identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
038187E49866FFD877DEAAE1FA54F9E5.text	038187E49866FFD877DEAAE1FA54F9E5.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Dna extraction PCR	<div><p>DNA extraction, PCR amplification and sequencing</p><p>Genomic DNA was extracted from colonies grown on PDA, using a modified cetyltrimethylammonium bromide (CTAB) protocol a kit method (OGPLF-400, GeneOnBio Corporation, Changchun, China) take for sanger sequencing (Guo et al. 2000; Zhao et al. 2023). The internal transcribed spacer regions with intervening 5.8S nrRNA gene (ITS), the partial large subunit (LSU) nrRNA, part of the beta-tubulin gene region (tub), and partial RNA polymerase II second largest subunit (rpb2) genes were amplified and sequenced by using primers pairs ITS5/ITS4 (White et al. 1990), LR0R/LR5 (Rehner &amp; Samuels 1994, Vilgalys &amp; Hester 1990), Bt2a/Bt2b (Glass &amp; Donaldson 1995), fRPB2- 5F/fRPB2-7cR (Liu et al. 1999, Sung et al. 2007).</p><p>The PCR was performed using an Eppendorf Master Thermocycler (Hamburg, Germany). Amplification reactions were performed in a 25 μL reaction volume, which contained 12.5 μL Green Taq Mix ( Vazyme, Nanjing, China), 1 μL of each forward and reverse primer (10 μM) ( TsingKe, Qingdao, China), and 1 μL template genomic DNA in amplifier, adjusted with distilled deionized water to a total volume of 25 μL The PCR parameters were as follows: 94 °C for 5 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at a suitable temperature for 50 s, extension at 72 °C for 1 min and a final elongation step at 72°C for 10 min. Annealing temperature for each gene were 55 °C for ITS, 52 °C for LSU, 53 °C for tub and 56 °C for rpb2. The PCR products were separated with the 1 % agarose gel, and add GelRed and use UV light to visualize the fragments. Sequencing was done bi-directionally, conducted by the Biosune Company Limited (Shanghai, China). Consensus sequences were obtained using MEGA 7.0 (Kumar et al. 2016). New sequences generated in this study were deposited at NCBI’s GenBank (www.ncbi.nlm.nih.gov; Table 1) .</p></div>	https://treatment.plazi.org/id/038187E49866FFD877DEAAE1FA54F9E5	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	He, Yong-Ze	He, Yong-Ze (2025): Neopyrenochaetopsis machili sp. nov. on Machilus nanmu from Shanxi Province, China. Phytotaxa 696 (3): 224-234, DOI: 10.11646/phytotaxa.696.3.3, URL: https://doi.org/10.11646/phytotaxa.696.3.3
038187E49862FFDF77DEA9D1FCE5FDA3.text	038187E49862FFDF77DEA9D1FCE5FDA3.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Neopyrenochaetopsis machili Y. Z. He 2025	<div><p>Neopyrenochaetopsis machili Y.Z. He, sp. nov.</p><p>MycoBank: 856714</p><p>Fig. 2</p><p>Etymology. The specific epithet refers to the genus of the host plant Machilus nanmu .</p><p>Type. China, Shanxi Province: Taiyuan City, <a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=112.5&amp;materialsCitation.latitude=37.8" title="Search Plazi for locations around (long 112.5/lat 37.8)">Longcheng Forest</a> Park, on diseased leaves of Machilus nanmu (Oliv.) Hemsl. (1200m; 37.8°N, 112.5°E), 15 Oct. 2023, Y. Z. He, holotype HSAUP1175, ex-type CGMCC3.28266 .</p><p>Description. Colonies on PDA incubated at 25 °C in the dark, reaching 73–76 mm diam., margin scalloped or irregularly round, center olive green and flavogreen edges, aerial mycelium moderately developed, reverse similarity. Conidiomata solitary or aggregated, formed on agar surface, black, globose to subglobose, 150–250 × 150–300 mm. Conidiophores are inapparent and often reduced to conidiogenous cells. Conidiogenous cells phialidic, ampulliform, straight or slightly curved, slightly branched, 4.2–7.7 × 2–2.6. Conidia solitary, obovoid to ellipsoid, 2.8–3.8 × 1.5–2.2 μm, mean ± SD = 3.3 ± 0.2 × 1.8 ± 0.2 μm, hyaline, 1–2 guttulate, smooth, apex obtuse, base with inconspicuous hilum. Sexual morph not observed.</p><p>Additional specimens examined: China, Shanxi Province: Taiyuan City, on leaves of Machilus nanmu (Oliv.) Hemsl., 15 Oct. 2023, Y.Z. He, HSAUP1125, living culture H1125.</p><p>Notes. Phylogenetic analyses showed that Neopyrenochaetopsis machili formed an independent clade closely related to Neopyrenochaetopsis hominis CBS 143033 (Fig. 1). Neopyrenochaetopsis machili was distinguished from N. hominis (CBS 143033) by 5/815 base pairs in the LSU, 47/ 537 in the ITS, 50/ 304 in the tub, and 153/ 874 in the rpb2, including gaps. The conidia of N. machili were similar to those of N. hominis (2.8–3.8 × 1.5–2.2 vs. 3–3.5 × 1.5–2 µm), but the conidiomata and colonies of N. machili differed from those of N. hominis (black vs. brown). The new isolates were described here as a new species based on their distinct morphological characteristics and DNA based differences as recommended by Jeewon and Hyde (2016).</p></div>	https://treatment.plazi.org/id/038187E49862FFDF77DEA9D1FCE5FDA3	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	He, Yong-Ze	He, Yong-Ze (2025): Neopyrenochaetopsis machili sp. nov. on Machilus nanmu from Shanxi Province, China. Phytotaxa 696 (3): 224-234, DOI: 10.11646/phytotaxa.696.3.3, URL: https://doi.org/10.11646/phytotaxa.696.3.3
