Pythonides latemarginatus Grishin, 2023

Zhang, Jing, Cong, Qian & Grishin, Nick V., 2023, Supplementary Materials and Appendix, Insecta Mundi 2023 (26), pp. 1-115 : 18-19

Pythonides latemarginatus Grishin , new species

https://zoobank.org/ 7434EACB-635C-435F-A365-2932F2749590

( Fig. 2 part, 37–38, 250–251)

Definition and diagnosis. Specimens from Panama identified as Pythonides jovianus amaryllis Staudinger, 1876 ( type locality in Panama and Colombia) are sister to all Pythonides jovianus (Stoll, 1782) and are genetically differentiated from it ( Fig. 2): e.g., their COI barcodes differ by 3.5% (24 bp) from P. j. amaryllis, and therefore they represent a new species. This new species keys to P. j. amaryllis (E.41.3(a)) in Evans (1953) and differs from it by a broader brown marginal area on the hindwing and correspondingly smaller blue patch, but more extensive forewing postdiscal blue spotting, in particular having a blue streak by the inner margin and two prominent blue streaks near forewing tornus beneath ( Fig. 37–38). This species is not cryptic and is diagnosed reliably by phenotype. In DNA, a combination of the following base pairs is diagnostic in the nuclear genome: aly 2178.10.1:C78T, aly 2178.10.1:C96T, aly363.24.5:G81A, aly522.12.2:G39A, aly9588.7.2:A27G, and COI barcode: T49A, T268C, T385C, T457C, T499C.

Barcode sequence of the holotype. Sample NVG-18018B04, GenBank OR837638, 658 base pairs: AACTTTATATTTTATTTTTGGAATTTGAGCTGGAATAGTAGGAACATCATTAAGTTTATTAATTCGAACTGAATTAGGTAATCCCGGTTCATTAATT GGAGATGATCAAATTTATAATACTATTGTAACTGCTCATGCTTTTATTATAATTTTCTTTATAGTAATACCAATTATAATTGGAGGATTTGGAAATT GACTAGTACCCCTTATACTAGGAGCTCCTGATATAGCATTCCCTCGAATAAATAATATAAGTTTTTGATTACTCCCCCCTTCTCTTACATTATTAAT TTCAAGAAGTATTGTTGAAAATGGAACTGGAACAGGATGAACAGTATACCCCCCTCTTTCCTCTAATATTGCTCATCAAGGATCTTCTGTTGACTTA

GCTATTTTTTCCTTACACCTAGCTGGTATTTCATCAATTTTAGGAGCTATTAATTTTATTACTACAATCATTAATATACGAATTAGAAATTTATCAT TTGATCAAATACCCTTATTTGTTTGAGCTGTAGGAATTACAGCATTATTATTATTATTATCATTACCTGTTTTAGCCGGAGCTATTACCATATTATT AACTGACCGAAATTTAAATACATCATTTTTTGATCCAGCTGGTGGAGGAGATCCAATTTTATATCAACATTTATTT

Type material. Holotype: ♂ deposited in the National Museum of Natural History , Smithsonian Institution,

Washington, DC, USA ( USNM), illustrated in Fig. 37–38, bears the following four rectangular labels, three white: [ PANAMA: Panama | Cerro Campana 2500′ | 8°41′N 79°55′W | August 1970 | leg. G.B.Small], [DNA sample ID: | NVG-18018B04 | c/o Nick V. Grishin], [USNMENT | {QR Code} | 01450934], and one red [ HOLOTYPE ♂ | Pythonides | latemarginatus Grishin ]. Paratype: 1♂ NVG-18018B03 USNMENT_01450933 Panama: Darien, Cana, 750 m, 15-Jun-1983, G. B. Small leg. [ USNM].

Type locality. Panama: Panama Province, Cerro Campana, elevation 2500 ft, GPS 8.6833, −79.9167.

Etymology. The name is Latin for the broad (dark) margin and is a masculine adjective. Distribution. Panama.