Beauveria bassiana, (Bals.-Criv.) Vuill.

Eliwa, Duaa, Albadry, Mohamed A., Ibrahim, Abdel-Rahim S., Kabbash, Amal, Meepagala, Kumudini, Khan, Ikhlas A., El-Aasr, Mona & Ross, Samir A., 2021, Biotransformation of papaverine and in silico docking studies of the metabolites on human phosphodiesterase 10 a, Phytochemistry (112598) 183, pp. 1-12 : 9

3.7. Microbial transformation using Beauveria bassiana 22864

Biotransformation of papaverine using Beauveria bassiana 22864 afforded to two metabolites: 5 and 6. The residue ( 1 g) was dissolved in 30 ml of methanol/dichloromethane mixture (1:1) and adsorbed onto 1 g Celite and dried. The adsorbed sample was placed onto a glass column (110 × 2.5 cm) packed with silica after making a slurry in dichloromethane. The column was gradiently eluted with dichloromethane: methanol (99:1, 95:5,94:6) and 10 ml fractions were collected. Fractions 50–119 were pooled together to give mixture of the two metabolites ( 80 mg), as determined by TLC. The two metabolites were separated by preparative silica gel 60 PR-18 plates (10 × 20 cm, 250 μm) (methanol: water, 70:30). 5 mg of the sample added in each plate. The bands were located using UV lamp then scratched and extracted using 100% methanol then evaporated, yielding to pure 5 in the form of white powder ( 5 mg, R f 0.69 S 3) and pure 6 white powder ( 6 mg, R f 0.61 S 3).