Insect

Ozoliņa-Pole, Laura & Vilcāne, Jūlija, 2015, M O L E C U L A R I D E N T I F I C A T I O N A N D Characterization Of Synanthedon Tipuliformis Clerck From Black Currant Fields, Acta Biologica Universitatis Daugavpiliensis 15 (1), pp. 157-163 : 158-159

Insect sampling and preparation

Samples of S. typuliformis were collected in three distinct parts of Latvia, in Pūre (Western part, municipality of Tukums), Pārgauja (Northern part, municipality of Pārgauja) and Lutriņi (South-Western part, municipality of Saldus). Locations were chosen to be geographically distinct ( Fig. 1 ), the farthest distance was 162 km, from Pūre to Pārgauja, the nearest distance – 92 km. Plantations of black currants were 7-10 years old, shoots visually damaged by clearwing moth were collected. Shoots were cut along, larvae were removed from damage, and preserved in 96% alchocol at 5 °C until needed. In the case if the larvae were alive, they were put into exiccators covered with cotton cloth, together with some black currant stems and leaves, and

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Development Collection Gene Bank accession Location ID

stage date number mature imago were grown, to identify species. Afterwards mature imagoes were preserved in the same way, as larvae.

Figures

Fig. 1. Location of black currant plantations, where shoots damaged by S. typuliformis were collected.