Neoascochyta pseudofusiformis S. M. Fu & Yong Wang bis, 2024

Fu, Shamin, Sun, Jing-E, Tarafder, Entaj, Wijayawardene, Nalin N., Hu, Yan, Wang, Yong & Li, Yan, 2024, Pezizomycotina species associated with rotten plant materials in Guizhou Province, China, MycoKeys 106, pp. 265-285 : 265-285

Neoascochyta pseudofusiformis S. M. Fu & Yong Wang bis sp. nov.

Fig. 5

Etymology.

In reference to the fungus, which produced fusiform conidia morphologically similar to Neoascochyta fusiformis .

Diagnosis.

Neoascochyta pseudofusiformis is characterised by oval to fusiform conidia (3.6 × 1.9; L / W = 1.896) with moderate growth rate.

Type.

China, Guizhou Province, Guiyang City , 26 ° 57 ' N, 106 ° 72 ' E, from rotten dead branch, 19 July 2023, S. M. Fu, HGUP 23-0014 (holotype), ex-type culture GUCC 23-0045 .

Culture characteristics.

Colonies on PDA, 70–75 mm diam. after 7 d, margin regular, covered by floccose aerial mycelium, greyish-olivaceous, with flat and greenish-black flat mycelium near the margin; reverse black olivaceous. Mycelium is light to dark grey, separated, smooth, thin to thick wall. Acicular conidium, grey to dark grey, solitary or conjunctival, immersed in culture (WA), glabrous, subglobular, 100–250 × 90–130 μm, with a single pore neck; The angular textured cylindrical wall consists of 2 to 4 layers of flat polygonal cells 10–50 μm thick. The meristem cells are biparental, transparent, smooth-walled, pot or spherical, 3 × 5 μm wide. The conidia are 0–1 septum, transparent, smooth, thick-walled, mostly fusiform or slightly allantoic, 3.0–4.5 × 1.5–2.5 μm (x ̄ = 3.6 × 1.9 μm, n = 30).

Habit.

On rotten dead branches.

Distribution.

China, Guizhou Province, Guiyang City

Other materials examined.

China, Guizhou Province, Guiyang City , 26 ° 57 ' N, 106 ° 72 ' E, from rotten dead branches, 19 July 2023, S. M. Fu, HGUP 23-0014 , living culture GUCC 23-0045 , GUCC 23-0046 and GUCC 23-0047 .

Notes.

The present taxon differs morphologically to related species by conidial size range (3.0–4.5 × 1.5–2.5 μm vs. 16.5–27 × 5–8.5 μm in N. argentina and 2.5–3.5 × 1.0–1.5 μm in N. tardicrescens ) ( Chen et al. 2017; Valenzuela-Lopez et al. 2018). In phylogeny, our novel taxon maintained a close relationship to N. argentina CBS 112524 and N. tardicrescens CBS 689.97; however, DNA base differences (Table 2 ) supported that they belonged to different taxa.

Figures

Figure 5. Neoascochyta pseudofusiformis (GUCC 23-0045) a, b appearance on host surface c colonies on host surface d – g culture characteristics on PDA, WA (d, f above view e, g reverse view) h colonies on WA i – k conidiophores, conidiogenous cells and conidia l conidia. Scale bars: 20 µm (i – l).