Bensingtonia wuzhishanensis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 107

Bensingtonia wuzhishanensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828801. Fig. 14C, D .

Etymology: the specific epithet wuzhishanensis refers to the geographic origin of the type strain, Wuzhishan mountain, Hainan.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are cylindrical or fusiform, 3.4– 4.0 × 7.6– 10.0 μm and single, budding is polar ( Fig. 14C ), a sediment is present. On YM agar, after 1 mo at 17 °C, the streak culture is ivory to cream, mucoid, smooth and glistening. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are cylindrical, 2.9– 3.7 × 7.4– 10.0 μm ( Fig. 14D ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, maltose, cellobiose, trehalose, lactose (delayed and weak), melibiose, raffinose, melezitose, soluble starch, D-xylose, L-arabinose (delayed and weak), D-arabinose, D-ribose, L-rhamnose (weak), D-glucosamine (delayed and weak), ethanol, glycerol (weak), erythritol, ribitol, galactitol, D-mannitol, D-glucitol, Methyl-α- D-glucoside, salicin, DL-lactate, succinate (delayed and weak) and citrate (weak) are assimilated as sole carbon sources. L-sorbose, inulin, methanol, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, sodium nitrite (delayed and weak), L-lysine, ethylamine hydrochloride (delayed and weak) and cadaverine dihydrochloride (delayed and weak) are assimilated as sole nitrogen sources. Maximum growth temperature is 26– 27 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Be. wuzhishanensis differs from its closely related species, Be. pseudorectispora, Be. bomiensis, Be. naganoensis, Be. pseudonaganoensis and Be. rectispora , in its ability to assimilate D-ribose, ethanol and erythritol ( Table S1.18 ).

Typus: China, Wuzhishan mountain , Hainan province, obtained from a leaf of an unidentified plant, Nov. 2006, Q.-M. Wang (holotype CGMCC 2.3569 T preserved in a metabolically inactive state, ex-type CBS 15661 = WZS33.18).

Figures

Fig. 14. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Kon. rhododendri CGMCC 2.3463T; (C, D) Ben. wuzhishanensis CGMCC 2.3569T; (E) Ben. pseudorectispora CGMCC 2.5677T; (F) Ps. fusiformis CGMCC 2.5823T; (G, H) Ru. fanjingshanensis CGMCC 2.4542T; (I, J) Ru. bangxiensis CGMCC 2.3454T; (K, L) Ru. lunata CGMCC 2.4426T; (M, N) Boe. sterigmata CGMCC 2.4539T, (O) St. layueensis CGMCC 2.5817T; (P) Pse. motuoensis CGMCC 2.5816T. Bars = 10 μm.