Wyeomyia (Hystatomyia) chocoensis Porter & Wolff

Porter, Charles H., Wolff, Marta I. & E, 2004, A new species of Wyeomyia (Hystatomyia) (Diptera: Culicidae) from Colombia and a redescription of Wy. (Hystatomyia) intonca Dyar & Knab, Zootaxa 477, pp. 1-31 : 3-15

Wyeomyia (Hystatomyia) chocoensis Porter & Wolff , sp.n.

( Figs. 1 , 2 , 5 , 6 )

MALE. Head: Vertex and adjoining lateral surface of head with broad, dark decumbent scales with bronze and blue­green iridescence; no erect scales present; patch of white scales occurs basolaterally; scales along ocular line white or with at least their distal margin white resulting in distinct white line along edge of compound eye. Ocular setae dark brown, including 2 long approximated interocular setae. Interocular space without scales, narrows to width equal to or slightly less than that of single ommatidium. Clypeus and frons without setae or scales; frons with dense covering of fine aculeae (pubescence) between postfrontal suture and antennal socket; clypeus with less dense covering of minute aculeae. Palpus two­segmented (0.16–0.19 mm, mean 0.18, n 6); segment 1 short, rather quadrate; segment 2 elongate; both covered with dark scales. Proboscis slightly expanded in distal 0.3; dorsal surface with dark scales with blue­green iridescence; ventral surface primarily with pale and white scales extending from base to about 0.7 length where they merge with prominent preapical patch of bright white scales at beginning of apical expansion; white scales end at about 0.8, replaced by dark scales to distal end. Proboscis (P) (1.46–1.55 mm, n 3) longer than antennae (flagellum [F] 1.27–1.34 mm, mean 1.31, n 4), mean P:F 1.13 (n 3), but shorter than forefemur (Fe­I), mean P:Fe­I 0.74 (n 3). Pedicel with 6 small setae dorsomesad. Flagellum moderately verticillate, whorls with 8–11 setae, longest setae about 0.35 flagellum length; flagellomere 1 with dorsomesad cluster of 7–12 scales; length of selected flagellomeres (Flm) derived from 3 specimens (6 antennae) as follows: Flm5 0.08–0.09 mm, Flm11 0.10–0.12 mm, Flm12 0.12–0.15 mm, Flm13 all 0.19 mm; mean Flm13:Flm5 2.31. Distal 2 flagellomeres not disproportionately longer than preceding flagellomeres. Thorax: Integument primarily light brown. Scutum with broad dark scales with bronze and blue­green iridescence, anterior promontary region with a few white scales and about 9 dark brown setae, no acrostichal or dorsocentral setae present. Supraalar and antealar areas have combined sum of 25–31 (mean 28, n 3) dark brown setae. Scutellum covered with broad scales concolorous with those of scutum, margin of median lobe with 4 large and about 3 small dark brown setae, lateral lobes with 4 large and 4 or 5 small dark brown setae. Mesopostnotum brown with medial cluster of 8–11 brown setae. Antepronotal lobes widely separated dorsally, with dark scales dorsally and silvery white scales on portion adjoining pleura; each lobe with 7–12 dark brown setae. Postpronotum without setae; covered primarily with silvery white scales, those along dorsal margin concolorous with scales of scutum. Pleuron with silvery white scales although anterior half of mesokatepisternum and posterior one­third of mesanepimeron bare. Paratergite, meron, and metapleuron entirely bare. Pleural chaetotaxy as follows: prespiracular, 1 dark seta; postspiracular setae absent; upper proepisternal, 2–4 yellow setae; lower mesokatepisternal, 4–7 yellow setae; upper mesokatepisternal setae absent; prealar, 3–4 dark, occasionally pale, setae; lower mesepimeral setae absent; upper mesepimeral, 6– 9 yellow setae. Legs: forecoxa with silvery white scales on anterior margin, otherwise bare; midcoxa and hindcoxa with silvery white scales. Trochanters primarily with silvery white scales although few on dorsal surface, small dark scales on upper distal margin of midtrochanter and hindtrochanter, scattered dark scales on upper surface of foretrochanter. Dorsal surface of femora with dark scales with bronze reflection and dark blue and greenish­blue iridescence depending upon angle of incident light. Posteroventral surface of forefemur covered with white scales over basal 0.3; white scaling gradually decreases to narrow posteroventral line at about 0.5, which extends distally and becomes slightly broader near distal end. Ventral surface of midfemur and hindfemur with white scaling over entire length. Forefemur slightly longer than foretibia (Ti­I) (mean Fe­I:Ti­I 1.08, n 3); forefemur somewhat shorter than midfemur (Fe­II) (mean Fe­I:Fe­II 0.87, n 3) but longer than hindfemur (Fe­III) (mean Fe­I:Fe­III 1.27, n 3); forefemur longer than proboscis (mean Fe­I:P 1.36, n 3). Dorsal surface of foretibia and foretarsomere 1 (Ta­I1) concolorous with forefemur; scales on dorsal surface of Ta­I2–5 dark, appearing dark blue at certain angles of incident light; foretibia with white scaling on posteroventral surface, diminished distally, seldom reaching distal end; ventral surface of Ta­I1­5 primarily with dark scales with metallic reflection; ungues equal, simple, dark but pale basally. Midfemur distinctly longer than midtibia (Ti­II) (mean Fe­II:Ti­II 1.56, n 3), dorsal surface of midtibia, and midtarsomere 1 (Ta­II1) concolorous with midfemur, posteroventral surface of midtibia and Ta­II1 with broad white scaling over entire length. Midtarsomere 2 (Ta­II2) entirely white scaled with exception of some grey to dark scales basally on anteroventral surface, seldom extending beyond 0.3 length of tarsomere; Ta­II3–5 completely white scaled except for a few grey to dark scales at distal end of Ta­II5. Ungues dissimilar; larger unguis stout, dark, curved to almost 90° angle, tip blunt, rounded; smaller unguis simple, dark. Hindfemur slightly longer than hindtibia (Ti­III) (mean Fe­III:Ti­III 1.05, n 3), hindtarsomere 1 (Ta­III1) somewhat longer than hindfemur (mean Ta­III1:Fe­III 1.13, n 3). Dorsal surface of hindtibia and Ta­III1&2 concolorous with hindfemur, dorsal surface of Ta­ III3–5 with dark scales with metallic reflection. Ventral surface of hindtibia with white scales limited to about basal 0.1, remainder primarily with pale scales with metallic reflection; ventral surface of Ta­III1 with a few pale scales basally but predominately with dark scales having metallic reflection; ventral surface of Ta­III2 with predominately pale scaling; ventral surface of Ta­III3–5 with dark scales having metallic reflection. Ungues unequal; longer unguis very slender (0.07–0.08 mm) with fine tip, about 2x length of shorter; both curved distally. Halteres with scabellum and pedicel pale (cream­colored), distal portion of pedicel and capitellum covered with dark scales with bronze and bluegreen iridescence. Wing: 2.10–2.40 mm, wing scales mostly decumbent and concolorous with scutum. Dorsal scales primarily spatulate, rather broad with rounded ends; R2, R3, R4+5, M1, and M2 with typical broad spatulate scales over entire length. In addition to broad spatulate scales, R1 has a row of smaller spatulate scales closely appressed to vein; 1A with somewhat narrower spatulate scales, which are closely appressed to vein and extend over almost entire length of vein before ending near wing margin. Sparse row of relatively narrow scales (plume scales) occurs on dorsal surface of M and radial sector (Rs). Ventral wing scales similar to dorsal scales but with plume scales on following veins: M3+4 except near distal end, distal 0.5–0.6 of CuA, and a few on basal 0.5 of R4+5 and basal ~0.5 of R1. Also, plume scales may be present on distal third of 1A. CuA and 1A without spatulate scales on ventral surface, thus only with distal plume scales. Ventral scales of M all spatulate. Vein R2 about 4–5 times longer than vein R2+3; vein 1A ends somewhat before junction of mcu and CuA. Alula with 9 or 10 piliform setae. Abdomen: Abdominal terga primarily with dark scales similar in color to those of scutum; lateral margins with silvery white scales, which form an essentially straight line along abdomen; pale scales expand dorsally somewhat on tergum VII; sterna covered with silvery white scales. Tergum I covered with dark scales dorsally, lateral margins bare; diffuse group of about 20–25 prominent, amber­colored setae occur laterally on either side of meson. Laterotergite without scales. Distal margin of terga II–V with about 7–14 small pale setae; these setae more numerous along distal margin of terga VI and VII (~ 22); lateral margins of terga II–VI usually with 1–4 small pale setae. Distal margin of sterna II–V with about 10–20 small pale setae; distal margin of sterna VI and VII (~23) with more numerous and somewhat longer setae; sternum VII also with 2 or 3 setae medially and slightly basal to distal margin; lateral margins of sterna III–VII usually with 3–6 small pale setae. Tergum VIII and sternum VIII are included in description of genitalia. Genitalia ( Fig. 1 C–G): Tergum VIII (ventral in position) narrow, 2.8–3.3x as wide as long; covered with small spicules, which become minute and very numerous basally although basal 0.2–0.4 glabrous; distal margin somewhat concave; spatulate scales primarily along lateral margins. Tergum VIII with 72–90 (mean 78, n 6) relatively long setae with curved tips, longest about 1.4x length of tergum VIII along median plane; setae located along and near distal margin but most numerous in mesal region where their presence is somewhat expanded basally to about 0.5 length of tergum VIII; a pair of tiny setae located about 0.33 from basal margin and laterad of median plane. Sternum VIII (dorsal in position) 2.0–2.5x as wide as long; distal margin somewhat convex; covered with small spicules, which become minute and very numerous basally although about basal 0.3 glabrous. Sternum VIII also covered with dark, decumbent spatulate scales and with 17–24 (mean 21, n 6) setae arranged primarily as single row along distal margin, longest setae similar in length to those on tergum VIII; pair of minute setae or punctures located sublaterad and about 0.4 from basal margin. Tergum and sternum IX fused laterally forming complete ring. Tergum IX bearing 3 or 4 stout but relatively short setae on either side of narrow median bridge, apices of setae bent slightly laterad. Sternum IX narrow but with medial triangular­shaped expansion between base of gonocoxites; outer surface densely spiculate. Gonocoxite spiculate; elongate, expanded basally, distal 0.5 slender and slightly bowed; sternal surface with scattered setae; lateral margin from near base to apex with scales and scattered setae; long, recurved, yellowish setae along mesal margin of slender distal portion of gonocoxite from slightly above base of gonostylus to near apex; apex with dense cluster of long, slightly lanceolate, dark setae (about 0.4 length of gonocoxite). Three tergal setal groups: (1) proximal mesal group comprised of a dense cluster (golden in color) of 25–30 (mean 28, n 8) lanceolate setae (about 0.4 length of gonocoxite), most curved beyond mid­length; (2) a tight group of 9–15 (mean 12, n 8) slender, pale setae (similar in length to lanceolate setae) at distal edge of lanceolate setal cluster; (3) slightly laterad of lanceolate setal cluster is a diffuse group of 13–17 (mean 16, n 9) very slender dark setae, curved near tip. Gonostylus ( Fig. 1 D) about 0.4 length of gonocoxite, arises on mesal surface of gonocoxite near mid­length, curved toward distal direction at about 0.3 length; glabrous but rugose near apex; broad over entire length but with short, acute tip. Aedeagus longer than wide; submedian tergal arms bend toward each other resembling an upside­down V, narrowly joined at midline to form a tergal bridge; apical tergal arms flared laterally and joined apically to form narrow apical tergal bridge (ATB), posterior margin of ATB minutely crenulate; median sternal plate located within apical tergal arms, apical portion coarsely denticulate. Proctiger (in lateral view) with broad basal sclerotization of tergum X, paraproct rounded apically with adjacent subapical lobe and with 4 or 5 cercal setae.

FEMALE. Like male except for sexual characters as follows. Head: Proboscis slightly expanded in distal 0.3, ventral surface with white scales from base to slightly beyond 0.7 length of proboscis (ending near base of apical expansion), about distal 0.3 of proboscis with dark scales. Thorax: Integument tan to light brown. Legs: Posteroventral surface of forefemur with white scaling over basal ~0.3, rather narrow line of white scales continues to distal end. Posteroventral surface of foretibia with white scales over entire length or to 0.7 and with scattered white scales to distal end. Foretarsomeres 1–5 (Ta­I1–5) with dark scaling, scales on posteroventral surface with bright metallic reflection. Foretarsomere 1 (Ta­I1) often with a few white scales basally. Midfemur and midtibia with white scales over entire length on posteroventral surface, although white scales may become scattered or absent toward distal end of midtibia. Dorsal surface of midtarsomere 1 (Ta­II1) primarily dark scaled with a few white scales at distal end; in some specimens, narrow irregular row of white scales extends over entire anterodorsal surface. Posteroventral surface of Ta­II1 with metallic reflection; white scales limited primarily to basal 0.3 but scattered white and pale scales extend to distal end. Dorsal surface of Ta­II2 ( Fig. 6 B) with white scaling, which gradually expands distally to anterior and posterior surfaces; ventral surface with dark scales with metallic reflection. midtarsomeres 3–5 (Ta­II3–5) primarily with white scales, dark scales limited to ventral surface; Ta­II5 with a few dark scales dorsally at distal end. Ventral surface of hindfemur with white scales over entire length. Ventral surface of hindtibia primarily with dark scales, some specimens have white scales on basal 0.2. Dorsal surface of hindtarsomeres 1–5 (Ta­III1–5) with dark scaling and metallic or blue iridescence depending upon angle of light. Presence of white scales on ventral surface of Ta­III1 variable; a few scattered white scales may be present or white scales may extend as line to about 0.6. Scales on ventral surface of Ta­III2–5 pale to dark with bright metallic reflection. Genitalia ( Fig. 5 E­H): Tergum VIII wider than long (width 0.41 mm, length along median plane 0.17 mm), covered with minute spicules and spatulate scales, lateral margins rounded, distal margin somewhat convex. Setae primarily limited to distal 0.33 of tergum VIII, most numerous near distal margin, extending somewhat basally on median plane, in total about 38–47 setae; many setae near distal margin quite long, some slightly exceeding length of tergum VIII; interspersed among long setae are short setae about 0.20–0.25 length of long setae; pair of tiny setae situated sublaterally near basal margin. Sternum VIII wider than long (width 0.37–0.43 mm, length along median plane 0.11–0.12 mm), covered with minute spicules and spatulate scales; distal margin broadly concave with numerous strong setae, which expand posteriorly to form broad V­shaped cluster over median plane, in total about 52–53 setae; length of many setae similar to that of sternum VIII (0.12 mm) although longest nearly twice that length; lateral margins with very few scales or setae; pair of tiny setae located sublaterally near basal margin. Tergum IX narrow (width 0.21–0.22 mm, length 0.03 mm), covered with small spicules, distal margin slightly convex with slight emargination at center, 1 or 2 prominent setae on either side of midline. Insula wider than long, covered with moderately long spicules, apex rounded, about 12 small setae near distal margin, basomesal semicircular depression present with spicules along lateral edges. Postgenital lobe similar in length to cerci, covered with minute spicules, apex slightly emarginate at center, ventral surface with numerous short setae over distal 0.6, dorsal surface with about 7 longer setae on either side of midline; dorsal postgenital lobe length 0.09 mm, dorsal postgenital lobe index 1.85–2.00 (see Reinert, 1974). Cercus rather flat, covered with minute spicules, apex rounded to somewhat truncate; dorsal surface with about 10–13 setae, longest 0.7–0.8 length of dorsal postgenital lobe. Three spherical spermathecal capsules, all somewhat different in diameter.

PUPA ( Fig. 1 A,B). Position and character of setae as figured; numbers of branches presented in Table 1. Overall color pattern of pupal exuviae remains similar among different individuals but variation occurs in intensity of pigmentation. Cephalothorax (CT): Tan with very pale to clear patches. Most notable pale areas on scutum include small spot at base of trumpet, two pale areas adjacent to antenna, and pale areas along dorsal margin of scutal protrusion associated with base of trumpet. Seta 1­CT strongly developed, long, double, often slightly bent or curved submedially or medially; 4­CT usually with 2 or 3 branches; 5­CT strongly developed, long, single. Mesothoracic wing mottled basally with 2 distinct pale areas, very pale distally. Metathoracic wing tan but mottled with pale areas; distinct clear spot on each lateral margin; narrow portion of metathoracic wing beyond pale spot (adjacent to lateral margin of abdominal segment I) quite darkly pigmented. Trumpet: Tan, darker basally and near distal end, medial portion very pale (almost white), distal end slightly flared and paler at tip (length 0.80–1.06 mm, mean 0.93 mm, n 8). Abdomen: Coloration varies from very light to dark tan. Abdominal tergum I mottled with pale spots corresponding to positions of seta 3­I, 4­I, and 6,7­I. Pale spot associated with seta 6,7­I large, often very prominent. Abdominal sternum I frequently with submesal puncture. Abdominal tergum II dark tan with pale areas/spots laterally and distally; seta 2,3­II within distal submesal pale area and seta 1,5­II at distal edge of this pale area; seta 4­II within pale spot and seta 6­II within extensive lateral pale area. Abdominal tergum III similar in pigmentation to abdominal tergum II but with distinct submesal pale area, which extends to distal margin, seta 1– 3 ­III within this pale area; seta 4­III within pale spot; seta 6­III within extensive lateral pale area. Areas of darker coloration, most prominent as mesal and submesal longitudinal lines, are progressively diminished from abdominal terga IV to VII. Abdominal terga II–V with sublateral pale area separated by narrow dark line from relatively broad pale area along lateral margin. Abdominal tergum VIII tan but with basomesal region darker. Seta 1­I usually with 13–17 branches, 1­II–VI prominent, multibranched; seta 2­II very small, basal or basolateral to 1­II, 2­III–VII very small, basomesal or mesal to 1­III–VII; seta 3­I rather strong, usually single, 3­II,III single, long (approx. 0.8 mm), 3­IV–VI about 0.3 length of 3­III, 3­IV,VI usually 2­branched, 3­V usually with 3 or 4 branches; seta 5­I double, infrequently triple; seta 5­II,III relatively small (approx. 0.2 mm), 5­II 3­branched, less frequently 2­ or 4­branched, 5­III with 3 or 4 branches, 5­ IV–VI single, very long (approx. 1.0– 1.1 mm). A puncture, situated near seta 4 on abdominal segments III–V, is usually located distolateral to 4­III, distal to 4­IV, and basomesad to 4­V. Intersegmental sclerites associated with abdominal terga II­VI tan to dark tan. Paddle: Pale tan, midrib not perceptibly darker; lateral margin spiculate from near base to apex, mesal margin similar but with few spicules basally. Male genital lobe: Tan, large (length [l] 0.54–0.57 mm, mean 0.56 mm, n 8; width [w] 0.40–0.46 mm, mean 0.43, n 8; mean l:w 1.30, range 1.23–1.37) but rather narrow; its width being similar to combined width of paddles.

Cephalothorax Abdominal segments

Seta no. CT I II III IV V VI VII VIII

0 – – 1 1 1 1 1 1 1 1 Range followed in parenthesis by mode; based on 8 specimens (16 setae). 2 One exception from number in parenthesis.

3 Usually absent, 2­branched when present.

4 Often only puncture present.

Head Thorax Abdominal segments 1 Range followed in parenthesis by mode; based on 8 specimens (16 setae). 2 One exception from number in parenthesis.

FOURTH­INSTAR LARVA ( Fig. 2 ). Position and character of setae as figured; numbers of branches presented in Table 2 . Head: Slightly wider than long and light tan. Collar absent. Slits of occipital foramen extending laterally to near base of pigmented area representing compound eye but not reaching lateral margin of head capsule. Margins of occipital foramen surrounding each slit darkly tanned, especially at thickened lateral end. Hypostomal suture complete, slightly curved, ending caudally at posterior tentorial pits (PTP). Posterior tentorial pits located about midway between seta 14­C and caudal margin of head capsule. Dorsomentum with 1 large central tooth and usually 9 (8–10) pairs of smaller lateral teeth. Maxilla (Mx): Mesal margin produced and rounded; maxillary brush quite long, maxillary brush spicules similar in length to seta 4­Mx (0.19 mm); 4­Mx stout and forked or branched near tip. Seta 1­Mx very short, stout (~ 0.012 mm); seta 2­Mx about 0.035 mm in length, basal to 1­Mx; seta 3­Mx absent but tiny papilla present; seta 6­ Mx prominent, single, about 0.06 mm in length. Mandible: Short, dorsal mandibular tooth with tip somewhat blunt, about twice as long as 3 teeth below it. Mandibular rake blade prominent, arising at base of mandibular teeth on ventral surface. Mandibular rake comprised of 8 or 9 stout spicules, each about 0.08 mm in length. Mandibular lobe with distinct basal protrusion from which cluster of spicules arise (Mandibular lobe spicules 1 [MLS1]). Seta 2­Mn single, about 0.33 longer than adjacent spicules of mandibular brush (MnB). Mandibular sweeper 1 (MnS1) comprised of about 6 long, slender spicules (~ 0.12 mm in length); mandibular sweeper 2 (MnS2) with 6–8 spicules similar in length to those of MnS1. Antenna: Short (0.25–0.29 mm, mean 0.27 mm, n 10), slender; seta 1­A 2­ branched (occasionally 3­branched or single) borne dorsally about 0.69 (mean of 10) from base and extends to or slightly beyond tip of antenna. Cranium setae: 1­C strongly developed, stout; 4–7­C multi­branched, 7­C weakly and 4–6­C weakly to moderately aciculate, 4­C slightly shorter than 5–7­C; 8,9­C multi­branched, very slender, similar in length; 12,13­C multi­branched, 13­C shorter and more slender than 12­C. Thorax: Seta 0 of prothorax (0­P) stellate, mesad of 4­P; 1­P stellate, weakly aciculate; 2­P quite long, weakly aciculate near base, slightly dorsal to 3­P; 5–7­P clustered together, 7­P slightly ventral to 5,6­P and about 0.4 length of 5­P; 9–12­P clustered together, 9­P very long, slightly more than twice length of 10­P, 12­P slightly longer than 10­P; 14­P forked or 2­branched, occasionally single. Seta 1 of mesothorax (1­M) prominent, stellate, branches weakly to moderately aciculate; 2,3­M single, 2­M often more than 0.5 length of 3­M, base of 2­M as wide or wider than base of 3­M; 5­M dorsomesad to 6,7­M, 7­M about 0.33 length of 5­M, 6­M slightly longer than 5­M; 9–12­M clustered together, 9­M slightly longer than 10­M, 12­M less than 0.5 length of 10­M; 13­M prominent, stellate, located on ventrolateral margin anterior to 9–12­M setal cluster; 14­M prominent, stellate with longest branches about 0.25–0.29 mm in length. Seta 1 of metathorax (1­T) prominent, stellate, branches weakly aciculate; 4­T strong, distal to 2,3­T; 5­T prominent, stellate, located about halfway between 2­T and 6­T; 6­T single, relatively small, similar in length to 12­T; 7­T with aciculate branches; 13­T prominent, branches weakly aciculate, longest branches about 0.52– 0.55 mm in length. Abdomen: Seta 1­I–VII well developed, stellate, weakly to moderately aciculate. Seta 2­I laterad of 1­I, strong, often 3­branched, basal to 3,4­I; 2­II single or 2­ branched, basal to 1­II; 2­III small, basal and slightly mesad of 1­III; 2­IV–VII small, distinctly mesad of 1­IV–VII. Seta 3­I mesad of 4­I, slender, about twice the length of 4­I; 3­ II laterad of 4­II; 3­IV,V slightly dorsal to 6­IV,V; 3­V long, about 0.8–1.0 length of shorter branch of 6­V; 3­VI mesad of 4­VI, similar in length to 3­I–IV; 3­VII very long, aciculate. Seta 4­I laterad of 3­I, usually 2­branched; 4­II slightly mesad of 3­II; 4­III,IV well basolaterad of 1­III,IV, very small, less than 0.5 length of 9­III,IV; 4­V laterad of 1­V, longer than 9­V; 4­VI slightly dorsad to 6­VI. Seta 6­I with 5 or 6 branches; 6­II–VI strongly developed, long; 6­II 3­branched, longest branch about 1.5 times or more length of longest 6­I branch; 6­ III–VI 2 ­branched; 6­VII relatively short, usually 4­branched. Seta 7­I usually 4­branched, similar in length to 6­I; 7­II 3­branched, shorter than longest branches of 6­II; 7­III 3­branched, usually less than 0.5 length of 6­III; 7­IV–VI multibranched, weaker than 9­IV–VI, 7­IV distinctly shorter than 9­IV but 7­VI similar in length to 9­VI. Seta 10­I single, mesad of 11­I ventrally; 10­II 2 ­branched, slightly distolaterad of 12­II; 10­III usually 3­branched, located between 6­III and 12­III; 10­IV with 2 or 3 branches, 10­V 3–5 ­branched; 10­VI frequently 5­branched, well mesad of 13­VI on ventral surface. Seta 11­I prominent, stellate, often slightly smaller than 13­I; 11­II–VI tiny, often with 2 or 3 branches. Seta 13­I–VII well developed, mesad of ventral setae with the exception of 12­IV,V, 10­VI, and 11­VII. Segment VIII: Comb plate absent; comb scales in about 4 irregular rows, proximal to distal gradation in scale size with smallest scales in proximal row (mean number of scales 65, range 53–72, n 8); individual scales relatively narrow with apical end somewhat flared and fringed. Seta 1­VIII stellate, slightly dorsad of dorsal most comb scale; all setae of segment VIII dorsal to origin of segment X; seta 2­VIII single, rather long, about 0.6 length of siphon; 4­VIII slender, usually 3­branched. Siphon: Long, slender (mean length 0.95 mm, range 0.86–1.04 mm, n 12), straight or very slightly curved distally, pigmentation uniformly light except darker along basal edge, surface smooth, siphon index 6.6–8.3 (mean 7.3, n 12). Pecten comprised of 4– 8 (mode 7, n 12) slender spine­like spicules, somewhat fringed apically; basal spicule often slightly distal to seta 1­S. Seta 1­S with 3 or 4 branches, located basally about 0.14 (mean of 11) of siphon’s length. Ventral accessory setae (1a­S) usually unbranched although penultimate seta sometimes 2­branched and distal seta infrequently 2­branched or forked; setae arranged in 2 rows, one on either side of siphon along ventral margin, approximated distally, corresponding setae of each row often paired; number of setae in 2 rows combined varied from 12–17 (mean 14, n 13); distal­most seta variable in length but often extends to or slightly beyond tip of siphon. Majority of dorsal accessory setae (2a­S) unbranched, although 2 distal­most setae 2­branched (infrequently single or 3­branched); in addition, setae 3 and 4 from distal end usually 2­branched and fifth seta from distal end occasionally 2­branched; distal seta does not reach tip of siphon; basal­most 2a­S seta tends to be shorter than adjacent setae, e.g., about 0.85 (range 0.72–0.95, n 12) length of third and fourth setae from base; setae arranged in 2 rows, one on either side of siphon along dorsal margin, approximated distally, corresponding setae of each row often paired; number of setae in 2 rows combined varied from 17–26 (mean 21, n 12). Seta 2­S strong, laterally compressed, slightly sinuate with hook­like apex; seta 6­S, 8­S, and 9­S single. Segment X: Saddle tan, darker than siphon; extends near to ventral surface, mean length measured dorsally 0.20 mm (n 10); surface of saddle smooth but minutely spinulate, saddle marginal spicules absent. Seta 1– 3 ­X well developed; seta 1,3­ X 2 ­branched, long; seta 2­X 3­branched (rarely 2­branched), smallest (upper) branch about 0.67 length of middle branch and usually about 0.25–0.33 length of lower branch; seta 4­X 7–11­branched (mode 8, n 24), without basal support plate.

ETYMOLOGY. The name chocoensis is derived from Chocó, the name of an Indian tribe in northwestern Colombia and the political division, Department of Chocó. Chocó also has been used in a biogeographic sense to define this region of exceptional endemicity and species richness; e.g., the Chocó refuge of Haffer (1974) and the Chocó phytogeographic region of Gentry (1982).

BIONOMICS. Larvae of Wy. chocoensis were found in tank bromeliads growing in a variety of coastal habitats in the geographic area of Ensenada de Utria and southward to Jurubida. This region is located along the northern Pacific Coast of the Department of Chocó, Colombia. Larvae of Wy. chocoensis were rather ubiquitous, occurring in bromeliads within mangrove, at the edge of mangrove and in adjacent forest. In most instances, only a few larvae were found in a given bromeliad, and they tended to be associated with smaller bromeliad plants, i.e., those holding between 100–600 ml of water. Bromeliads containing larvae of Wy. chocoensis varied in their height above ground level from 0.6 m to 12.5 m, the latter representing the highest plants sampled. No preferences were apparent with regard to the species of tank bromeliad used as a larval development site. Thus, larvae of Wy. chocoensis were found in all of the common tank bromeliads indigenous to the region ( Guzmania scherzeriana Mez, G. m u s a i c a [Linden & André] Mez, G. glomerata Mez & Wercklé , G. lingulata [L.] Mez, Werauhia ringens [Griseb.] J.R. Grant, W. gladioliflora [H. Wendland] J.R. Grant, W. sanguinolenta [Linden ex Cogniaux & Marchal] J.R. Grant, Aechmea dactylina Baker , and A. pubescens Baker ).

DISTRIBUTION. Wyeomyia chocoensis is known only from the coastal localities of the Department of Chocó, Colombia (Ensenada de Utria and southward to Jurubida), where the type specimens were collected.

MATERIAL EXAMINED.

Sixty­nine specimens (31ɗ, 5Ψ, 6ɗG, 3 ΨG, 4Le, 10 Pe, 10L), including 4 complete and 6 partial individual rearings. Holotype, ɗ (CO111­103), with dissected genitalia on microscope slide, COLOMBIA: Chocó, Ensenada de Utria (6°01.3'N 77°21.0'W), 15­II­ 1999, 0–10 m, hbt: Guzmania scherzeriana Mez , (Wolff & Porter ) ( USNM). Paratypes, COLOMBIA: Chocó, Ensenada de Utria (6°01.3'N 77°21.0'W), 15­II­1999 (1ɗ 1Ψ 1LePeΨ 1L – CO111­3, ­15, ­20), 0–10 m, hbt: Guzmania scherzeriana Mez , (Wolff & Porter ); same locality, 15­II­1999 (2ɗ 1Peɗ 1L – CO116­17, ­40, ­42), 0–10 m, hbt: Guzmania scherzeriana Mez , (Wolff & Porter ); same locality, 20­II­1999 (2ɗ 1ɗG 1Peɗ 2L – CO1175­1, ­101 with dissected genitalia, ­112), 0–10 m, hbt: Werauhia gladioliflora (H. Wendland) J.R. Grant , (Wolff & Porter ); same locality, 20­II­1999 (1ɗ 1ɗG 1LePeɗ – CO1176­110 with dissected genitalia), 0–10 m, hbt: Aechmea dactylina Baker , (Wolff & Porter ); same locality, 25­VI­1999 (2ɗ 1ɗG – CO11136­100, ­ 101 adult on microscope slide with dissected genitalia), 0–10 m, hbt: Werauhia sanguinolenta (Linden ex Cogniaux & Marchal) J.R. Grant , (Wolff & Porter ); Chocó, Ensenada de Utria (6°03.1'N 77°21.5'W), 17­II­1999 (5ɗ 1ɗG 1Ψ 1ΨG 1LePeɗ 1L – CO1141­ 11 adult on microscope slide with dissected genitalia, ­12, ­ 101 adult on microscope slide with dissected genitalia, ­106, ­ 108, ­110, ­111), 0–10 m, hbt: Guzmania scherzeriana Mez , (Wolff & Porter ); same locality, 18­II­1999 (1ɗ 1L – CO1145­13, ­104), 0–10 m, hbt: Guzmania scherzeriana Mez , (Wolff & Porter ); same locality, 18­II­1999 (2ɗ 1Peɗ – CO1148­14, ­101), 0–10 m, hbt: Werauhia ringens (Griseb.) J.R. Grant , (Wolff & Porter ); same locality, 18­II­1999 (1ɗ – CO1149­5), 0–10 m, hbt: Werauhia ringens (Griseb.) J.R. Grant , (Wolff & Porter ); same locality, 27­VI­1999 (1ɗ 1ɗG – CO11172­ 29 adult on microscope slide with dissected genitalia), 0–10 m, hbt: Werauhia ringens (Griseb.) J.R. Grant , (Wolff & Porter ); Chocó, Ensenada de Utria (6°03.0'N 77°20.2'W), 24­VI­1999 (1ɗ 1ɗG – CO11101­103 with dissected genitalia), 50 m, hbt: Guzmania scherzeriana Mez , (Wolff & Porter ); Chocó, Ensenada de Utria (6°03.1'N 77°21.0'W), 24­VI­1999 (2ɗ 1LePeɗ – CO11107­5, ­13), 50 m, hbt: Guzmania musaica (Linden & André) Mez , (Wolff & Porter ); same locality, 26­ VI­1999 (2L – CO11142­11, ­12), 50 m, hbt: Guzmania musaica (Linden & André) Mez , (Wolff & Porter ); same locality, 26­VI­1999 (2ɗ 1Ψ – CO11148­9, ­100, ­102), 50 m, hbt: Guzmania glomerata Mez & Wercklé , (Wolff & Porter ); same locality, 26­VI­1999 (1L – CO11153­3), 50 m, hbt: Guzmania musaica (Linden & André) Mez , (Wolff & Porter ); same locality, 26­VI­1999 (1L – CO11154­3), 50 m, hbt: Guzmania musaica (Linden & André) Mez , (Wolff & Porter ); Chocó, Ensenada de Utria (6°03.1'N 77°22.0'W), 28­VI­ 1999 (1ɗ 1Ψ – CO11178­100, ­101), 10 m, hbt: Aechmea pubescens Baker , (Wolff & Porter ); Chocó, Ensenada de Utria (6°02.8'N 77°22.6'W) 28­VI­1999 (3ɗ 1Ψ 1ΨG – CO11181­12, ­101 with dissected genitalia, ­106, ­110), 20 m, hbt: Guzmania lingulata (L.) Mez, (Wolff & Porter ); Chocó, Nuquí, Río Chori (5°50'N 77°17'W), 19­II­1999 (1Ψ 1ΨG – CO1171­101 with dissected genitalia), 0–10 m, hbt: Werauhia sanguinolenta (Linden ex Cogniaux & Marchal) J.R. Grant , (Wolff & Porter ); same locality, 19­II­1999 (1ɗ 1Peɗ – CO1172­100), 0–10 m, hbt: Werauhia gladioliflora (H. Wendland) J.R. Grant , (Wolff & Porter ); Chocó, Nuqui, Morro Mico (5°52'N 77°18'W), 21­IX­1999 (1ɗ 1Peɗ – CO11189­100), 10 m, hbt: Guzmania scherzeriana Mez , (Wolff & Porter ); same locality 22­IX­1999 (1ɗ 1Peɗ – CO11239­101), 10 m, hbt: Guzmania scherzeriana Mez , (Wolff & Porter ).

Figures

FIGURE 1. Pupa and male genitalia of Wyeomyia chocoensis. A, B, Pupa: (A) left side of cephalothorax, dorsal to right; (B) dorsal (left) and ventral (right) aspects of metathorax and abdomen, dorsal color pattern not illustrated for abdominal segments II­VIII. C – G, Male genitalia, aspects as indicated: (C) gonocoxite; (D) gonostylus; (E) proctiger (left side); (F) tergum 1 X; (G) aedeagus, with parameres and basal pieces attached.

FIGURE 2. Fourth­instar larva of Wyeomyia chocoensis. A, Head, dorsal and ventral aspects of left side; B, thorax and abdominal segments I­VI, dorsal and ventral aspects of left side; C, abdominal segments VII­X, left side. Abbreviations: C, cranium; CS, comb scale; Dm, dorsomentum; M, mesothorax; Mx, maxilla (ventral aspect); P, prothorax; PS, pecten spine; S, siphon; T, metathorax; I – X, abdominal segments; 1 – 15, setal numbers for specified areas.

FIGURE 5. Female genitalia of Wy. intonca (A – D) and Wy. chocoensis (E – H), aspects as indicated: (A, E) postgenital lobe and cerci; (B, F) sternum VIII; (C, G) insula; (D, H) tergum IX.

FIGURE 6. Female midleg tarsomeres 1 – 5, anterior surface aspect: (A) Wyeomyia intonca; (B) Wyeomyia chocoensis.

Tables

TABLE 2. Number of branches for larval setae of Wyeomyia (Hystatomyia) chocoensis.

Seta no.	C	P	M	T	I	II	III	IV	V	VI	VII	VIII	X
0	1	10–17 (13)	–	–	–	1	1	1	1	1	1	1	–
1	1	14–27 (19)	18–31 (24)	14–23 (21)	12–21 (13)	10–21 (13)	10–19 (11)	9–18 (11)	9–17 (11)	9–17 (11)	10–18 (14)	16–25 (20)	2
2	–	1	1	1,2 (1)	2–5 (3)	1,2 (1)	1	1	1	1	1	1	2,3 (3)
3	1	4–7 (5)	1	3,4 (3)	1,2 (1)2	1	1	1	1	1	1	7–12 (8)	2,3 (2)
4	6–13 (9)1	2	2,3 (3)	3–6 (4)	1–3 (2)	2–5 (2)	1–3 (1)	1–3 (2)	2–4 (3)	2	1	2,3 (3)	7–11 (8)
5	5–9 (7)	1	1	17–28 (19)	4–7 (5)	5–9 (6)	6–10 (7)	6–12 (7)	7–10 (7)	6–10 (6,7)	6–8 (6)	5–7 (5,6)	–
6	5–8 (6)	2,3 (2)2	1	1	5,6 (5)	3,4 (3)2	2	2	2	2	3–6 (4)	–	–
7	7–11 (8)	1	1,2 (1)	9–12 (9)	3–5 (4)	3,4 (3)2	3,4 (3)2	6–10 (8)	6–11 (6,8)	4–8 (6,7)	1	–	–
8	4–6 (5)	12–20 (14)	6–11 (7)	7–12 (8)	–	2,3 (2)	3–5 (3)	1–3 (2)	2–4 (3)	5–9 (6)	8–15 (11)	–	–
9	7–12 (9)	1	1	5–7 (5)	4–8 (6)	6–16 (7)	6–16 (8)	8–19 (11)	8–16 (11)	9–18 (9,10)	9–14 (12)	–	–
10	2–5 (3)	1	1	1	1,2 (1)2	1,2 (2)2	2,3 (3)	2–4 (3)	3–5 (4)	4–7 (5)	2	–	–
11	3–5 (4)	7–11 (9)	6–12 (7,8)	4–8 (5,6)	8–13 (10)	2–5 (2)	1–4 (3)	1–3 (2,3)	1–4 (2)	1–4 (3)	2–6 (2,3)	–	–
12	3–6 (4)	1	1	1	–	3–6 (3)	1–3 (1)	1,2 (1)	1,2 (1)	1,2(1)	3–5 (4)	–	–
13	3–5 (4)	–	16–23 (20)	9–13 (10)	6–10 (7,8)	6–11 (7)	7–10 (8)	7–11 (8)	7–12 (9)	7–12 (8,10)	8–11 (8)	–	–
14	2,3 (2)2	1,2 (2)	8–14 (11)	–	–	–	–	–	–	–	–	1	–
15	2–4 (3,4)	–	–	–	–	–	–	–	–	–	–	–	–