Euplotes moebiusi Kahl, 1932

Tirjaková, Eva, Botlíková, Simona & Vďačný, Peter, 2015, Checklist and distribution of ciliates from the family Euplotidae Ehrenberg, 1838 (Protista: Ciliophora: Spirotrichea) in Slovakia, Central Europe, Zootaxa 3920 (2), pp. 343-365 : 350-353

Euplotes moebiusi Kahl, 1932

( Figs 2 A–G; Table 3 )

This species was described from salt water ( Kahl 1932). Later it was recorded also from activated sludge and freshwaters where prefers polysaprobic or beta- to alfa-mesosaprobic levels (for review, see Foissner et al. 1991).

In Slovakia, E. moebiusi was found at several localities: in the River Danube near Sporná Sihoť ( Matis & Tirjaková 1995; unpubl. observ.); in the Morava river ( Baláži & Matis 1999, 2002; Baláži 2010); in streams of SE Slovakia during years 2011–2012 (unpubl. observ.); and in the sediments of the Váh river near the village of Lisková ( Tirjaková & Vďačný 2013a).

Characteristics Species a Method b Mean M SD SE CV Min Max n c. .....continued on the next page

aEAE— Euplotoides aediculatus , EAF— Euplotopsis affinis , EMO— Euplotes moebiusi , EMU – Euplotopsis muscicola , EPA— Euplotoides patella , EWO— Euplotoides woodruffi .

bPI—protargol impregnation, SN—silver nitrate impregnation.

c Size values for those species in which n <10 should be taken with caution due to the considerable variation in body length and width in euplotids. Problematic values are marked by an asterisk.

Figures

FIGURE 2. Euplotes moebiusi, Slovak specimens after protargol (A – E) and silver nitrate (F, G) impregnation. (A) Lateral view. (B – E) Ventral (B, C, E) and dorsal (D) views showing cirral pattern and nuclear apparatus. Arrowheads mark the two caudal cirri. (F) Ventral view of anterior body portion showing the irregular silverline system. (G) Dorsal view showing the double- eurystomus silverline pattern. AZM—adoral zone of membranelles, DK—dorsal kineties, FV—frontoventral cirri, MA—macronucleus, MC—left marginal cirri, PM—paroral membrane, TC—transverse cirri. Scale bars: 10 µm (F, G) and 20 µm (A – E).

Tables

TABLE 3. Morphometric characterization of six euplotid species recorded in the territory of Slovakia. Data are based on mounted specimens. CV—coefficient of variation in %, M—median, Max—maximum, Mean—arithmetic mean, Min—minimum, n — number of specimens examined, SD—standard deviation, SE—standard error.

Body, length (µm) EAE	PI	99.5	100.0	3.7	1.7	3.7	95.0	105.0	5*
EAF	SN	45.8	46.2	3.4	1.4	7.5	40.0	50.0	6*
EMO	PI	54.5	52.5	3.8	0.9	7.0	50.0	62.5	19
EMU	SN	66.8	66.2	6.1	0.9	9.2	55.0	80.0	50
EPA	SN	77.4	73.8	13.6	2.8	17.5	60.0	112.5	24
EWO	SN	168.4	166.0	6.4	2.0	3.8	142.0	198.0	10
Body, width (µm) EAE	PI	60.5	60.0	4.8	2.1	8.0	55.0	67.5	5*
EAF	SN	28.8	28.7	3.4	1.4	12.0	25.0	32.5	6*
EMO	PI	43.8	42.5	4.7	1.1	10.8	35.0	52.5	19
EMU	SN	55.6	55.0	9.3	1.3	16.7	40.0	77.5	50
EPA	SN	51.4	50.0	10.5	2.1	20.5	37.5	75.0	24
EWO	SN	105.6	102.0	3.4	1.1	3.2	90.0	130.0	10
Adoral zone of EAE membranelles, length EAF (µm) EMO	PI SN PI	62.5 31.7 36.3	62.5 30.0 37.5	– 2.9 2.1	– 1.7 0.5	– 9.1 5.8	62.0 30.0 32.5	63.0 35.0 40.0	2 3 19
EMU	SN	44.6	45.0	3.1	0.6	7.1	37.5	50.0	30
EPA	SN	48.2	47.5	6.7	2.5	14.0	40.0	60.0	7
EWO	SN	122.0	124.0	4.3	1.4	3.5	102.0	142.0	10
Adoral membranelles, EAE number EAF	PI SN	49.0 27.7	49.0 28.0	– –	– –	– –	48.0 25.0	50.0 30.0	2 3
EMO	PI	30.6	30.0	1.4	0.4	4.6	28.0	32.0	15
EMU	SN	31.5	31.0	3.1	0.6	10.0	26.0	40.0	25
EPA	SN	44.7	46.0	–	–	–	40.0	48.0	3
EWO	SN	56.3	57.0	1.4	0.4	2.5	50.0	62.0	10
Frontoventral cirri, EAE number EAF	PI SN	9.0 9.0	9.0 9.0	0.0 0.0	0.0 0.0	0.0 0.0	9.0 9.0	9.0 9.0	5 3
EMO	PI	10.0	10.0	0.0	0.0	0.0	10.0	10.0	19
EMU	SN	9.0	9.0	0.0	0.0	0.0	9.0	9.0	50
EPA	SN	9.0	9.0	0.0	0.0	0.0	9.0	9.0	7
EWO	SN	9.0	9.0	0.0	0.0	0.0	9.0	9.0	10
Transverse cirri, EAE number EAF	PI SN	5.0 5.0	5.0 5.0	0.0 0.0	0.0 0.0	0.0 0.0	5.0 5.0	5.0 5.0	5 3
EMO	PI	5.0	5.0	0.0	0.0	0.0	5.0	5.0	19
EMU	SN	5.0	5.0	0.0	0.0	0.0	5.0	5.0	50
EPA	SN	5.0	5.0	0.0	0.0	0.0	5.0	5.0	7
EWO	SN	5.0	5.0	0.0	0.0	0.0	5.0	5.0	10
Left marginal cirri, EAE number EAF	PI SN	2.0 2.0	2.0 2.0	0.0 0.0	0.0 0.0	0.0 0.0	2.0 2.0	2.0 4.0	5 3
EMO	PI	2.0	2.0	0.0	0.0	0.0	2.0	4.0	19
EMU	SN	2.0	2.0	0.0	0.0	0.0	2.0	4.0	50
EPA	SN	2.0	2.0	0.0	0.0	0.0	2.0	4.0	7
EWO	SN	2.0	2.0	0.0	0.0	0.0	2.0	4.0	10