Cortinarius laccariphyllus Y. Li & M.L. Xie, 2020

Xie, Meng-Le, Wei, Tie-Zheng, Fu, Yong-Ping, Li, Dan, Qi, Liang-Liang, Xing, Peng-Jie, Cheng, Guo-Hui, Ji, Rui-Qing & Li, Yu, 2020, Three new species of Cortinarius subgenus Telamonia (Cortinariaceae, Agaricales) from China, MycoKeys 69, pp. 91-109 : 91

Cortinarius laccariphyllus Y. Li & M.L. Xie sp. nov. Figures 2a, b , 3a , 4a

Diagnosis.

Pileus 2.2-6.6 cm in diam., strongly hygrophanous, translucently striate. Lamellae distant, Laccaria -like when young. Universal veil white, extremely sparse. Basidiospores 7.7-9.7 × 4.5-5.8 μm. The ITS sequences differ from the sequences of other species of section Colymbadini by at least fifteen substitutions and eight indel positions.

Holotype.

China. Jilin Province: Antu County, Liangjiang Town, Dongfanghong Village, broadleaf forest ( Quercus mongolica dominated forest with some Juglans and Acer ), 42°42'51"N, 128°01'10"E, alt. 640 m, 5 August 2017, M.L. Xie, HMJAU44449, GenBank No. (ITS) MK552380.

Etymology.

The name refers to the Laccaria -like lamellae when young.

Description.

Pileus 2.2-6.6 cm in diam., conical when young, then convex, strongly hygrophanous, reddish-brown (9E6-8), dark brown at the centre (8F6-8), margin to half-way translucently striate, rarely fibrillose, margin thin and wavy. Lamellae subadnate to emarginated, distant, Laccaria -like ( Laccaria laccata (Scop.) Cooke) when young, reddish-brown (9E6-8) to rusty brown (6E8), edge slightly serrate. Stipe 4.2-6.6 cm long, 0.4-0.8 cm thick at apex, 0.2-0.5 cm thick at base, cylindrical to tapering towards base, dark brown (7F6) to black brown (7F3), surface with white fibrillose when young, these disappearing with age (excluding the base of stipe). Universal veil white, extremely sparse, soon disappearing. Context dark brown (7F6-8), strongly hygrophanous (pileus and stipe). Odour indistinct. Exsiccata brown (5F8) to black brown (7F5). UV fluorescence yellow on stipe, pileus and lamellar edge, strong at stipe base.

Basidiospores 7.7-9.7 × 4.5-5.8 μm, Q = 1.43-1.84, 'X = 8.0-8.5 × 4.8-5.2 μm, 'Q = 1.66-1.70 (60 spores, 2 specimens), ellipsoid to amygdaloid, moderately and sharply verrucose, moderately dextrinoid. Basidia 4-spored, clavate, 23-39 × 7-9 μm, thin-walled, hyaline to olivaceous brown in 5% KOH. Lamellar edge fertile, with cylindrical-clavate sterile cells, 14-41 × 7-17 μm, thin-walled, hyaline in 5% KOH. Lamellar trama hyphae regular, pale olivaceous to olivaceous brown in 5% KOH, finely and densely encrusted. Pileipellis: epicutis hyphae cylindrical, 4-9.5 μm wide, dark olivaceous brown in 5% KOH, encrusted; hypocutis well developed, hyphae 11.5-53 μm wide, sub-cellular to cylindrical, slightly olivaceous in 5% KOH, finely encrusted. Pileus trama hyphae thin-walled, hyaline to slightly olivaceous in 5% KOH, smooth to finely encrusted. Clamp connections present.

ITS sequence.

The ITS sequences of two specimens are 534 bp long and 100% identical. They differ from the sequences of other species of section Colymbadini ( Niskanen et al. 2013a; Dima et al. 2014; Ammirati et al. 2017) by at least fifteen substitutions and eight indel positions.

Ecology and distribution.

In broadleaf forest ( Quercus mongolica dominated forest). Gregarious. Known from Jilin Province, China.

Additional specimens examined.

China. Jilin Province: Antu County, Liangjiang Town, Dongfanghong Village, broadleaf forest ( Quercus mongolica dominated forest with some Juglans and Acer ), 42°42'51"N, 128°01'10"E, alt. 640 m, 5 August 2017, M.L. Xie, HMJAU44450, GenBank No. (ITS) MK552381.

Comments.

Cortinarius laccariphyllus has strongly hygrophanous basidiomata, Laccaria -like (when young), with distantly-spaced lamellae and an extremely sparse, white veil. Morphologically, C. nolaneiformis (Velen.) Dima, Niskanen & Liimat. is similar to C. laccariphyllus due to the strongly hygrophanous pileus, similar colouration and similar size of spores. Cortinarius uraceomajalis Dima, Liimat., Niskanen & Bojantchev is also similar to C. laccariphyllus because of the black brown stipe and the striate pileus. However, both C. nolaneiformis and C. uraceomajalis have a yellowish veil and medium-spaced lamellae and lamellae not Laccaria -like. Furthermore, C. nolaneiformis is associated with broadleaf trees and also occurs in coniferous forest; C. uraceomajalis has a somewhat lighter brown pileus as well as generally smaller (av. 7.8-8.1 × 4.6-4.7 μm) and narrower (Qav.> 1.7) spores ( Dima et al. 2014). In the phylogenetic analyses, C. laccariphyllus was well separated from other species in section Colymbadini .

Figures

Figure 2. Basidiocarps of three newly-described species. a, b Cortinarius laccariphyllus (a, b HMJAU 44449, holotype); c, d Cortinarius neotorvus (c HMJAU 44441, holotype; d HMJAU 44439); e, f Cortinarius subfuscoperonatus (e HMJAU 44444, holotype; f HMJAU 44445). Scale bars: 2 cm (a, b, d-f). Photographs by Meng-Le Xie.

Figure 3. Basidiospores of three newly-described species. a Cortinarius laccariphyllus (HMJAU 44449, holotype); b Cortinarius neotorvus (HMJAU 44441, holotype); c Cortinarius subfuscoperonatus (HMJAU 44444, holotype). Photographs by Meng-Le Xie.

Figure 4. Margin cells of three newly-described species. a Cortinarius laccariphyllus (HMJAU 44449, holotype); b Cortinarius neotorvus (HMJAU 44441, holotype); c Cortinarius subfuscoperonatus (HMJAU 44444, holotype). Photographs by Meng-Le Xie.