Neotorula nujiangensis S.C. He, D.P. Wei & Jayaward., 2024

He, Shu-Cheng, Wei, De-Ping, Bhunjun, Chitrabhanu S., Apurillo, Carlo Chris S., Thiyagaraja, Vinodhini, Jayawardena, Ruvishika S., Zhao, Yun-Wei & Zhao, Qi, 2024, A new species of Neotorula (Pleosporales) from the Southwest, China, Phytotaxa 662 (3), pp. 251-261 : 255-256

publication ID	https://doi.org/10.11646/phytotaxa.662.3.4
persistent identifier	https://treatment.plazi.org/id/160D766E-FFC1-FFCE-FF17-CD6BFD9EA2D3
treatment provided by	Felipe (2024-12-18 15:54:58, last updated 2024-12-18 16:03:06)
scientific name	Neotorula nujiangensis S.C. He, D.P. Wei & Jayaward.
status	sp. nov.

Treatment

Neotorula nujiangensis S.C. He, D.P. Wei & Jayaward. , sp. nov. Fig. 3 View FIGURE 3

Index Fungorum number: IF901395, Facesoffungi number: FoF 15220.

Etymology: Referring to the collecting site of the type specimen, Nujiang City, Yunnan, China.

Holotype: HKAS 131234 View Materials

Saprobic on dead wood. Asexual morph: Hyphomycetous. Colonies effuse on natural substrate, scattered, hairy, brown to black. Mycelium mostly immersed, branched, septate, smooth, brown to black. Conidiophores macronematous, erect, complex, straight, branched, guttulate, thick-walled, smooth-walled, multi-septate, frequently with irregular branches, pale brown to brown, lighten color towards the apex, 100–140 × 14–18 μm (x̄ = 120 × 16 μm, n = 10). Conidiogenous cells polyblastic, terminal, subglobose to globose, verruculose, pale brown to brown, thick-walled. Conidia solitary, pleuroacrogenous, simple, dry, monilioid, guttulate, thick-walled, 0–5-septate, pale brown to dark brown, verruculose, 17.2–32.6 × 13.0–18.7 μm (x̄ = 25.0 × 15.8 μm, n = 30). Sexual morph: Not observed.

Culture characteristics: Conidia germinating within 24 h on PDA at 25 °C, reaching 3.5–3.7 cm after 45 days incubation, above colony brown; reverse black with white edge, entire edge, flat, surface rough, mycelia dense, no pigment.

Distribution: Nujiang City, Yunnan Province, China.

Material examined: China, Yunnan Province, Nujiang City , (26°32′N, 98°43′E, 2450 m), on dead wood, 7 July 2022, Shu-Cheng He, HSC613 About HSC ( HKAS131234 View Materials , holotype) GoogleMaps ; ex-type, KUNCC 23–12855 . ibid. HSC941-1 About HSC , ( HKAS131235 View Materials , paratype) GoogleMaps ; ex-paratype, KUNCC23-16668 .

Notes: Our isolates ( HKAS 131234 and HKAS 131235) formed a monophyletic clade with Neotorula aquatica and N. submersa with 100 ML /1.00 PP statistical support ( Figure 2 View FIGURE 2 ). Neotorula nujiangensis resembles N. aquatica and N. submersa in having pleuroacrogenous conidia ( Hyde et al. 2016; Su et al. 2016). However, Neotorula nujiangensis compared with N. aquatica has longer and wider conidiophores (120 × 16 μm vs 41 × 6.5 μm and 120 × 16 μm vs 33 × 4.5 μm) and conidia (25 × 15.8 μm vs 13.5 × 5.5 μm and 25 × 15.8 μm vs 19 × 5.5 μm). Neotorula nujiangensis and N. submersa were from decaying wood in a terrestrial habitat while N. aquatica reported from an aquatic environment. Neotorula nujiangensis differs from N. aquatica by 0.49 % ITS, 0.28 % 28S and 0.62 % 18S differences in the nucleotides. Neotorula nujiangensis differs from N. submersa by 6.56 % ITS and 0.24 % 28S differences in the nucleotides. Thus, based on phylogenetic, morphological and ecological approaches, we propose our specimen as a new species, Neotorula nujiangensis .

References

Hyde, K. D., Hongsanan, S., Jeewon, R., Bhat, D. J., McKenzie, E. H. C., Jones, E. B. G., Phookamsak, R., Ariyawansa, H. A., Boonmee, S., Zhao, Q., Abdel-Aziz, F. A., Abdel-Wahab, M. A., Banmai, S., Chomnunti, P., Cui, B. K., Daranagama, D. A., Das, K., Dayarathne, M. C., de Silva, N. I., Dissanayake, A. J., Doilom, M., Ekanayaka, A. H., Gibertoni, T. B., Goes-Neto, A., Huang, S. K., Jayasiri, S. C., Jayawardena, R. S., Konta, S., Lee, H. B., Li, W. J., Lin, C. G., Liu, J. K., Lu, Y. Z., Luo, Z. L., Manawasinghe, I. S., Manimohan, P., Mapook, A., Niskanen, T., Norphanphoun, C., Papizadeh, M., Perera, R. H., Phukhamsakda, C., Richter, C., Andre, A. L. C. M., Drechsler-Santos, E. R., Senanayake, I. C., Tanaka, K., Tennakoon, T. M. D. S., Thambugala, K. M., Tian, Q., Tibpromma, S., Thongbai, B., Vizzini, A., Wanasinghe, D. N., Wijayawardene, N. N., Wu, H. X., Yang, J., Zeng, X. Y., Zhang, H., Zhang, J. F., Bulgakov, T. S., Camporesi, E., Bahkali, A. H., Amoozegar, M. A., Araujo-Neta, L. S., Ammirati, J. F., Baghela, A., Bhatt, R. P., Bojantchev, D., Buyck, B., da Silva, G. A., de Lima, C. L. F., de Oliveira, R. J. V., de Souza, C. A. F., Dai, Y. C., Dima, B., Duong, T. T., Ercole, E., Mafalda-Freire, F., Ghosh, A., Hashimoto, A., Kamolhan, S., Kang, J. C., Karunarathna, S. C., Kirk, P. M., Kytovuori, I., Lantieri, A., Liimatainen, K., Liu, Z. Y., Liu, X. Z., Lucking, R., Medardi, G., Mortimer, P. E., Nguyen, T. T. T., Promputtha, I., Raj, K. N. A., Reck, M. A., Lumyong, S., Shahzadeh-Fazeli, S. A., Stadler, M., Soudi, M. R., Su, H. Y., Takahashi, T., Tangthirasunun, N., Uniyal, P., Wang, Y., Wen, T. C., Xu, J. C., Zhang, Z. K., Zhao, Y. C., Zhou, J. L. & Zhu, L. (2016) Fungal diversity notes 367 - 490: taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity 80: 1 - 270. https: // doi. org / 10.1007 / s 13225 - 016 - 0373 - x

Su, H. Y., Hyde, K. D., Maharachchikumbura, S. S. N., Ariyawansa, H. A., Luo, Z. L., Promputtha, I., Tian, Q., Lin, C. G., Shang, Q. J., Zhao, Y. C., Chai, H. M., Liu, X. Y., Bahkali, A. H., Bhat, J. D., McKenzie, E. H. C. & Zhou, D. Q. (2016) The families Distoseptisporaceae fam. nov., Kirschsteiniotheliaceae, Sporormiaceae and Torulaceae, with new species from freshwater in Yunnan Province, China. Fungal Diversity 80: 375 - 409. https: // doi. org / 10.1007 / s 13225 - 016 - 0362 - 0

Figures

FIGURE 2. PHI analysis of combined 28S, 18S, tef1, ITS and rpb2 sequence data for N. nujiangensis (HKAS 131234) and related species of Neotorula. PHI results (Fw = 1.0) indicate no significant recombination with the dataset. New taxon is shown in red.

FIGURE 3 Neotorula nujiangensis (HKAS 131234, holotype) a Host substrate. b Colonies on natural substrate. c–f Conidiophores giving rise to conidiogenous cells. g-r Conidial chains. s, w Conidia. x, y Culture on PDA. Scale bars: d, e, s–w = 10 μm, c, f–i, p–r = 25 μm, j–o = 50 μm, b = 200 μm.