Cotylurus spp

Pyrka, Ewa, Kanarek, Gerard, Gabrysiak, Julia, Jezewski, Witold, Cichy, Anna, Stanicka, Anna, Elzbieta, Zbikowska, Zale, Grzegorz, sny & Hildebrand, Joanna, 2022, Life history strategies of Cotylurus spp. Szidat, 1928 (Trematoda, Strigeidae) in the molecular era - Evolutionary consequences and implications for taxonomy, International Journal for Parasitology: Parasites and Wildlife 18, pp. 201-211 : 206

3.4. Nucleotide pairwise comparison within Cotylurus spp

The overall interspecific divergence of analysed representatives of Cotylurus based on 28S rDNA alignment was 0.0%–3.13% (0–38 bp out of 1216 bp). However, when C. raabei was removed from this comparison, these values were significantly lower (0.0%–2.47%, 0–30 bp). Divergence within the “ C. strigeoides ” lineage was between 0.0% and 0.25%, while sequences of adult digeneans and larvae clustered within the “ C. cornutus sensu lato ” branch showed divergence in the range of 0.0%–1.23%.

Additionally, pairwise nucleotide comparisons of CO1 sequences within analysed Cotylurus spp . lineages are also provided. There were no intraspecific differences between the isolates of C. syrius A [lineage IX], while differentiation ranging between 1.02% and 1.70% was noted among sequences clustered within C. strigeoides (lineage VIII), and the overall interspecific divergence within sequences of the “ C. cornutus sensu lato ” group was 0%–7.8%. However, within the lineages of the “ C. cornutus s.l. ” clade, the maximum value of intraspecific nucleotide differences was as follows: for lineage II - 4.07%, for lineage III - 2.37%, and for lineages V and VII - 0.34%. For comparison, C. raabei and C. strigeoides differ by 8.14%, while C. raabei and C. cornutus differ by 9.83%.

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