Carlosrosaea foliicola Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 85

Carlosrosaea foliicola Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828745. Fig. 7K .

Etymology: the specific epithet foliicola refers to the substrate origin of the type strain, leaves.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ovoid and ellipsoidal, 1.7– 4.0 × 2.5 –5.8 μm and single, budding is polar ( Fig. 7K ), a sediment is formed. After 1 mo at 17 °C, a part ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is white-cream, butyrous, smooth and glistening. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, maltose, cellobiose, trehalose, lactose, melibiose, raffinose, melezitose, soluble starch, D-xylose, L-arabinose, D-arabinose (delayed and weak), D-ribose, L-rhamnose (delayed and weak), D-glucosamine, ethanol, glycerol, erythritol, ribitol (delayed and weak), galactitol (delayed and weak), D-mannitol, D-glucitol, Methyl-α- Dglucoside, salicin, DL-lactate, succinate (delayed and weak), citrate (delayed and weak) and myo-inositol (weak) are assimilated as sole carbon sources. L-sorbose, inulin, methanol and hexadecane are not assimilated. Ammonium sulfate is assimilated as sole nitrogen sources. Potassium nitrate, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 30 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Ca. foliicola differs from the closely related species Ca. simaoensis in its ability to assimilate erythritol ( Table S1.5 ).

Typus: China, Wuzhishan mountain , Hainan province, obtained from a leaf of an unidentified plant, Nov. 2006, Q.-M. Wang (holotype CGMCC 2.3447 T preserved in a metabolically inactive state, ex-type CBS 15481 = WZS29.4).

Figures

Fig. 7. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Koc. haikouensis CGMCC 2.3443T; (C, D) Koc. ischaemi CGMCC 2.3565T; (E) Koc. nitrophila CGMCC 2.3465T; (F) G. pseudoamylolytica CGMCC 2.5809T; (G) Tr. shuangheensis CGMCC 2.5615T; (H) V. melezitolyticum CGMCC 2.3472T; (I) V. pseudopenaeus CGMCC 2.3165T; (J) V. europaea CGMCC 2.3099T; (K) Ca. follicola CGMCC 2.3447T; (L) Ca. simaoensis CGMCC 2.3580T; (M) Kwoni. ovata CGMCC 2.3439T; (N) Te. helanensis CGMCC 2.4450T; (O) Te. globosa CGMCC 2.5648T; (P) Te. korlaensis CGMCC 2.3835T. Bars = 10 μm.