Lactuca sativa

Berek-Nagy, Peter Janos, Gergo Toth, Szilvia Bosze, Lilla Borbala Horvath, Andras Darcsi, Sandor Csikos, Daniel G. Knapp, Gabor M. Kovacs & Imre Boldizsar, 2021, The grass root endophytic fungus Flavomyces fulophazii: An abundant source of tetramic acid and chlorinated azaphilone derivatives, Phytochemistry (112851) 190, pp. 1-11 : 9

4.5.1. Lactuca sativa seed bioassay

Lactuca sativa L. var. capitata ‘Attrakcio´’ (R´edei Kertimag, Hungary) seeds were washed twice with DW and were placed into Petri dishes (90 × 15 mm) containing filter paper (ø 75 mm, VWR, Hungary) (5 seeds in all Petri dishes). Dilution series of the isolated compounds were made in the concentration range of 1.0–100 μM by DW. The seeds were treated with 1.9 mL amounts of these solutions for 5 days at room temperature on natural light (the control was treated with DW). The length of the root and the hypocotyl was then measured by the software ImageJ (NIH, USA) using the screened pictures of the seedlings.