Antispila uenoi Kuroko, 1987

Wang, Nan, Liu, Tengteng, Xu, Jiasheng & Jiang, Bin, 2018, The leaf-mining genus Antispila Huebner, 1825 feeding on Vitaceae in Shandong Peninsula, China with one new species (Lepidoptera, Heliozelidae), ZooKeys 744, pp. 49-65 : 53-57

Antispila uenoi Kuroko, 1987 Figs 3-5, 7, 8, 10, 16-18, 19-25, 30-31, 39-41, 46-49

Antispila uenoi Kuroko, 1987: 113. TL: Japan (Iwate Prefecture). TD: UOP.

Material examined.

China: 2♂, 3♀, Mt. Laoshan, Qingdao, Shandong Province, 120.609°E, 36.204°N, 400 m, larva coll. 2017.vii.01, mine on leaf serration of Vitis amurensis , case made vii.03, emerged vii.15, collectors Tengteng Liu and Nan Wang, genitalia no. SDNU.LIU0008♀, SDNU.LIU0015♂, DNA voucher slide no. SDNU.LIU0011♂ (whole body on one slide), registered no. SDNU.QD170705.1-3; 2♂, 2♀, Mt. Laoshan, Qingdao, Shandong Province, 120.609°E, 36.204°N, 400 m, larva coll. 2017.vii.01, mine on leaf basal area, case made vii.03, emerged vii.15, genitalia no. SDNU.LIU0043♀, DNA voucher slide no. SDNU.LIU0012♂ (whole body on one slide), collectors Tengteng Liu and Zhenquan Gao, registered nos. SDNU.QD170707, SDNU.QD1707.1-2.

Diagnosis.

Kuroko (1987) gave a detailed diagnosis to distinguish A. uenoi from A. ampelopsia .

Description.

Adult (Figs 3-5, 7). Forewing length 1.6-1.8 mm. Head silvery gray, with reddish and purple reflection. Antennae dark fuscous, silvery on distal two segments. Labial palpus silvery gray, pointed apically. Thorax and tegula dark fuscous. Legs black, with whitish gray pigmentation on distal part of tarsomeres. Forewing blackish fuscous, with strong purple reflection; an oblique silvery fascia from before middle on costa to basal 1/4 on dorsum, a triangular silvery spot on costal 3/4, with a similar one opposite to it near tornus; cilia unicolorous with forewing on basal 3/4, whitish gray on distal 1/4. Hind wing gray, cilia darker. Abdomen dark gray dorsally, gray ventrally.

Female with forewing patterns more distinct (Figs 4, 5).

Venation (Fig. 10). Forewing with Sc reaching before middle on costa; R1 from 2/5 on upper margin of cell to costal 3/5, Rs1 from distal 1/7 on upper margin of cell to costal 3/4, Rs2 from well beyond distal end of cell, Rs3+4 reaching costa before apex; cell truncated distally; M1 stalked with Rs3+4, to termen near apex, M2+3 from lower corner of distal end of cell; CuA from distal 1/7 of lower margin of cell; A1+2 to beyond middle of dorsum. Hindwing with Sc to beyond middle of costa, R+M ending in 4 branches: Rs to costa, M1 to dorsum near apex, M2 and M3 to dorsum; Cu to middle of dorsum; A1+2 weak. Male with one long frenulum, female bearing two shorter frenular bristles.

Male genitalia (Figs 16-25). Tuba analis developed. Uncus bar-shaped, with two papillae bearing two short setae each at middle, bearing one long and a few shorter setae laterally (Fig. 22). Vinculum shorter than phallus, rounded on anterior margin. Valva semicircular on ventral margin, digital process about half the width of valva, pecten with 12 comb teeth (Figs 20, 21). Juxta longer than half length of phallus, anterior arrow broad and almost semicircular. Phallus as long as length of vinculum + tegumen, narrowed anteriorly; phallotheca with groups of spines, more concentrated and larger ventrally (Figs 24, 25); distal part with two processes ventrally, one large and curved, the other V-shaped with one branch larger than the other, two smaller similar processes dorso-apically, one less sclerotized and curved process at apex with several membranous teeth ventrally (Fig. 23). Paired tufts of slender scales on the 7th abdominal segment (Figs 8, 17, 18).

Female genitalia (Figs 30-32). Ovipositor with six cusps at either side, with apical three smaller, tip distinctly indented (Fig. 32). Vestibulum membranous, with a sclerotized granule and a weak circular ring surrounding opening of canalis spiralis (Fig. 30). Corpus bursae membranous.

DNA barcode.

Two DNA barcodes were obtained (Fig. 33). A partial DNA barcode of 268 bp generated from a paratype of A. uenoi (RMNH.INS.24531) was used for identification of the Chinese specimens. The genetic distance between the Chinese specimens and the paratype is 1.53%.

Host plants.

Vitis amurensis Rupr. (Fig. 39), V. coignetiae Pulliat ex Planch. and V. labruscana L.H. Bailey ( Kuroko 1987). Vitis amurensis is recorded here as a new host.

Biology.

Leaf-mines on Vitis amurensis can occupy serrations along the leaf margin (Figs 40, 41, 46-48) or the leaf basal area (Fig. 49) in an almost equal proportion, calculated from our rearing data (5: 4); no other placements (e. g. leaf central area) of mines were observed, although in Japan the majority of mines occupy the apical or marginal area of the leaves on other hosts ( Kuroko 1987). Frass often dispersed along mines (Figs 47-49). This species overwinters as a prepupa in the shield. Two generations probably occur in Shandong Peninsula.

Distribution.

China (Shandong), Japan: Honshu. The host plant Vitis amurensis is widespread in the northeast and eastern parts of China ( Chen et al. 2007), Eastern Russia ( Afonin et al. 2008), Japan: Honshu and Korea ( Ohwi 1965). A much wider distribution of the moth is expected, where its host plants occur.

Remarks.

This species is newly recorded in China. The venation and the paired tufts of scales on the 7th abdominal segment in male are illustrated for A. uenoi for the first time.