Microbacterium proteolyticum BT

Kim, Ju-Young, Maeng, Soohyun, Park, Yuna, Lee, Sang Eun, Han, Joo Hyun, Cha, In-Tae & Kim, Ki-eun Lee and Myung Kyum, 2021, Twelve unrecorded UV-resistant bacterial species isolated in 2020, Journal of Species Research 10 (4), pp. 321-335 : 329

Description of Microbacterium proteolyticum BT 558

Cells are Gram-stain-positive, non-flagellated and rod-shaped. Colonies are yellow colored after 3 days of incubation on R2 A at 25℃. In the BIOLOG GEN III test, positive for dextrin, D-maltose, D-trehalose, D-cellobiolose, gentiobiose, sucrose, D-turanose, pH 6, β -D-glucoside, D-salicin, 1% NaCl, 4% NaCl, α -D-glucose, D-mannose, D-fructose, D-galactose, inosine, sodium lactate, D-mannitol, glycerol, gelatin, L-alanine, L-aspartic acid, L-glutamic acid, L-serine, pectin, D-gluconic acid, quinic acid, tetrazolium blue, pheylacetic acid, methyl pyruvate, L-lactic acid, α -glutaric acid, L-malic acid, nalidixic acid, lithium chloride, potassium tellurite, β -butryric acid, acetoacetic acid, propionic acid, acetic acid, formic acid, aztreonam and sodium butyrate; weak positive for stachyose, D-raffinose, N -glucosamine, β -mannosamin, N -galactosamin, neuraminic acid, D-arabitol, myo-inositol, D-gluose, D-fructose, D-aspartic acid, glyctl-L-proline, L-arginine, L-histidine, pyroglutamic acid, galacturonic acid, mucic acid, D-saccharic acid, tetrazolium violet, D-lactic acid, D-malic acid, Tween 40, γ -butryric acid, α -butryric acid, α -butyric acid and sodium bromate; but negative for pH 5, α -D-lactose, D-melibiose, 8% NaCl, 6-methyl-glucose, D-fucose, L-fucose, D-galactose, fusidic acid, D-serine, D-serine, troleandomycin, rifamycin SV, minocycline, lincomycin, guanidine HCl, Niaproof 4, galactonic lactone, D-glucuronic acid, glucuronamide, vancomycin, citric acid and succinic acid. In API 20NE, esculin hydrolysis, gelatin hydrolysis, β -galactosidase, D-glucose and D-mannitol were utilized; L-arabinose, D-mannose, D-maltose and potassium gluconate were weakly utilized; whereas reduction of nitrates (NO 3 -) to nitrite (NO 2 -), reduction of nitrates (NO 3 -) to nitrogen (N 2), indole production on tryptophan, glucose fermentation, arginine dihydrolase, urease, N -acetyl-D-glucosamine, capric acid, adipic acid, malic acid, trisodium citrate and phenylacetic acid were not utilized. In the API 32GN test, D-ribose, D-saccharose (sucrose), potassium 5-ketogluconate, salicin, Larabinose and potassium 2-ketogluconate were utilized; while L-rhamnose, N -acetyl-glucosamine, inositol, Dmaltose, itaconic acid, suberic acid, sodium malonate, sodium acetate, lactic acid, L-alanine, glycogen, 3-hydroxybenzoic acid, L-serine, D-mannitol, D-glucose, D-melibiose, D-fucose, D-sorbitol, propionic acid, capric acid, valeric acid, trisodium citrate, L-histidine, 3-hydroxybutyric acid, 4-hydroxybenzoic acid and L-proline were not utilized. Strain BT 558 (= NIBRBAC000506214) was isolated from a soil sample from Namhansanseong, Gyeonggi-do, Korea .