Indalia predotae ( Schawerda, 1927 ) Macià & Ylla & Gastón & Huertas & Bau, 2022

Macià, Ramon, Ylla, Josep, Gastón, Javier, Huertas, Manuel & Bau, Josep, 2022, The species of Eilema Hübner, [1819] sensu lato present in Europe and North Africa (Lepidoptera: Erebidae: Arctiinae: Lithosiini), Zootaxa 5191 (1), pp. 1-87 : 26-27

Indalia predotae ( Schawerda, 1927) comb. nov.

Original combination: Lithosia predotae Schawerda, 1927 . Zeitschrift des Österreichischen Entomologen-Vereines Wien 12: 37.

T. L.: Albarracín , (Aragón), Spain.

Material examined. SPAIN: HUESCA: 19 ♂ and 15 ♀, Tozal de la Mora, Villanueva de Sigena , 8.viii.1997, 3.viii.2002, 26.iv.2003, 31.v.2004, 10.ix.2005, 27.v.2006, 20.vi.2015, R. Macià & J. Ylla leg. ; 5 ♂ and 2 ♀, Barranco de Valcuerna , Candasnos, 295 m, 10.v.1993, 11.ix.2007, R. Macià & J. Ylla leg. ; 3 ♂, Ontiñena , 220 m, 30.viii.2008, R. Macià & J. Ylla leg. ; 4 ♂, Ontiñena , 215 m, 13.v.2015, J. Gastón leg. ; 2 ♂, Serreta Negra , Candasnos, 270 m, 3.x.2016, J. Gastón leg. ; TERUEL: 15 ♂ and 12 ♀, Camino rural de Moscardón a Royuela, Moscardón, 1446 m, 30TXK26, 4.ix.2013, R. Macià leg. ; 16 ♂ and 9 ♀ Valle de Valdevecar , Albarracín, 1125 m, 30TXK37, 15.v.1993, 27.viii.1995, 1.viii.1999, 3.ix.2013, 3.viii.2017, 11.viii.2018, R. Macià leg. ; 14 ♂ and 11 ♀, Camino Rural , Calomarde, 1550 m, 30TXK26, 2.viii.2017, R. Macià leg. ; ZARAGOZA: 6 ♂, Torralba de los Frailes, 1050 m, 27.vii.1999, J. Gastón leg. ; 15 ♂ and 8 ♀, Montes de la Retuerta, El Ciervo , 310 m, 30TYL39, 21.ix.2003, 24.ix.2005, 17.ix.2006, 19.ix.2014, R. Macià & J. Ylla leg. ; 18 ♂ and 14 ♀, Val de Boquero el Ciego, La Retuerta, 362 m, 30TYL29, 11.vi.2013, R. Macià leg.

Diagnosis. The grey-brownish wing tonality and the fringe between light yellow and ochre yellow make this species unmistakable. Indalia predotae is notably sexually dimorphic: the females are smaller, much lighter yellowbrown, and the wings are proportionally smaller in relation to the rest of the body.

Re-description. Imago ( Figs. 17–18 ). Average wingspan males 23.4 mm (n=10; 20–27 mm); average wingspan females 19.1 mm (n=10; 17–21 mm).

Genitalia ( Fig. 57 ). Male genitalia: uncus cylindrical, slender, elongated, not flattened, sclerotised, with a pointed tip; valvae ovoid with rounded cucullus, sacculus wide and sclerotised, saccular process weakly sclerotised and slightly curved towards the cucullus. Aedeagus with three small, almost equal cornuti, two thick and coarse and the third smaller and sharper. Female genitalia: anal papillae small, posterior apophysis long, ostium and ductus bursae membranous; corpus bursa ovoid with one signum in its lower part and a weakly sclerotised plaque with diffuse edges in the place where the wart is located in other species. The appendix bursae prior to the ductus seminalis is thin and not very prominent.

Immature stages ( Fig. 82 ). The last instar larva is 17 mm long. Cephalic capsule completely black. Verrucae light brown (the L1 wart is missing or barely noticeable), all with translucent and black setae. Dorsal line broad at first and then narrow, broken, dark brown; broad light brown band in the area of D1 and D2 and then until SD 1 dark brown. Lateral sites to L3 light brown with various brown sinusoid spots. Pupa stylized, brown and smooth, with dark spots on the dorsal area; back of metanotum and segments 1 to 8, very small circular depressions; tip of abdomen rounded, without cremaster. In pupa of male, the antennae and proboscis reach the end of the wings, in the females they are shorter. The cocoon under lichens, bark and roks, with the silk very close to the body.

Molecular data. This species forms a clade with strong BI support (PP=1) and moderate within-group distance (0.47% ± 0.18SE). RESL Cluster Analysis returned a single cluster and all sequences coincided with a published BIN (BOLD: ACD0670). Like its close neighbour Indalia marcida, COI distances with other members of the genus are quite similar, albeit with lower values (TN-dist between 5.13% and 6.54%, average 5.42%) than those found for the latter (6.44% –7.97%; average 7.31%).

Biology. Bivoltine, with a first generation in May, June and July, followed by a second one in August, September and October. Xerothermic biotopes are favoured, with abundant steppe vegetation, and clearings in dry and sunny wooded areas among junipers and pine forests. Colonies are found up to 1200–1500 m above sea level. Adults are active both by day and at night, and are attracted by artificial light. The larvae feed on lichens, accepting dry lettuce leaves in captivity ( Ylla et al. 2010), and also prepared artificial diet.

Distribution ( Fig. 103 ). Iberian endemic. Known mostly from different localities in northeastern Spain, but absent from the entire Pyrenean area. The following are some examples of classic biotopes: Monegros and areas bordering the Ebro Valley, Iberian System (Albarracín and neighbouring areas) and La Vid (Burgos). Isolated colonies are known in Araba, Valladolid and Badajoz. Also known from southern and central Portugal (Teodoro Monteiro, 1975; Corley et al., 2000; Pires & Corley, 2007). Recently reported in Catalonia, Erms d´Aitona (Lleida), Guzmán (2015). Not known from the Balearic Islands.

The uniola group

Indalia uniola (Rambur, [1866]) comb. nov.

Indalia albicosta ( Rogenhofer, 1894) comb. nov.

Indalia interposita ( Rothschild, 1914) comb. nov.

It comprises three South European species: Indalia albicosta , endemic from the Canary Isles and also known from a few western Andalusian localities; Indalia uniola and Indalia interposita , which are spread along the North of Africa and reach southern Europe at different extent: Indalia uniola colonizes the Iberian Peninsula and south-east France, whereas Indalia interposita is restricted to the arid and desertic biotopes of south-east Spain. Genetically, the three species form a clade with moderately high Bayesian support (BI=0.77, Fig. 119 ) which is confirmed and strengthened in the extended analysis (BI=0.88, Fig. 120 ), and presents similarly low distances for both mitochondrial (aver. TN-dist=5.65%) and nuclear (2.08%) markers. Despite their genetic similarity, they show some phenotypic features that allow a fast and reliable morphological identification.

Figures

FIGURE 57. Indalia predotae male and female genitalia, gen. prep. 7357JG, Valdevacer, Albarracín, Teruel (Spain); 7335JG, Valdevacer, Albarracín, Teruel (Spain).

FIGURE 82. Immature stages Indalia predotae (picture MH).

FIGURE 119. Radial representation of the unrooted phylogenetic tree obtained by Bayesian Inference. The combined data from the mitochondrial marker COI and the nuclear markers ArgK and DDX23 have been run in three different partitions in the BEAST2 software. Posterior probability values are displayed beside the nodes. For clarity, the branches under the species or subspecies nodes have been collapsed indicating the number of specimens for each taxon. Font colour indicates distinct groups as discussed in the text: Green: Eilema genus; Light blue: Manulea genus; Dark blue: lutarella group from Indalia genus; Yellow: uniola group from Indalia genus; Orange: predotae group from Indalia genus; Red: Nyea genus; Violet: Setema genus; Black: remaining species under study and outgroup species (Nudaria mundana).

FIGURE 120. Phylogenetic tree representation obtained by Bayesian Inference of the extended dataset including the COI Barcode sequences published in BOLD as Eilema or Manulea genus and the sequences obtained in this study. The combined data from the mitochondrial marker COI and the nuclear markers ArgK and DDX23 have been run in three different partitions in BEAST2. Posterior probability values are displayed beside the nodes. For clarity, the branches under the species or subspecies nodes have been collapsed indicating the number of specimens for each taxon next to the countries of collection (AU: Austria; CRO: Croatia; CYP: Cyprus; CZR: Czech Republic; DE: Denmark; FI: Finland; Fr: France; GE: Germany; GR: Greece; IT: Italy; MAC: Macedonia; NL: Netherlands; NOR: Norway; PAK: Pakistan; RU: Russia; SP: Spain; UK: United Kingdom). Branches in grey color and font correspond to clades not reviewed in the current work. In all other cases, font colour indicates distinct clades as discussed in the text: Green: Eilema genus; Light blue: Manulea genus; Dark blue: lutarella group from Indalia genus; Yellow: uniola group from Indalia genus; Orange: predotae group from Indalia genus; Red: Nyea genus; Violet: Setema genus; Black: remaining species under study and outgroup species (Nudaria mundana).

FIGURES 17–24. 17. Indalia predotae (Schawerda, 1927). Male: Camino rural de Moscardón a Royuela, Moscardón (Teruel) Spain, 1446 m, 30TXK26, 4.ix.2013, R. Macià leg.; 18. Female: Val de Boquero el Ciego, La Retuerta (Zaragoza) Spain, 362 m, 30TYL29, 11.vi.2013, R. Macià leg.; 19. Indalia albicosta (Rogenhofer, 1894). Male: Los Canarios, La Palma (Canarias) Spain, 800 m, 21.xii.2003, W. Bogi leg.; 20. Female: Los Canarios, La Palma (Canarias) Spain, 800 m, 20.vii.1896, W. Bogi leg.; 21. Indalia interposita (Rothschild, 1914). Male: Rambla Granatilla, Sopalmo (Almería) Spain, 15 m, 20.iv.2005, R. Macià & J. Ylla leg.; 22. Female: Rambla de la Sierra, Tabernas (Almería) Spain, 452 m, 30SWF59, 16.v.2013, R. Macià leg.; 23. Indalia uniola (Rambur, [1866]. Male: Pozo del Correo, Moscardón (Teruel) Spain, 1484 m, 30TXK25, 5.ix.2012, R. Macià leg.; 24. Female: Pozo del Correo, Moscardón (Teruel) Spain, 1484 m, 30TXK25, 5.ix.2012, R. Macià leg.

FIGURES 95–109. 95. Eilema caniola caniola; 96. Eilema caniola torsteni; 97. Manulea complana; 98. Manulea iberica; 99. Manulea pseudocomplana; 100. Manulea palliatella; 101. Manulea costalis; 102. Indalia marcida; 103. Indalia predotae; 104. Indalia albicosta; 105. Indalia interposita; 106. Indalia uniola; 107. Indalia lutarella lutarella; 108. Indalia lutarella luqueti; 109. Indalia pygmaeola pygmaeola.