taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
03CD521DFFED8178FF1B4B45FEAF83B3.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14386283/files/figure.png	https://doi.org/10.5281/zenodo.14386283	FIGURE 5. Primer annealing sites and multiplex AS-PCR results for samples of Simulium reptans s.l. from the Si16 sample (Novosibirsk). A–Annealing site of a primer specific for S. reptantoides; consensus sequences of molecular forms are given, as well as the sequence of a unique individual that we studied. B–Annealing site of a primer specific for S. reptans. C–results of electrophoresis of AS-PCR products from the Si16 sample, lane No. 4 corresponds to the unique sample Si16-4, which gives two PCR products, for the remaining specimens only the PCR product with the size predicted for S. reptans is visible (579 bp). Lane on the right (No. 11)–molecular weight marker 100bp+1.5+3. For A and B, vertical lines indicate complementary pairs, and the arrow indicates the order of nucleotides in the primer.	FIGURE 5. Primer annealing sites and multiplex AS-PCR results for samples of Simulium reptans s.l. from the Si16 sample (Novosibirsk). A–Annealing site of a primer specific for S. reptantoides; consensus sequences of molecular forms are given, as well as the sequence of a unique individual that we studied. B–Annealing site of a primer specific for S. reptans. C–results of electrophoresis of AS-PCR products from the Si16 sample, lane No. 4 corresponds to the unique sample Si16-4, which gives two PCR products, for the remaining specimens only the PCR product with the size predicted for S. reptans is visible (579 bp). Lane on the right (No. 11)–molecular weight marker 100bp+1.5+3. For A and B, vertical lines indicate complementary pairs, and the arrow indicates the order of nucleotides in the primer.	2024-12-04	Vaulin, Oleg V.;Petrozhitskaya, Liudmila V.;Novgorodova, Tatiana A.		Zenodo	biologists	Vaulin, Oleg V.;Petrozhitskaya, Liudmila V.;Novgorodova, Tatiana A.			
03CD521DFFED8178FF1B4B45FEAF83B3.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14386285/files/figure.png	https://doi.org/10.5281/zenodo.14386285	FIGURE 6. Typical results of ITS2 sequencing of Simulium reptans s.l. A–with nucleotide A in the position in question, B –with A/T (W) superposition, C–with nucleotide T.	FIGURE 6. Typical results of ITS2 sequencing of Simulium reptans s.l. A–with nucleotide A in the position in question, B –with A/T (W) superposition, C–with nucleotide T.	2024-12-04	Vaulin, Oleg V.;Petrozhitskaya, Liudmila V.;Novgorodova, Tatiana A.		Zenodo	biologists	Vaulin, Oleg V.;Petrozhitskaya, Liudmila V.;Novgorodova, Tatiana A.			
03CD521DFFED8178FF1B4B45FEAF83B3.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14386289/files/figure.png	https://doi.org/10.5281/zenodo.14386289	FIGURE 7. Maximum likelihood tree constructed from the DNA fragment of the COI gene of S. reptans. A branch of S. reptantoides was used for rooting. The geographical origin of the individuals is indicated. Sequences taken from the NCBI database are designated by corresponding numbers. Bayesian posterior probabilities (above the branches) and bootstrap values (below the branches) are given.	FIGURE 7. Maximum likelihood tree constructed from the DNA fragment of the COI gene of S. reptans. A branch of S. reptantoides was used for rooting. The geographical origin of the individuals is indicated. Sequences taken from the NCBI database are designated by corresponding numbers. Bayesian posterior probabilities (above the branches) and bootstrap values (below the branches) are given.	2024-12-04	Vaulin, Oleg V.;Petrozhitskaya, Liudmila V.;Novgorodova, Tatiana A.		Zenodo	biologists	Vaulin, Oleg V.;Petrozhitskaya, Liudmila V.;Novgorodova, Tatiana A.			
03CD521DFFED8178FF1B4B45FEAF83B3.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14386277/files/figure.png	https://doi.org/10.5281/zenodo.14386277	FIGURE 3. Proportions of two restriction spectra of Simulium reptans s.l. among the studied material collected in different localities in the territory of Ob-Irtysh basin. Si16, Si19, Si28–sample numbers; ns–non-significant differences (Fisher’s exact test, two-tailed, with Bonferroni correction, p> 0.017).	FIGURE 3. Proportions of two restriction spectra of Simulium reptans s.l. among the studied material collected in different localities in the territory of Ob-Irtysh basin. Si16, Si19, Si28–sample numbers; ns–non-significant differences (Fisher’s exact test, two-tailed, with Bonferroni correction, p> 0.017).	2024-12-04	Vaulin, Oleg V.;Petrozhitskaya, Liudmila V.;Novgorodova, Tatiana A.		Zenodo	biologists	Vaulin, Oleg V.;Petrozhitskaya, Liudmila V.;Novgorodova, Tatiana A.			
03CD521DFFEF8176FF1B4A50FBF08438.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14386281/files/figure.png	https://doi.org/10.5281/zenodo.14386281	FIGURE 4. Proportions of two restriction spectra among the studied material of Simulium reptans s.l. collected in different localities. North Europe (MR)–Murmansk Region (Russia); Western Siberia (NR+PR)—Novosibirsk Region (Russia) and Pavlodar Region (North Kazakhstan); Eastern Siberia (IR)–Irkutsk Region (Russia). The data marked with different letters are significantly different (Fisher’s exact test with Bonferroni correction, p <0.017).	FIGURE 4. Proportions of two restriction spectra among the studied material of Simulium reptans s.l. collected in different localities. North Europe (MR)–Murmansk Region (Russia); Western Siberia (NR+PR)—Novosibirsk Region (Russia) and Pavlodar Region (North Kazakhstan); Eastern Siberia (IR)–Irkutsk Region (Russia). The data marked with different letters are significantly different (Fisher’s exact test with Bonferroni correction, p <0.017).	2024-12-04	Vaulin, Oleg V.;Petrozhitskaya, Liudmila V.;Novgorodova, Tatiana A.		Zenodo	biologists	Vaulin, Oleg V.;Petrozhitskaya, Liudmila V.;Novgorodova, Tatiana A.			
