Phaeoisaria annesophieae Hern.

Shi, Lin, Yang, Hao, Hyde, Kevin D., Wijayawardene, Nalin N., Wang, Gen-Nuo, Yu, Xian- Dong & Zhang, Huang, 2021, Freshwater Sordariomycetes: new species and new records in Pleurotheciaceae, Pleurotheciales, Phytotaxa 518 (2), pp. 143-166 : 158-160

Phaeoisaria annesophieae Hern. -Restr., Persoonia 39: 447 (2017)

Index Fungorum number: IF823031

Holotype: CBS H-23231, ex-type strain CBS 143235

FIGURE 6

Description: Saprobic on submerged wood. Sexual morph: Undetermined. Asexual morph: Colonies on natural substrata scattered, erect, rigid, brown to dark brown, composed of parallelly compact conidiophores. Conidiophores macronematous, synnematous, mid brown to dark brown, smooth, 197–456 μm long, 1–1.4 μm wide. Synnemata erect, rigid, smooth, velvety, dark brown, 10.5–15 μm (x = 12.6 μm, n = 10) wide, composed of parallelly compact conidiophores, conidiophores splaying out or divergent at the apical part. Conidiogenous cells polyblastic, terminal, integrated, later becoming intercalary, smooth, curved, cylindrical or subulate, sympodial extending, each with several denticulate conidiogenous loci, subhyaline to pale brown. Conidia cylindrical or ellipsoidal to obovoid, rounded at the apex, obtuse and tapering towards base, hyaline, aseptate, some with guttulae, smooth-walled, 4–6 × 2–3 μm (x = 5.2 × 2.4 μm, n = 10).

Cultural characteristics: Conidia germinated on PDA within 24 hours. Colonies grow on PDA attaining 12–14 mm diameter in 13 days at 25°C in the condition of 12h-dark and 12h-light, floccose, circular, white at first, becoming pale brown when aged, with smooth mycelium on the surface, in reverse brown to dark brown, with undulate margin.

Material examined: THAILAND. Nakhon Phanom Province, a small river of That Phanom , on dead submerged decaying wood of unidentified plants, 12 December 2018, Hao Yang, t13 ( MFLU 19-0531 ), living culture MFLUCC 19-0325 .

Notes: Phylogenetic analyses showed that the new isolate clustered with Phaeoisaria annesophieae (strain CBS 143235) with strong bootstrap support (100%/1.00, MLBS / MBPP, Figure 1 ). Phaeoisaria annesophieae was origially introduced from soil in Netherlands by Crous et al. (2017), with only morphological characters from culture. Our collection was from submerged decaying wood and fits well with the characters of Phaeoisaria ( Crous et al. 2017) . The two strains share similar characters in having ellipsoidal to obovoid conidia, but our collection has smaller conidia (4–6 × 2–3 μm vs. 4.5–9 × 2–3.5 μm) ( Crous et al. 2017). A detailed description of our collection on the substrate is provided. This is the first report of P. annesophieae from freshwater habitats and first record in Asia ( Thailand).

Figures

FIGURE 1. Backbone tree for Pleurotheciales generated from RAxML analyses based on a dataset of combined LSU, SSU, ITS and RPB2 sequence data. Bootstrap support values for maximum likelihood (the first value) equal to or greater than 70% and Bayesian posterior probabilities (the second value) equal to or greater than 0.95 are given above the nodes. The strain numbers are noted after the species names. Newly generated sequences are indicated in blue, new species are in red, and ex-type isolates are in bold. The tree is rooted with Conioscypha tenebrosa and C. minutispora. Strains known from freshwater habitats are marked as “ ”.

FIGURE 6. Phaeoisaria annesophieae (MFLU 19-0531). a, b Colonies on natural substrate. c, d Conidiophores. e, f Conidiogenous cells. g–n Conidia. o Germinating conidium on PDA. p, q Culture on PDA from above and reverse. Scale bars: c, d = 50 μm, e, o = 10 μm, f = 5 μm, g–n = 2 μm.