Myrsidea pachyramphi, Kolencik & Sychra & Papousek & Kuabara & Valim & Literak, 2018

Kolencik, Stanislav, Sychra, Oldrich, Papousek, Ivo, Kuabara, Kamila M. D., Valim, Michel P. & Literak, Ivan, 2018, New species and additional data on the chewing louse genus Myrsidea (Phthiraptera: Menoponidae) from wild Neotropical Passeriformes (Aves), Zootaxa 4418 (5), pp. 401-431 : 420-422

Myrsidea pachyramphi , new species

( Figs 28 , 35 , 45–46 )

Type host. Pachyramphus polychopterus (Vieillot, 1818) —white-winged becard.

Type locality. San Rafael National Park , Paraguay (26°30'S, 55°47'W).

Type material. Ex Pachyramphus polychopterus : holotype ♀, San Rafael National Park, Paraguay (26°30'S, 55°47'W), 8–15 August 2012, I. Literak ( MMBC) . Paratypes: 2♀, 3♂ with the same data as holotype ( MMBC) .

Diagnosis. Myrsidea pachyramphi n. sp. is morphologically similar to M. incerta ( Kellogg, 1896b) and M. pricei Clay, 1966 from members of the Turdidae , and to M. bessae Price, Johnson & Dalgleish, 2008 and M. alexanderi Kolencik, Sychra, Valan & Literak, 2016 from members of the Troglodytidae . Combining genetic and morphological difference, the closest species is M. incerta . However, females of M. pachyramphi can be distinguished from those of M. incerta by having (1) a larger number of setae on the femoral brush (18–22 vs 13– 17), and (2) larger dimensions, as follows: MW (0.42–0.43 vs 0.37) and AWIV (0.52–0.57 vs 0.50), while males differ by having a greater total number of setae on sternites IV–VII (85–96 vs 53–82). Further, females of M. pachyramphi , differ from those of M. pricei in (1) the shape of the first tergite (more concave in M. pricei ); and (2) the number of setae on tergites I–IV (total 28–38 in M. pachyramphi vs. 40–52 in M. pricei ). Males can be distinguished by the number of setae on tergites I–V (total 29–33 in M. pachyramphi vs. 39–53 in M. pricei ). Females of M. pachyramphi can be easily distinguished from those of M. bessae by the shape of the metanotum and first two tergites. Finally, M. pachyramphi differs from M. alexanderi in the number of setae on tergites I–VII: females with a total of 44–61 setae in M. pachyramphi vs. 74–90 in M. alexanderi ; males with a total of 38–43 vs. 63–71.

Description. Female (n = 3). As in Figs 28 and 45 . Hypopharyngeal sclerites fully developed. Length of dhs 10, 0.045–0.055; dhs 11, 0.090–0.105; ratio dhs 10/11, 0.50–0.52; ls5 0.04–0.05 long, latero-ventral fringe with 10–11 setae. Gula with 4–5 setae on each side. Pronotum with 6 setae on posterior margin and 3 short spiniform setae at each lateral corner. First tibia with 3 outer ventro-lateral and 4 dorso-lateral setae. Metanotum not enlarged, with 8–9 marginal setae; metasternal plate with 6–7 setae; metapleurites with 3–4 short strong spiniform setae. Femur III with 1 8–22 setae in ventral setal brush. Tergites I and II slightly convex. Abdominal segments with welldefined median gap in each row of tergal setae. Tergal setae: I, 6–8; II, 8–11; III, 8–10; IV, 6–9; V, 7–10; VI, 5–8; VII, 4–5; VIII, 4. Postspiracular setae very long on II, IV and VIII (0.45–0.51); long on I and VII (0.30–0.38); and short on III, V and VI (0.12–0.23). Inner posterior seta of last tergum not longer than anal fringe setae with length 0.06–0.08; length of short lateral marginal seta of last segment, 0.03–0.04. Pleural setae: I, 4–5; II, 6–8; III, 7–8; IV, 6–7; V, 5–6; VI–VII, 4–5; VIII, 3. Pleurite VIII with inner setae (0.07–0.09) twice as long as outer (0.04–0.05). Anterior margin of sternal plate II with a medial notch. Sternal setae: I, 0; II, 3–4 in each aster: s1, 0.05–0.06; s2, 0.04–0.05; s3, 0.03–0.04; s4, 0.02–0.03; with 13–16 marginal setae between asters, 3–4 medioanterior; III, 21–24; IV, 32; V, 33–35; VI, 27–30; VII, 10–11; VIII–IX, 8–10; and 10–12 setae on deeply serrated vulvar margin. Anal fringe formed by 40 dorsal and 35 ventral setae. Dimensions: TW, 0.45–0.46; POW, 0.35–0.36; HL, 0.31–0.32; PW, 0.27–0.28; MW, 0.42–0.43; AWIV, 0.52–0.57; ANW, 0.21–0.23; TL, 1.43–1.45.

Male (n = 3). As in Fig. 46 . Similar to female exept as follows: length of dhs 10, 0.045–0.050; dhs 11, 0.093– 0.100; ratio dhs 10/11, 0.48–0.50; ls5 0.05 long, latero-ventral fringe with 10–11 setae. Gula with 5 setae on each side. Pronotum with 6 setae on posterior margin and 3 short spiniform setae at each lateral corner. First tibia with 3 outer ventro-lateral and 4 dorso-lateral setae. Metanotum not enlarged with 6–7 marginal setae; metasternal plate with 6–7 setae; metapleurites with 3 short spiniform strong setae. Femur III with 13–17 setae in ventral setal brush. Abdominal segments with well-defined median gap in each row of tergal setae. Tergal setae: I, 4; II, 6–7; III, 6–8; IV, 7; V, 6–7; VI, 5–6; VII–VIII, 4; Postspiracular setae with the same pattern as in female but shorter. Length of inner posterior seta of last tergum, 0.03–0.05; short lateral marginal seta of last segment, 0.02. Pleural setae: I, 3–4; II, 4–5; III–IV, 5–6; V, 4–6; VI, 4–5; VII, 3–4; VIII, 2–3. Pleurite VIII with inner setae (0.06–0.07) as long as outer (0.02–0.03). Anterior margin of sternal plate II with a medial notch. Sternal setae: I, 0; II, 3–4 in each aster: s1, 0.05–0.06; s2, 0.03–0.04; s3, 0.03; s4, 0.02–0.03; with 8–10 marginal setae between asters, 2–4 medioanterior; III, 15–17; IV, 23–28; V, 27–29; VI, 22–25; VII, 13–14; VIII, 4; remainder of plate, 6–7; and with 3 setae posteriorly; with 8 internal anal setae. Genital sac sclerite as in Fig. 35 . Dimensions: TW, 0.41–0.43; POW, 0.32–0.33; HL, 0.28–0.30; PW, 0.25–0.26; MW, 0.35–0.36; AWIV, 0.42–0.44; GW, 0.10–0.11; GSL, 0.08; TL, 1.23–1.28.

Etymology. The species epithet is a noun in apposition derived from the generic name of the type host.

Remarks. This is the first record of chewing lice from Pachyramphus polychopterus . A portion of COI gene was sequenced from two specimens of M. pachyramphi from Paraguay (GenBank MF563534 – MF563535 ). Comparing our sequence with all known Myrsidea sequences, the closest were: (1) those of M. incerta (ex Catharus ustulatus (Nuttall, 1840) , GenBank FJ 171268 – FJ 171269 , and Catharus minimus (Lafresnaye, 1848) , GenBank FJ 171270 , family Turdidae ), with p-distances of 11.9–12.9%, (2) those of M. alexanderi (ex Pheugopedius maculipectus Lafresnaye, 1845 , GenBank MF563536 ), and M. bessae (ex Pheugopedius fasciatoventris Lafresnaye, 1845 , GenBank EU289214 , and Cantorchilus semibadius Salvin, 1870 , GenBank EU289213 family Troglodytidae ), with p-distances of 12.7–13.7%, and (3) that of M. pricei (ex Catharus guttatus (Pallas, 1811) , GenBank FJ 171273 ), with a p-distance of 13.7%. These divergences are close to the limit of accepted interspecific variation but, together with their morphological differences, we are confident that M. pachyramphi is a distinct species. Further molecular data are needed to clarify the phylogenetic relationships of these species within the species-complex and confirm their valid status as species or subspecies.

Figures

FIGURES 28–29. Dorso-ventral views of female thorax and abdomen: 28, Myrsidea pachyramphi n. sp.; 29, Myrsidea leptopogoni n. sp.

FIGURES 30–40. Male genital sac sclerites: 30–34, Myrsidea capeki n. sp.; 35, Myrsidea pachyramphi n. sp.; 36, Myrsidea contopi from Leptopogon amaurocephalus from Perú; 37, same but from Paraguay; 38–39, Myrsidea leptopogoni n. sp.; 40, Myrsidea pitangi from Pitangus sulphuratusfrom Paraguay.

FIGURES 45–48. Myrsidea pachyramphi n. sp.: 45, holotype female; 46, paratype male; Myrsidea leptopogoni n. sp.: 47, holotype female; 48, paratype male.