Melanoleuca graminicola Kühner & Maire, Bull.

Nawaz, Farzana, Jabeen, Sana & Khalid, Abdul Nasir, 2017, New and noteworthy Melanoleuca (Pluteaceae) from Pakistan, Phytotaxa 311 (2), pp. 175-184 : 180-182

Melanoleuca graminicola Kühner & Maire, Bull. trimest. Soc. mycol. Fr. 50: 18 (1934)

Figure 2 : E–F; Figure 4 : A–E; Figure 5 : B

Description:— Pileus 33 mm diam., plano-convex to flat at maturity, umbonate, umbo prominent, surface smooth, dry, margin smooth, slightly incurved, light brown (10YR5/6) at disc, dark gray (7.5YR3/2) towards the margin. Lamellae emarginate, toothed, crowded; edges undulate, white. lamellulae frequent, of different lengths. Stipe 37 × 5 mm, apex slightly wider up to 5.5 mm central, cylindrical, base broad up to 10 mm diam., longitudinally striate, dry, slightly pruinose, brown (2.5YR4/4) in the middle, white from the extreme base and apex. Basal mycelial mat absent.

Basidiospores [20/1/1] (5.8‒) 6‒8.2 (‒8.34) × (4‒) 4.6‒5.8 (‒6) μm, [avl × avw = 7.2 × 5 µm, Q = 1.44], ellipsoid, warty, thin-walled, hyaline in 5% KOH, amyloid warts, apiculus present. Basidia 20.8‒33 × 7.6‒11.3 μm, [avl × avw = 28.9 × 9 μm], clavate, 2‒4 spored, thin-walled, hyaline, sterigmata up to 2.9 μm long. Cheilocystidia 28–40 × 7.2–12 μm, [avl × avw = 33 × 9.4 µm], clavate, crystalline apex. Pleurocystidia absent. Pileipellis a cutis of hyphae 4–18.7 μm in diam. (av = 10 µm), thin-walled, septate, branched, hyaline. Stipitipellis composed of cylindrical hyphae 1.8–14.3 μm in diam. (av = 9.3 µm), hyaline. Clamp connections absent.

Habitat:— Dry temperate coniferous forests. Specimens examined: PAKISTAN. Khyber Pakhtunkhwa province: Swat district , Mashkun , 2500 m asl, on soil under C. deodara , 5 September 2013, Sana Jabeen and Abdul Nasir Khalid SJ52 ( LAH35059 !; GenBank KX908113 ) .

Figures

FIGURE 2. Basidiomata of Melanoleuca dirensis. A & B. LAH35160 C & D. LAH35159 E & F. M. graminicola LAH35059. Bars: A–D = 1.2 cm, E & F = 0.8 cm. Photographed by Sana Jabeen and Abdul Nasir Khalid.

FIGURE 4. Anatomy of Melanoleuca graminicola. A–E. LAH35059. A. Basidiospores; B. Cystidia; C. Basidia; D. Pileipellis; E. Stipitipellis. Bars: A = 4.2 µm; B = 8.6 µm, C = 8 µm; D = 11 & E = 12 µm. Drawing by Sana Jabeen.

FIGURE 5. Scanning electron microscope images of basidiospores. A. Melanoleuca dirensis (LAH35159); B. M. graminicola (LAH35059). Photographed by Abdullah Jan.