Entoloma dirensis A. Izhar & Khalid

Izhar, Aiman, Usman, Muhammad, Kiran, Munazza & Khalid, Abdul Nasir, 2022, Two new species of Entoloma (Entolomataceae) from Khyber Pakhtunkhwa, Pakistan, Phytotaxa 574 (3), pp. 226-240 : 233

Entoloma dirensis A. Izhar & Khalid sp. nov. ( Figs. 4 & 5 )

MycoBank: MB843783

Etymology:—The specific epithet dirensis refers to the type locality “Dir”.

Diagnosis:— Entoloma dirensis is differentiated from the closely related E. catalaunicum by having smaller cheilocystidia (20.9 × 8.8 µm), broader elements of pileipellis (15–55 µm wide) and lack of caulocystidia.

“ Type:— PAKISTAN. Khyber Pakhtunkhwa Province: Dir Upper District, Kumrat , in groups on grassy spots under Cedrus deodara , 35.5369˚ N, 72.2193˚ E, 2232 m, 30 July 2019, A. N. Khalid KU -74, ( LAH 36947!; ITS: MZ 157271 ; LSU: MZ 157267 ).”

Description:— Pileus 1.5–3.2 cm in diam., hemispherical to plano-convex, depressed at center, deflexed then involute margin, margin entire, fibrillose radially striate pileus surface, densely fibrilose at centre, initially light brownish olive (XXX 19”) to Saccardo’s umber (XXIX 17”) pileus, dark dull violet-blue (XXIV 53*) near margin, dry, dull, hygrophanous. Lamellae marginate, sub-decurrent, ventricose, moderately distant, white to pale violet (XXXVII 61”) with blackish brown (XXXIX 1”’) margin, uneven, edges serrulate, lamellulae abundant, in 1–2 tiers. Stipe 4.5–5.5 × 0.4–0.7 cm, cylindrical, slightly swollen at base, hollow, Vanderpoel’s violet (XXXVI 55”) at apex, dark Tyrian blue (XXXIV 47”) towards base, fibrillose striate, finely pruinose at upper part, not shiny, dry, white (L III) base tomentose. Odor and taste not recorded.

Basidiospores [40/2/2], (7.3–)9.6–10.9(–11) × (6.5–)6.9–8(–8.5) µm, (avL × avW = 10 × 7 µm), Q = 1.1–1.3, avQ = 1.2, heterodiametrical, with 5–7 very pronounced angles, thick-walled, hyaline in KOH, inamyloid, mono- multi guttulate. Basidia 37–38.6 × 9–10 µm, (avL × avW = 38 × 9.5 µm), clavate, hyaline in KOH, frequently 4-spored, rarely 2-spored, guttulate, clamp connections absent. Lamella edge sterile, serrulatum-type, cheilocystidia abundant, (13–)14–21.8(–23) × (6.1–)6.8–11.7(–15) µm, (avL × avW = 20.9 × 8.8 µm), clavate to obovoid, thin-walled, hyaline, some cheilocystidia with brown intracellular pigment in KOH, septate, with basal cells, guttules present. Hymenophoral trama regular formed by cylindrical to inflated cells, 73 – 100 × 5 – 9 µm. Pileipellis a cutis of loose cylindrical hyphae, in transition to a trichoderm at centre where terminal elements are erect, else composed of parallel hyphae with cylindrical to clavate terminal cells, 15–55 × 6–17 µm, suprapellis formed with cells of 12–18 × 3–7 µm, a subpellis with septate hyphae 2.8–6.4 µm, avw = 4.5 µm, intracellar pigments as clumps, pale yellowish brown in water, brown in KOH, clamp connections absent. Stipitipellis a cutis of cylindrical hyphae composed of narrow cylindrical cells with narrowly clavate terminal cells, terminal cells relatively inflated, 15–27 µm, avw = 19 µm, hyaline in KOH, hyphae parallel, septate, clamp connections absent in all tissues. Caulocystidia absent.

Additional material examined: PAKISTAN. Khyber Pakhtunkhwa Province: Dir Upper District, Kumrat , in meadows under Cedrus deodara , 35.5369˚ N , 72.2193˚ E, 2232 m, 12 August 2018, M . Usman & A. N . Khalid KU - 75, ( LAH 36948!; ITS: MZ 157272 ; LSU: MZ 157268 ) .

Figures

FIGURE 4. A–C: Basidiomata of Entoloma dirensis; D: Basidiospores, E: Cheilocystidia, Scale Bars A–C = 1 cm, D, E = 10 µm. Microscopic photos by:Aiman Izhar.

FIGURE 5.A–E. Entoloma dirensis(Holotype).Elements of hymeniumdrawn as shownby lightmicroscopy.A: Basidia,B: Basidiospores; C: Cheilocystidia; D: Pileipellis; E: Stipitipellis; Scale Bars A–D = 10 µm, E = 20 µm. Drawings by:Aiman Izhar.