Nothophoma garlbiwalawarda E.C. Keirnan, M.H. Laurence, R.G. Shivas & Y.P. Tan, 2021

Keirnan, Elizabeth C., Tan, Yu Pei, Laurence, Matthew H., Mertin, Allison A., Liew, Edward C. Y., Summerell, Brett A. & Shivas, Roger G., 2021, Cryptic diversity found in Didymellaceae from Australian native legumes, MycoKeys 78, pp. 1-20 : 1

Nothophoma garlbiwalawarda E.C. Keirnan, M.H. Laurence, R.G. Shivas & Y.P. Tan sp. nov. Fig. 4

Type.

Australia, South Australia, Wudinna, Senna artemisioides , 19 Aug. 2017, E.C. Keirnan (holotype BRIP 69595, includes culture ex-type).

Description.

Colonies on OA, 27-30 mm diam. after 7 d, flat with scant aerial mycelia with a few zonate rings, vinaceous to dark vinaceous; vinaceous to dark vinaceous; on MEA, 23-25 mm after 7 d, margin entire, flat, scant aerial mycelium towards centre, amber with abundant pycnidia; reverse amber darker towards centre; on PDA, 28-30 mm after 7 d, margin irregular, flat with aerial mycelia tufted in centre, dark with abundant pycnidia in concentric rings, buff at margin; reverse dark becoming buff at margin. NaOH spot test: reddish. Conidiomata pycnidial, globose to subglobose, 130-320 μm diam., pale brown, scattered, abundant, zonate, glabrous, non-papillate; ostiole c. 25 μm diam.; pycnidial wall composed of textura angularus, pale to medium brown, cells 5-12 μm diam. Conidiogenous cells phialidic, cylindrical, thin-walled, hyaline 5-12 × 2-4 μm long, narrower at the apex. Conidia aseptate, 5-7.0 × 2.0-3.0 μm, parallel to narrowly ellipsoidal, hyaline, wall c. 0.5 μm.

Etymology.

From the native language of the Indigenous Australian Barngarla people, meaning leaf-fun-guy. The Barngarla people are from the Eyre Peninsula region, which includes Wudinna, the locality where the holotype was collected.

Additional material examined.

Australia, South Australia, Adelaide, Senna artemisioides , 26 Oct. 2016, E.C. Keirnan (BRIP 69580); Berri, Senna artemisioides , 01 Jul. 2017, E.C. Keirnan (BRIP 69586); ibid, 01 Jul. 2017, E.C. Keirnan (BRIP 69587); Kimba, Senna artemisioides , 17 Sep. 2017, E.C. Keirnan (BRIP 69594).

Notes.

Nothophoma garlbiwalawarda is phylogenetically closest to No. anigozanthi and two novel species (see below for notes) (Fig. 2 ). Nothophoma garlbiwalawarda is distinguished from No. anigozanthi by its larger conidia (cf. 3.5-5 × 1.5-2.5 μm), rpb2 sequence (93% identity), and its reaction to NaOH spot test on MEA (dull green then black).

Figures

Figure 2. Epicoccum djirangnandiri: a leaf lesions on Swainsona galegifolia b 14 - d old colonies on PDA, MEA, OA (left, top to bottom) and lower surface (right) c upper surface d pycnidia on CLA e conidia. Scale bars: 200 µm (d); 7 µm (e).

Figure 4. Nothophoma garlbiwalawarda: a pin-prick leaf spots on Senna artemisioides from Wudinna SA b 12 - d old colonies top to bottom on PDA, MEA, OA (left, top to bottom) and lower surface (right) c upper surface d pycnidia on CLA e pycnidia and pycnidial ooze on OA f pycnidia on PDA g conidia. Scale bars: 300 µm (d, e, f); 7 µm (g).