Trypanosoma spp

Rodrigues, Marina Silva, Lima, Luciana, Xavier, Samanta Cristina das Chagas, Herrera, Heitor Miraglia, Rocha, Fabiana Lopes, Roque, André Luiz Rodrigues, Teixeira, Marta Maria Geraldes & Jansen, Ana Maria, 2019, Uncovering Trypanosoma spp. diversity of wild mammals by the use of DNA from blood clots, International Journal for Parasitology: Parasites and Wildlife 8, pp. 171-181 : 174

3.2. Diversity of Trypanosoma spp . detected by PCR of blood clots

Thirteen Trypanosoma species or MOTUs were identified, among them, two new MOTUs ( T. sp. Neobat 4 and T. sp. DID). All main branches had high support (> 85) for at least two methods of phylogenetic tree reconstruction ( Figs. 3 and 4). PCR from blood clots showed that Didelphimorphia presented a higher infection rate (88.0%) in comparison with Chiroptera (66.7%) and Carnivora (60.0%) and also demonstrated to harbor the highest diversity of trypanosome species ( Table 1; Fig. 2). Didelphis spp . was the taxon that displayed the highest Trypanosoma spp . diversity ( Fig. 2). T. cruzi was the species found infecting the highest number of individuals (marsupials, carnivores and bats), followed by T. lainsoni (marsupials and bats), T. dionisii (marsupials, bats and carnivores) ( Table 1; Fig. 2). In bats, we observed a new MOTU that we labeled as Trypanosoma sp. Neobat 4; further on, Trypanosoma sp. Neobat 2 and Neobat 3 ( Fig. 3). Concerning marsupials, infections by a new MOTU that we named DID, besides infections by T. cascavelli , Trypanosoma janseni , Trypanosoma rangeli A, and sequences closely related to Trypanosoma gennarii have been observed ( Fig. 4).