Pestalotiopsis multicolor Y. F. Wang & C. J. You, 2024

Wang, Yifeng, Tsui, Kin-Ming, Chen, Shimei & You, Chongjuan, 2024, Diversity, pathogenicity and two new species of pestalotioid fungi (Amphisphaeriales) associated with Chinese Yew in Guangxi, China, MycoKeys 102, pp. 201-224 : 201

Pestalotiopsis multicolor Y. F. Wang & C. J. You sp. nov.

Fig. 6

Etymology.

In reference to the multicoloured median cells.

Holotype.

BJFC-S1956.

Conidiogenesis.

Conidiophores reduced to conidiogenous cells, short, subcylindrical and hyaline. Conidiogenous cells were discrete, ampulliform, thin-walled, hyaline, smooth. Conidia fusiform to clavate, straight or slightly curved, olivaceous to brown, 4-septate, 14.5-20 × 3.5-5.5 μm, with apical and basal appendages. Basal cell obconic, hyaline, thin-walled, smooth, 2-4.5 µm; the three median cells dolioform, versicolor, pale brown to brown with septa darker than the rest of the cells, 9.5-13 µm, the second cell from base 2.5-4.5 µm; the third cell 3-4.5 µm; the fourth cell 3-4 µm; apical cell 2.5-3.5 µm, cylindrical, hyaline; 2-3 (mostly 3) tubular apical appendages, arising from the apex of the apical cell each at a different point, filiform, 8-16 µm; basal appendage present most of the time, single, tubular, unbranched, 2.5-4.5 µm (Fig. 6c-e ). Sexual morph not observed.

Culture characteristics.

Colonies on PDA reaching 90 mm diameter after seven days at 25 °C, with an undulate and radial edge, white aerial mycelium on surface flat or raised (Fig. 6a ). Conidiomata acervular in culture on PDA, globose, 200-800 μm in diameter, solitary or aggregated in clusters, exuding black conidial masses (Fig. 5b ).

Material examined.

China, Guangxi Province, from diseased needles of Chinese yew, May 2020, Y. F. Wang (BJFC-S1956, holotype); ex-type living culture CFCC59981, living cultures CFCC59982, CFCC59983, and CFCC59984 .

Notes.

The two isolates (CFCC59981 and CFCC59982) formed a distinct lineage with high support values (MP/ML = 100/100) in the phylogenetic tree. The morphology of the two isolates was distinctive within the Pestalotiopsis genus because the conidia were far smaller than those of any other species. In addition, the colour of median cells in our new collections changed from light concolorous to versicolorous. Therefore, four isolates in the present study were designated as a new species.

Figures

Figure 5. Pestalotiopsis taxicola (CFCC 59976) a culture on PDA b conidiomata formed on PDA c conidiogenous cells d-f conidia. Scale bars: 500 µm (b); 10 µm (c-f).

Figure 6. Pestalotiopsis multicolor (CFCC 59981) a culture on PDA b conidiomata form on PDA c conidiogenous cells d-f conidia. Scale bars: 500 µm (b); 10 µm (c-f).