Leptographium breviuscapum M.L. Yin, Z.W. de Beer & M.J. Wingf.

Yin, Mingliang, Wingfield, Michael J., Zhou, Xudong, Linnakoski, Riikka & Beer, Z. Wilhelm de, 2019, Taxonomy and phylogeny of the Leptographium olivaceum complex (Ophiostomatales, Ascomycota), including descriptions of six new species from China and Europe, MycoKeys 60, pp. 93-123 : 93

Leptographium breviuscapum M.L. Yin, Z.W. de Beer & M.J. Wingf. sp. nov. Fig. 3

Etymology.

The epithet (brevius-, short, and -scapum, branch) refers to very short conidiophores.

Type.

CHINA, Qinghai province, from Picea crassifolia infested with Polygraphus poligraphus , Aug. 2010, M.L. Yin & X.D. Zhou, (PREM 60914 holotype, ex-holotype cultures CBS 136507 = CMW 38888); Qinghai province, from Picea crassifolia infested with P. poligraphus , Aug. 2010, M.L. Yin & X.D. Zhou, (PREM 60915 paratype, ex-paratype cultures: CBS 136508 = CMW 38889).

Description.

Sexual state not observed. Conidiophores occasionally observed on wood of WA, macronematous, synnematous, short, wide at the stipe, light brown to yellowish, expanding branches at the apex, 150-230 μm in length including conidiogenous apparatus, 20-25 μm wide at base, 40-45 μm wide at apex, 100-150 μm wide at conidiogenous apparatus. Conidiogenous cells discrete, hyaline, cylindrical, percurrent proliferation, (8 –)9–13(– 15) × 1.8-2.5 μm. Conidia hyaline, one-celled, smooth, ellipsoidal, (3.7 –)4–4.5(– 5) × 2.5-3 μm. Culture characteristics: Colonies on OA, hyaline at first, later becoming light yellowish in the center, mycelium superficial on agar. Mostly mycelium observed in culture, synnemata sparse. Optimal temperature for growth 25 °C, growth reduced at 10 °C and 30 °C, no growth at 35 °C.

Host tree.

Picea crassifolia .

Insect vector.

Polygraphus poligraphus .

Distribution.

Qinghai, China.

Note: The asexual state of L. breviuscapum has very short conidiophores making it very easy to distinguish from that of other species in the complex.

Additional material examined.

Qinghai province, from Picea crassifolia infested with Polygraphus poligraphus , Aug. 2010, M.L. Yin & X.D. Zhou, (culture: CMW 38890). Yunnan province, from Pinus yunnanensis infested with Tomicus yunnanense , Sep. 2017, M.L. Yin, (culture: SCAU-475). Yunnan province, from Pinus yunnanensis infested with Tomicus yunnanense , Sep. 2017, M.L. Yin, (culture: SCAU-478).

Figures

Figure 3. Leptographium breviuscapum sp. nov. (CMW 38888) a fourteen-days old culture on OA with black background b synnematous asexual state on wood tissue on WA c-d conidiophore e conidiogenous cells f conidia. Scale bars: 100 μm (b), 25 μm (c), 25 μm (d), 10 μm (e), 5 μm (f).