine, and d-tryptophan, while growth is absent on nitrate, nitrite, cadaverine, creatine, creatinine, and imidazole. Growth is observed at 35 °C but not at 37 °C. Growth in the presence of 10% NaCl with 5% glucose is present, but growth in the presence of 0.01% cycloheximide and 1% acetic acid is absent. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are negative.

Additional isolate examined.

China, Henan Province, Luoyang City, Song County, in rotting wood from a forest park, September 2020, J.Z. Li & Z.T Zhang, NYNU 201035.

GenBank accession numbers.

Holotype NYNU 201023T (ITS: MW365549; D1/D2 LSU: MW365545); additional isolate NYNU 201035 (ITS: MZ318445; D1/D2 LSU: MZ318422).

Notes.

Two isolates representing Y. luoyangensis were resolved in a well-supported clade and are most closely related to Y. mexicana (Figure 1 ). Yamadazyma luoyangensis can be distinguished from Y. mexicana based on ITS and D1/D2 LSU loci (4/592 in ITS and 10/531 in D1/D2 LSU). Physiologically, Y. luoyangensis differs from Y. mexicana by its ability to assimilate inulin and 5-keto-d-gluconate and its inability to assimilate lactose, raffinose, and 2-keto-d-gluconate. Additionally, Y. mexicana grows at 37 °C, while Y. luoyangensis does not (Table 2 ) ( Kurtzman 2011).

Figures

Figure 1. Maximum likelihood phylogenetic tree of Yamadazyma inferred from the combined ITS and D 1 / D 2 LSU dataset and rooted with Scheffersomyces coipomoensis (CBS 8178) and Babjeviella inositovora (CBS 8006). ML and MP bootstrap support values above 50 % are respectively shown at the first and second positions. Newly sequenced collections are in blue boldface.

Figure 2. Yamadazyma luoyangensis (NYNU 201023, holotype) A budding cells after three days in YM broth at 25 ° C B pseudohyphae on cornmeal agar after seven days at 25 ° C. Scale bars: 10 μm.