Nycticeinops schlieffenii (Peters, 1859)

Volleth, Marianne, Mayer, Frieder, Heller, Klaus-Gerhard, Müller, Stefan & Fahr, Jakob, 2023, Karyotype comparison of five African Vespertilionini species with comments on phylogenetic relationships and proposal of a new subtribe, Acta Chiropterologica 25 (1), pp. 35-52 : 44

Nycticeinops schlieffenii (Peters, 1859)

The karyotype of N. schlieffenii consisted of nine meta- to submetacentric and seven acrocentric autosomal pairs (2n = 34 and FNa = 50). The X chromosomes were medium-sized metacentrics with a G-banding pattern characteristic for vespertilionid state II. In addition to the ancestral four bi-armed pairs, 1/2, 3/4, 5/6 and 16/17, derived Robertsonian fusion chromosomes 7/11, 8/12, 9/13, 10/14 and 21/22 were observed ( Fig. 11 ).

The SC close to the centromere of the acro-centric chromosome 15 was confirmed by AgNOR staining as nucleolus organizer region. The mean number of active NORs per cell was 1.9 (16 cell scored). Apart from the centromeres, C-positive heterochromatin was present in the proximal part of one of the two smallest acrocentric pairs, homologous to MMY24 or MMY25 ( Fig. 6C ). The chromosomes 1/2, 11, 12, and 15 were present in vespertilionid state II.

Figures

FIG. 11. G-banded karyotype of a female N. schlieffenii, 2n = 34. Homology to MMY validated with painting probes from MMY, Tree shrew or black lemur is indicated by bold numbers. The remaining pairs were identified by G-band comparison with other vespertilionid species

FIG. 6. C-banded metaphase spreads of (A) P. brunnea, (B) N. happoldorum, and (C) N. schlieffenii. X and Y chromosomes and heterochromatic segments of interest are indicated, numbers refer to MMY homologies. In (B), arrows point to those heterochromatic segments which showed differences between the homologs and arrowheads point to C-positive segments on pairs NHA11 and NHA13. For further explanation see main text