taxonID	type	description	language	source
0386492CFFFB3F4D9AE5FF14295AFCF2.taxon	materials_examined	Holotype: — HKAS 148727 Etymology: — The species epithet refers to the host genus where the fungus was collected. Figure 2. Conioscypha pruni (HKAS 148727, holotype). a – i Colonies on the natural substrate. a – c Colonies. d conidiogenous cells with attached conidia. e-i Conidia. j Germinating conidium. k, l Colony on PDA from above and below. m – q Sporulation observed on PDA. m, n Conidia and conidiogenous cell. o – q Conidia. Scale bars: d – j, m – q = 10 μm. Saprobic on dead branches of sweet cherry trees. Sexual morph: Undetermined. Asexual morph: Hyphomycetous. Colonies on natural substratum, effuse, scattered to gregarious, brown, shiny, superficial. Mycelium partly superficial, composed of septate, branched, subhyaline hyphae. Conidiophores macronematous, often reduced to conidiogenous cells. Conidiogenous cells 2.5 – 4 μm wide (x ̄ = 2.91 μm, n = 10), enteroblastic, with a small cup-like collarette, hyaline, and smooth-walled. Conidia 6 – 10 × 3.5 – 5 μm (x ̄ = 7.52 × 4.53 μm, n = 30), pleuroacrogenous, aseptate, guttulate, cylindrical, with rounded apices, pale brown when young, dark brown when mature, smooth-walled. Culture characteristics: Conidium germinated on PDA within 24 h. Colonies reaching a diameter of about 20 mm after 3 months at 25 ° C, colonies from above: irregular margins, coriaceous, dry, pale yellow, distinct black punctiform pigmentation from the reverse. Mycelium hyaline, 1.5 – 2.5 µm wide. Conidiophores often reduced to conidiogenous cells, Conidiogenous cells hyaline, 2.0 – 2.5 µm wide, with cup-like collarette. Conidia hyaline to brown, subglobose to ellipsoidal, 6 – 13 × 3.5 – 6 μm (x ̄ = 8.39 × 4.36 μm, n = 30). Material examined: CHINA. Sichuan Province: Chengdu City, Yunhua Mountain, Dujiangyan County, 31 ° 00 ' 74 " N, 103 ° 56 ' 19 " E, 1535 m, on the decaying branches of sweet cherry, 22 March 2024, Yan Jin, YHS 5 _ 1 (HKAS 148727, holotype), ex-type living culture CGMCC 3.29000 = UESTCC 24.0251, HUEST 24.0263, isotype. Notes: Phylogenetic analysis based on the combined dataset of ITS, LSU, SSU, RPB 2, and TEF 1 loci revealed that our collection (HUEST 24.0263) forms a monophyletic clade sister to C. minutispora (FIGURE 1). In the NCBI BLASTn search, the closest match to our isolate (UESTCC 24.0251) is C. minutispora. BLASTn analysis revealed 5.2 % differences in ITS (436 / 460 bp, 8 gaps), 5.2 % differences in LSU (626 / 661 bp, without gaps), and 99 % sequence identity in SSU (938 / 940 bp, 2 gaps) between UESTCC 24.0251 and C. minutispora. Morphologically, UESTCC 24.0251 differs from C. minutispora by comparatively shorter conidiogenous cells (2.5 – 4 μm vs. 4 – 5 μm) and by narrower conidia (3.5 – 5 μm vs. 5 – 6 μm). Conidia of the new species are guttulate, ellipsoidal, contrasting with C. minutispora, which produces conidia with distinctly truncate bases and a central pore. Therefore, based on morphological and phylogenetic evidence, our isolate is proposed as a new species.	en	Liao, Yue-Chi, Deng, Li-Ping, Chen, Yan-Peng, Liu, Jian-Kui, Maharachchikumbura, Sajeewa S. N. (2025): Conioscypha pruni sp. nov. (Conioscyphaceae, Conioscyphales), from sweet cherry (Prunus avium) from Sichuan Province, China. Phytotaxa 716 (2): 117-127, DOI: 10.11646/phytotaxa.716.2.4, URL: https://doi.org/10.11646/phytotaxa.716.2.4
