Eclipidrilus palustris ( Smith, 1900 )

Steven & Fend, V., 2005, A review of the genus Eclipidrilus (Annelida: Clitellata: Lumbriculidae), with description of a new species from western North America, Zootaxa 969, pp. 1-42 : 27-31

Eclipidrilus palustris ( Smith, 1900) ( Figures 15–16 )

MATERIAL EXAMINED: Alabama: Cleburne Co.: stream in Talladega National Forest, near Coleman Lake, collected by K. Fritz, 1 mature and 2 nearly mature, unmated worms, dissected. Florida: Polk Co.: “in a decayed stump in a marsh”, III.1897, collected by A. Hempel, from the collection of F. Smith; Paratypes, USNM 0 25509. 1 sagittally sectioned, 1 transversely sectioned, 1 dissected atrium and spermatheca. Columbia Co.: Suwannee River at SR6, near Benton, 6.XI.2002, collected by J. Epler. 2 dissected. Hamilton Co.: Roaring Creek, 23.X.1996, collected by J. Epler. 1 dissected. Hogan’s Branch, north of White Springs, 25.X.1997, collected by J. Epler. 2 dissected. Orange Co.: Rock Springs Run, 1/ 2 km upstream of Wekiwa Springs, in shallow, sandy riffle, 3.XII.2002, collected by S. Fend. 3 dissected, 1 transversely sectioned, 1 sagittally sectioned. Small tributary to Rock Springs Run, near source, in leaf deposits, 3.III.2005, collected by S. Fend. 12 dissected. Tootoosahatchee Creek near Christmas, in shallow, sandy riffle, 6.XII.2002, collected by S. Fend. 4 dissected, 1 sectioned, all partially mature; 1 sectioned, nearly mature.

Supplementary description

Pharynx II–IV; much thinner ventrally than dorsally or laterally. Anterior segments with a single pair of highly convoluted commissural blood vessels. Each segment posterior to about X with two pairs of variably branched lateral vessels; they are highly branched in posterior segments. In middle segments, at least, one branch of the lateral vessels joins the perivisceral sinus, but others end in the coelom. Nephridia paired, single or absent on 6/7; the next on or posterior to 12/13.

Ventral chaetal bundles in IX and X slightly medial to those in other segments; ventral chaetae in X often smaller than those in adjacent segments. Male pores distinctly medial to chaetae in X, and spermathecal pores slightly medial to ventral chaetae in IX ( Fig. 15 A). Female pores in line with ventral chaetae at 11/12. Mature and partially mature worms with testes in X only. Anterior male funnels (on 9/10) smaller than posterior male funnels; vasa deferentia to 16 µm in diameter.

Internal pads of cells up to 150 µm thick cover much of the ventral body wall of X, anterior to male pores ( Fig. 16 A). In some specimens the ventral pads appear glandular, interspersed with muscle fibers, but in others they appear predominantly muscular. Anteriorly, some muscle fibers coalesce into a band extending to dorsolateral wall of body near 9/10 ( Fig. 16 A). Posteriorly, some muscle fibers join 10/11.

Each male pore within a shallow fold of thin epidermis subtending a short (60–70 µm), conical papilla, which forms a sheath surrounding the penis when protruded ( Figs. 15 B, 16B). Ental to the papillae, deep penial sacs typically extend posteriorly into XIII. The penial sacs are not greatly contracted when the penes are protruded: the sacs were the same length (1130 µm) in one specimen with one penis extended 850 µm beyond body wall and the other retracted; in another worm one sac was 1040 µm long, with the penis extending 980 µm. In some specimens the sacs were much shorter (about 600 µm, extending into XI), with retracted penes. Each penial sac surrounded by a very thin, transversecircular inner muscle layer (often indistinct), and a 15–50 µm longitudinal to somewhat spiral outer layer ( Fig. 16 C; see also Brinkhurst [1998, Fig. 2 C–D]). Ental end of sac bulblike, connected to a muscle band which usually joins the dorsal body wall musculature in XIV. Sac epithelium continuous with outer wall of the penis ( Brinkhurst [1998, Fig. 3 A]). Outer and inner epithelial layers of penis joined by sparse, oblique fibers of apparent muscle tissue, particularly in ental half ( Brinkhurst [1998 Fig. 2 D, 3A]). Outer wall of penis is a thin layer of flattened cells ( Brinkhurst [1998, Fig. 2 D]). Lumen of penis is variably twisted in ectal part ( Fig. 15 B). Penes may be folded or coiled within the sacs when retracted. Penial structures of worms from other sites may be much smaller than those from the type locality, but morphology otherwise similar.

Inner epithelium of penis continuous with epithelium of atrial duct. Entally, the atrial duct widens into the ampulla; ampulla has thin (about 3 µm) epithelium, surrounded by two muscle layers ( Fig. 16 F; see also Brinkhurst [1998 Fig. 2 C]). Inner atrial muscle layer thin (2–7 µm) and transverse­circular; outer longitudinal­spiral layer thick: about 80 µm in the type series; 36–100 µm in worms from other sites, with fibers arranged at 30–60º from longitudinal axis of ampulla ( Fig. 16 G). Ratio of maximum lumen width to atrial muscle tube diameter about 0.3 in 3 specimens from the type locality. In most Rock Springs Run specimens, the ratio varied from 0.27–0.55 (median 0.37). One exceptional specimen from that site had thin­walled, folded atrial ampullae (27 µm muscle layer and ratio 0.76); the small prostates and thick pad anterior to the male pores resembled other members of the population. Outer muscle layer fibers may be nearly parallel to longitudinal axis of ampulla in ectal part ( Fig. 16 H). Prostatic cells in small, petiolate clusters 50–120 µm long cover most of ampulla ( Fig. 16 F,G).

Spermathecal ducts terminate in narrow vestibules, 100–250 µm deep, filled with irregular, transverse lamellae composed of elongate epithelial cells ( Fig. 16 E). Epithelium of spermathecal ampulla thin (14–24 µm) in about the ectal 1/3–1/4; most sperm is in this area, unordered but densely packed. Remainder of ampulla with thick epithelium (about 35 µm), having basal nuclei ( Brinkhurst [1998, Fig. 2 A]).

In partially mature specimens, penial sacs extend only into XI, and retractor muscles appear to join the body wall musculature in XII. Penes conical, 1/3 to 2/3 the length of penial sacs ( Figs. 15 C, 16D), appearing as simple folds of sac lining and containing attenuated ectal ends of atrial ducts. Outer penial folds and glandular pads not present, but a mass of cells extends forward from base of penial structure at male pore. Atrial ampulla weakly differentiated from duct, with thick epithelium (ca. 16 µm), a thin layer of muscle, and an outer layer of loosely packed cells, some of which are in weak clusters.

In a nearly mature specimen, penial structures extend to 12/13, the retractor muscle to 13/14, and elongate penes extend to near the male pore. The small epidermal fold is partially formed; the pads of cells anterior to the male pores have thin cell extensions extending through the body wall musculature to the epidermis. Spermathecal vestibule similar to that of mature worm, but ampulla small, ovoid, with cuboidal epithelium.

Remarks

The elongate, highly modified penes of mature worms develop from simple extensions of the atrial ducts, contained within folds in the penial sacs. The penes of partially developed E. palustris resemble fully developed penes of some other lumbriculids, such as Rhynchelmis rostrata Eisen (cf. Fend and Brinkhurst [2000, Fig. 57]). The highly modified cell types of the mature worms develop gradually as the penes lengthen within the sacs. The small epidermal folds, which resemble the penial sacs of some other lumbriculids, appear late in the development.

The length and diameter of the penial structure in mature worms is quite variable, but does not appear strongly related to penial extension. Therefore, the extensive musculature surrounding the sac, including the ental “retractors” (or “retainers”, as suggested by Brinkhurst [1998]), appears to provide structural rigidity rather than directly extending the penis by contracting the sac. Protrusion thus appears to result at least in part from lengthening the penis itself. Brinkhurst (1998) suggested that the E. ( Premnodrilus ) penis is everted during extension, but the observation that the sinuate lumen straightens during extension suggests lengthening of the surrounding tissue. A possible mechanism would be contraction of the thin muscle strands within the penis, which may increase turgor as the expanded base is contracted. The “cuticularized penis sheath” mentioned by Wassell (1984) was not prominent or rigid in material examined in the present study, and penes were usually somewhat coiled or even folded back within the sacs in this species as well as in E. daneus . The small epidermal folds were often seen in an everted state, with the short papillae visible externally ( Fig. 15 B); this may be controlled by action of the retractor muscles.

Figures

FIGURE 2. Eclipidrilus pacificus (A – C, typical specimens, Hoh River, Washington), (D, typical specimen, Calawah River, Washington), (E, Variant 2, Warren Slough, Columbia River). A—chaetal bundles from two different worms: left, a ventral bundle in IX; right, a dorsal bundle in VI. Blateral view of segments I – V, from a cleared whole mount. C—lateral view, one of the lateral blood vessel pairs in LXXV. D—lateral view of a lateral blood vessel pair in LXVII. E—dorsal view of both lateral vessel pairs in LX. Scale bars 100 µm (A), 400 µm (B), 200 µm (C – E).

FIGURE 3. Reproductive segments of E. pacificus (A, typical specimen, Hoh River, Washington), (B, Variant 2, Knappa Slough, Columbia River). A—ventrolateral view, showing spermathecae and atrium (vasa deferentia and anterior funnels not shown), from a whole mount. B—ventral view (schematic) of a whole – mounted worm with three spermathecal pores, showing positions of spermathecae and atrium. Abbreviations: aa—atrial ampulla; ad—atrial duct; mf—posterior male funnel; mp—male pore; ov—ovary; sa—spermathecal ampulla; sp—spermathecal pore; ss—sperm sac; te—testis. Scale bars 500 µm.

FIGURE 15. Eclipidrilus palustris (A, Hogan’s Branch, Florida), (B, Roaring Creek, Florida), (C, Tootoosahatchee Creek, Florida). A—ventral view of a highly contracted specimen, showing protruded penes. B—sagittal view of male pore with protruded penis. C—atrium and penial structure of a partially mature worm, sagittal view. Abbreviations: aa—atrial ampulla; ef—epidermal fold; pe—penis; pp—penial papilla; ps—penial sac. Scale bars 1 mm (A), 100 µm (B – C).

FIGURE 16. Eclipidrilus palustris (A ­ B, G­H, from the type series, Polk Co., Florida), (C, E­F, Rock Springs Run, Florida), (D, Tootoosahatchee Creek, Florida). A—sagittal section of X, showing ventral pad and associated muscle. B—male pore, showing epidermal fold, with part of penis protruding; sagittal section. C—transverse section of penial structure. D—ental end of penial structure; sagittal section of a partially mature worm. E—spermathecal vestibule, from a transverse section. F—atrial ampulla, transverse section showing muscle layers and small prostate glands. Gental part of atrial ampulla, with outer muscle layer diagonal to the long axis; sagittal section. Hectal part of atrial ampulla, with outer muscle parallel to long axis. Abbreviations: ad—atrial duct; ef—epidermal fold; lm—longitudinal­diagonal muscle; mu—muscle; pe—penis; pp—penial papilla; ps—penial sac; tm—transverse­circular muscle. Scale bars 50 µm.