taxonID	type	description	language	source
038287C0EF24B366D4F736B5FF2A652A.taxon	materials_examined	Host of present specimens: Bufo melanostictus (Schneider) (Anura: Bufonidae). Locality of present specimens: Shuiman, Wuzhishan Mountain, Hainan Province. Site in host: Intestine. Prevalence and intensity of infections: Ten out of 39 individuals of B. melanostictus infected with intensity of 1 – 8 (mean 2.7) acanthocephalans. Voucher specimens: Nine male, 10 females (HBNU – A – A 2023015 ZL); deposited in the College of Life Sciences, Hebei Normal University, Hebei Province, China. Representative DNA sequences: Representative genetic data were deposited in the National Center for Biotechnology Information (NCBI) database (http: // www. ncbi. nlm. nih. gov) under the accession numbers: PP 447459, PP 447460 (18 S); PP 466919, PP 466920 (ITS); PP 476963, PP 476964 (cox 1); PP 486177, PP 486178 (cox 2); PP 471562, PP 471563 (12 S); PP 476192 (mitogenome).	en	Zhao, Cui-Hong, Yang, Rui-Jia, Ru, Si-Si, Chen, Hui-Xia, Li, Dai-Xuan, Li, Liang (2024): Integrative taxonomy of the genus PSeUDoaCanthoCephaLUS (Acanthocephala: Echinorhynchida) in China, with the description of two new species and the characterization of the mitochondrial genomes of PSeUDoaCanthoCephaLUS SIChUanenSIS sp. n. and PSeUDoaCanthoCephaLUS nGUYenthILeae. Parasites & Vectors (541) 17 (1): 1-20, DOI: 10.1186/s13071-024-06528-7, URL: https://doi.org/10.1186/s13071-024-06528-7
038287C0EF24B366D4F736B5FF2A652A.taxon	discussion	Remarks In the present study, the morphology of P. nguyenthileae was observed using light microscopy and, for the first time, scanning electron microscopy based on newly collected specimens from Bufo melanostictus (Schnei- der) in China. Te morphology and morphometric data of the present specimens agreed well with the original description of P. nguyenthileae [1], including the size of the trunk, neck, lemnisci, proboscis receptacle and testis; the size and armature of the proboscis; the number and arrangement of cement glands (4 long and 4 clavate); and the size of eggs (See Table 1 for details). Furthermore, the present specimens and some of Amin et al. ’ s specimens were both collected from the same host B. melanostictus in a neighboring region of Southeast Asia [1]. Consequently, we considered that the present specimens belong to P. nguyenthileae. Te position of the genital pore of this species appears variable in both males and females from different hosts. Te present study also sequenced the partial cox 1, cox 2 and 12 S data of P. nguyenthileae for the first time, which displayed low level of intraspecific variation only in the partial cox 1 (1.52 %) regions between different individuals of P. nguyenthileae. Tese genetic data are very useful for molecular identification of this species in the future. Tis is the first report of this species in China.	en	Zhao, Cui-Hong, Yang, Rui-Jia, Ru, Si-Si, Chen, Hui-Xia, Li, Dai-Xuan, Li, Liang (2024): Integrative taxonomy of the genus PSeUDoaCanthoCephaLUS (Acanthocephala: Echinorhynchida) in China, with the description of two new species and the characterization of the mitochondrial genomes of PSeUDoaCanthoCephaLUS SIChUanenSIS sp. n. and PSeUDoaCanthoCephaLUS nGUYenthILeae. Parasites & Vectors (541) 17 (1): 1-20, DOI: 10.1186/s13071-024-06528-7, URL: https://doi.org/10.1186/s13071-024-06528-7
038287C0EF20B362D74D36A7FAA266E8.taxon	description	Te complete mitogenomes of P. sichuanensis and P. nguyenthileae have 15812 bp and 13701 bp, respectively, both of which include 36 genes, containing 12 PCGs (cox 1 – 3, cytb, nad 1 – 6, nad 4 L and atp 6, missing atp 8), 22 transfer RNA (tRNA) genes and two rRNA genes (rrnL and rrnS) (Fig. 11; Additional file 7: Table S 4; Additional file 8: Table S 5). Two non-coding regions (NCRs) are present in the mitogenomes of P. sichuanensis and P. nguyenthileae (NCR 1 is 2282 bp in P. sichuanensis vs only 202 bp in P. nguyenthileae, both between tRNA-Trp and tRNA-Val; NCR 2 is 650 bp in P. sichuanensis vs 618 bp in P. nguyenthileae, both between tRNA-Ile and tRNA-Met) (Fig. 11; Additional file 7: Table S 4; Additional file 8: Table S 5). All genes are transcribed from the same strand. Te overall A + T content in the mitogenomes of P. sichuanensis and P. nguyenthileae is 56.8 % and 56.3 %, respectively, with both displaying a strong nucleotide compositional bias toward A + T. Te nucleotide content of P. sichuanensis and P. nguyenthileae mitogenomes are provided in Additional file 9: Table S 6. Taken together, the 12 PCGs of the mitogenomes of P. sichuanensis and P. nguyenthileae are 10,179 bp and 10,206 bp in length (excluding termination codons), with each gene ranging in size from 240 bp (nad 4 L) to 1627 bp (nad 5), which encoded 3391 and 3401 amino acids, respectively (Additional file 7: Table S 4; Additional file 8: Table S 5). Among the 12 PCGs of P. sichuanensis and P. nguyenthileae, six genes (cox 1, cox 3, nad 4 L, nad 1, nad 2 and nad 5) used GTG as the start codon, whereas two genes (cox 2 and atp 6) in P. sichuanensis and four genes (cox 2, nad 3, nad 6 and atp 6) in P. nguyenthileae used ATG as the start codon. TTG was used by the nad 4 and cytb genes in both species. TTG was used by the nad 6 gene and ATA was used by the nad 3 as the start codon in P. sichuanensis. TAA was the most commonly used termination codon in both species (4 genes [cox 3, nad 2, nad 3, nad 4 L] in P. sichuanensis vs 3 genes [cox 3, nad 2, nad 3] in P. nguyenthileae); two genes (cox 1 and cytb) in P. sichuanensis and three genes (cox 1, nad 4 L and cytb) in P. nguyenthileae used TAG as termination codon. Te incomplete termination codon T was inferred for the cox 2, nad 1, nad 4, nad 5, nad 6 and atp 6 genes in both species. Data on the component and usages of codons in the mitogenomes of P. sichuanensis and P. nguyenthileae are provided in Additional file 1: Figure S 1; Additional file 7: Table S 4; Additional file 8: Table S 5. Tere are 22 tRNAs and two rRNAs (rrnL located between tRNA-Tyr and tRNA-Leu 1; rrnS located between tRNA-Met and tRNA-Phe) in the mitogenomes of P. sichuanensis and P. nguyenthileae (Fig. 11; Additional file 7: Table S 4; Additional file 8: Table S 5). Te length of the 22 tRNAs and their anticodon secondary structures of P. sichuanensis and P. nguyenthileae are provided in Additional file 2: Figure S 2; Additional file 3: Figure S 3; Additional file 7: Table S 4; Additional file 8: Table S 5. Te gene arrangement of the 36 genes in the mitogenomes of P. sichuanensis and P. nguyenthileae are identical, both in the following order: cox 1, tRNA-Gly, tRNA-Gln, tRNA- Tyr, rrnL, tRNA-Leu 1, nad 6, tRNA- Asp, atp 6, nad 3, tRNA-Trp, tRNA-Val, tRNA-Lys, tRNA- Glu, tRNA-Tr, tRNA-Ser 2, nad 4 L, nad 4, tRNA-His, nad 5, tRNA-Leu 2, tRNA-Pro, cyt b, nad 1, tRNA-Ile, tRNA-Met, rrnS, tRNA-Phe, cox 2, tRNA-Cys, cox 3, tRNA- Ala, tRNA-Arg, tRNA-Asn, tRNA-Ser 1, nad 2 (Fig. 11).	en	Zhao, Cui-Hong, Yang, Rui-Jia, Ru, Si-Si, Chen, Hui-Xia, Li, Dai-Xuan, Li, Liang (2024): Integrative taxonomy of the genus PSeUDoaCanthoCephaLUS (Acanthocephala: Echinorhynchida) in China, with the description of two new species and the characterization of the mitochondrial genomes of PSeUDoaCanthoCephaLUS SIChUanenSIS sp. n. and PSeUDoaCanthoCephaLUS nGUYenthILeae. Parasites & Vectors (541) 17 (1): 1-20, DOI: 10.1186/s13071-024-06528-7, URL: https://doi.org/10.1186/s13071-024-06528-7
