Cunninghamella echinulate, ATCC

Eliwa, Duaa, Albadry, Mohamed A., Ibrahim, Abdel-Rahim S., Kabbash, Amal, Meepagala, Kumudini, Khan, Ikhlas A., El-Aasr, Mona & Ross, Samir A., 2021, Biotransformation of papaverine and in silico docking studies of the metabolites on human phosphodiesterase 10 a, Phytochemistry (112598) 183, pp. 1-12 : 7

3.6. Microbial transformation using Cunninghamella echinulate ATCC 1382

Biotransformation of papaverine using Cunninghamella echinulate ATCC 1382 afforded to one metabolite. The residue ( 900 mg) was dissolved in 20 ml of methanol/dichloromethane mixture (1:1) and adsorbed onto 900 mg Celite and dried. The adsorbed sample was placed onto a glass column (110 × 2.5 cm) packed with silica after making a slurry in dichloromethane. The column was gradiently eluted with dichloromethane 100% then dichloromethane: methanol (99.5:0.5, 99:1) and 20 ml fractions were collected. Fractions 50–75 were pooled together to give pure 4, as determined by TLC. The metabolite was obtained in the form of white powder ( 8 mg, R f 0.5 S 2).