Pestalotiopsis trachycarpicola Yan M. Zhang & K.D. Hyde, 2012

Wang, Yifeng, Tsui, Kin-Ming, Chen, Shimei & You, Chongjuan, 2024, Diversity, pathogenicity and two new species of pestalotioid fungi (Amphisphaeriales) associated with Chinese Yew in Guangxi, China, MycoKeys 102, pp. 201-224 : 201

Pestalotiopsis trachycarpicola Yan M. Zhang & K.D. Hyde, 2012

Fig. 4

Conidiogenesis.

Conidiophores reduced to conidiogenous cells, indistinct. Conidiogenous cells were discrete, ampulliform, thin-walled, hyaline, smooth. Conidia fusiform to clavate, straight or slightly curved, olivaceous to brown, 4-septate, 18.5-25 × 4-6 μm, with apical and basal appendages. Basal cell obconic, hyaline, thin-walled, smooth, 3-5 µm; the three median cells dolioform, versicolor, pale brown to brown with septa darker than the rest of the cells, 11.5-13.5 µm, the second cell from base 3.5-6 µm; the third cell 3.5-4.5 µm; the fourth cell 3.5-5 µm; apical cell 2.5-4 µm, cylindrical, hyaline; 2-4 tubular apical appendages, arising from the apex of the apical cell each at different point, filiform, 5-15 µm; basal appendage present most of the time, single, tubular, unbranched, 3.5-4.5 µm (Fig. 4c-e ). Sexual morph not observed.

Culture characteristics.

Colonies on PDA reaching 90 mm diameter after seven days at 25 °C, with an undulate and radial edge, with dense aerial mycelium on surface, white to faint yellow on front, pale honey-coloured on the reverse side (Fig. 4a ). Conidiomata acervular in culture on PDA, globose, 100-500 μm in diameter, solitary or aggregated in clusters, exuding black conidial masses (Fig. 4b ).

Material examined.

China, Guangxi Province, from diseased needles of Chinese yew, May 2020, Y. F. Wang (BJFC-S1955); living cultures BJFUCC42, BJFUCC42-2 .

Notes.

Pestalotiopsis trachycarpicola was originally described from leaves of Trachycarpus fortunei in Kunming Botany Garden, Kunming, Yunnan Province, China ( Zhang et al. 2012). In the present study, the two isolates clustered with P. trachycarpicola and P. kenyana with high support values (MP/ML = 86/100). P. kenyana (CBS 442.67, ex-type) and P. trachicarpicola (OP068, ex-type MFLUCC 12-0263) were demonstrated to be the same species, as there was no genetic distance between the two samples (Suppl. material 3). Morphologically, our new collections resembled P. trachycarpicola in colour and size of the conidiogenous cells, conidia and appendages ( Zhang et al. 2012). Therefore, we reported the two isolates as a new host record of P. trachycarpicola from yews.

Figures

Figure 4. Pestalotiopsis trachycarpicola (BJFUCC 42) a culture on PDA b conidiomata formed on PDA c-e conidia. Scale bars: 500 µm (b); 10 µm (c-e).