taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
03ADC404FF9C327C4B92FF11DEAD9381.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/17650818/files/figure.png	https://doi.org/10.5281/zenodo.17650818	Figure 2. Pelagotrichidium faurei from life (A, B, F, G, bright field; C–E, differential interference contrast) and afer protargol impregnation (H–M). A, ventral view of a typical individual.B, lef lateral view.C, ventral view of a squeezed living cell; arrow marks the contractile vacuole. D, ventral view to show the colourless cortical granules. E, macronuclear nodules and micronuclei. F, ventral view of the posterior right margin of the body, showing the food vacuoles and algae. G, ventral view showing a different body shape. H, I, ventral and dorsal views of a specimen to show the infraciliature and nuclear apparatus.Te engulfed algae are likely to be Cryptophyta.(Please note: its posterior end bent, resulting in a curved posterior end of the lef marginal row; in 19 protargol-stained specimens, approximately half the cells exhibited body torsion, causing the paroral and endoral membranes to intersect in the middle, as shown in H). J, K, ventral views of frontoventral cirral row I in different cells. L, dorsal view of posterior region, showing typical posterior end of lef marginal row. M, ventral view, showing the migrating frontoventral cirral row I and dorsal kinety 1 in the opisthe afer division. Abbreviations: DK1, dorsal kinety 1; LMR, lef marginal cirral row; Ma, macronuclear nodules; Mi, micronuclei.Scale bars: 70 μm.	Figure 2. Pelagotrichidium faurei from life (A, B, F, G, bright field; C–E, differential interference contrast) and afer protargol impregnation (H–M). A, ventral view of a typical individual.B, lef lateral view.C, ventral view of a squeezed living cell; arrow marks the contractile vacuole. D, ventral view to show the colourless cortical granules. E, macronuclear nodules and micronuclei. F, ventral view of the posterior right margin of the body, showing the food vacuoles and algae. G, ventral view showing a different body shape. H, I, ventral and dorsal views of a specimen to show the infraciliature and nuclear apparatus.Te engulfed algae are likely to be Cryptophyta.(Please note: its posterior end bent, resulting in a curved posterior end of the lef marginal row; in 19 protargol-stained specimens, approximately half the cells exhibited body torsion, causing the paroral and endoral membranes to intersect in the middle, as shown in H). J, K, ventral views of frontoventral cirral row I in different cells. L, dorsal view of posterior region, showing typical posterior end of lef marginal row. M, ventral view, showing the migrating frontoventral cirral row I and dorsal kinety 1 in the opisthe afer division. Abbreviations: DK1, dorsal kinety 1; LMR, lef marginal cirral row; Ma, macronuclear nodules; Mi, micronuclei.Scale bars: 70 μm.	2025-07-01	Hong, Meijie;Zhang, Zihui;Pan, Hongbo;Jiang, Jiamei		Zenodo	biologists	Hong, Meijie;Zhang, Zihui;Pan, Hongbo;Jiang, Jiamei			
03ADC404FF9C327C4B92FF11DEAD9381.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/17650822/files/figure.png	https://doi.org/10.5281/zenodo.17650822	Figure 3. Pelagotrichidium faurei from life (A, B, E) and afer protargol impregnation (C, D, F–H). A, ventral view of a typical individual; arrow indicates the contractile vacuole.B, lef lateral view, showing the food vacuoles and the algae in the cytoplasm. C, D, ventral (C) and dorsal (D) views of a specimen, to show the infraciliature and nuclear apparatus.E, cortical granules. F, ventral view of the anterior region, showing the undulating membranes, frontoventral cirral rows, and right marginal cirral row.G, H, details of basal bodies in frontoventral cirral rows I–III of the specimen in C (G) and another individual (H). Please note that the lefmost cirrus of frontoventral I is reduced into a basal pair in some individuals (F and G). Abbreviations:AZM, adoral zone of membranelles; EM, endoral membrane; LMR, lef marginal cirral row; Ma, macronuclear nodules; Mi, micronuclei; PM, paroral membrane; RMR, right marginal cirral row; I–V, frontoventral cirral rows I–V; 1–5, dorsal kineties 1–5. Scale bars: 70 μm.	Figure 3. Pelagotrichidium faurei from life (A, B, E) and afer protargol impregnation (C, D, F–H). A, ventral view of a typical individual; arrow indicates the contractile vacuole.B, lef lateral view, showing the food vacuoles and the algae in the cytoplasm. C, D, ventral (C) and dorsal (D) views of a specimen, to show the infraciliature and nuclear apparatus.E, cortical granules. F, ventral view of the anterior region, showing the undulating membranes, frontoventral cirral rows, and right marginal cirral row.G, H, details of basal bodies in frontoventral cirral rows I–III of the specimen in C (G) and another individual (H). Please note that the lefmost cirrus of frontoventral I is reduced into a basal pair in some individuals (F and G). Abbreviations:AZM, adoral zone of membranelles; EM, endoral membrane; LMR, lef marginal cirral row; Ma, macronuclear nodules; Mi, micronuclei; PM, paroral membrane; RMR, right marginal cirral row; I–V, frontoventral cirral rows I–V; 1–5, dorsal kineties 1–5. Scale bars: 70 μm.	2025-07-01	Hong, Meijie;Zhang, Zihui;Pan, Hongbo;Jiang, Jiamei		Zenodo	biologists	Hong, Meijie;Zhang, Zihui;Pan, Hongbo;Jiang, Jiamei			
03ADC404FF9C327C4B92FF11DEAD9381.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/17650826/files/figure.png	https://doi.org/10.5281/zenodo.17650826	Figure 4. Drawings showing very early to middle morphogenetic stages of Pelagotrichidium faurei afer protargol staining.A, B, ventral views of a very early divider, showing the newly formed oral primordium of the opisthe near frontoventral cirral row IV (for details, see B); arrows in A show the replication bands, and arrow in B shows the intact old cirrus.C, ventral view of the oral primordium in a later stage. D–F, ventral views (D, E) and dorsal view (F) of an early divider, showing the occurrence of the frontoventral cirral anlagen (I–V); E shows the details of the oral primordium of the opisthe, and arrowheads in F show dorsal kinety anlagen (1–3). G, ventral view of an early middle divider, showing the assembly of new adoral membranelles; double arrowhead shows the dedifferentiation of the parental undulating membranes.H, ventral view of a middle divider having six frontoventral anlagen in the proter. I, J, ventral (I) and dorsal (J) views of a middle divider bearing five frontoventral anlagen, a fused macronucleus, and a fused micronucleus. Abbreviations: FVR IV, frontoventral cirral row IV; LMA, lef marginal cirral row anlage; Ma, macronuclear nodules; Mi, micronuclei; OP, oral primordium; RMA, right marginal cirral row anlage; I–VI, frontoventral cirral anlagen I–VI; 1–5, dorsal kineties 1–5. Scale bars: 70 μm.	Figure 4. Drawings showing very early to middle morphogenetic stages of Pelagotrichidium faurei afer protargol staining.A, B, ventral views of a very early divider, showing the newly formed oral primordium of the opisthe near frontoventral cirral row IV (for details, see B); arrows in A show the replication bands, and arrow in B shows the intact old cirrus.C, ventral view of the oral primordium in a later stage. D–F, ventral views (D, E) and dorsal view (F) of an early divider, showing the occurrence of the frontoventral cirral anlagen (I–V); E shows the details of the oral primordium of the opisthe, and arrowheads in F show dorsal kinety anlagen (1–3). G, ventral view of an early middle divider, showing the assembly of new adoral membranelles; double arrowhead shows the dedifferentiation of the parental undulating membranes.H, ventral view of a middle divider having six frontoventral anlagen in the proter. I, J, ventral (I) and dorsal (J) views of a middle divider bearing five frontoventral anlagen, a fused macronucleus, and a fused micronucleus. Abbreviations: FVR IV, frontoventral cirral row IV; LMA, lef marginal cirral row anlage; Ma, macronuclear nodules; Mi, micronuclei; OP, oral primordium; RMA, right marginal cirral row anlage; I–VI, frontoventral cirral anlagen I–VI; 1–5, dorsal kineties 1–5. Scale bars: 70 μm.	2025-07-01	Hong, Meijie;Zhang, Zihui;Pan, Hongbo;Jiang, Jiamei		Zenodo	biologists	Hong, Meijie;Zhang, Zihui;Pan, Hongbo;Jiang, Jiamei			
03ADC404FF9C327C4B92FF11DEAD9381.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/17650828/files/figure.png	https://doi.org/10.5281/zenodo.17650828	Figure 5. Drawings showing late to very late morphogenetic stages of Pelagotrichidium faurei afer protargol staining.A, B, ventral (A) and dorsal (B) views of a late divider; arrows show the anlage of dorsal kinety 4, and arrowheads show the anlage of dorsal kinety 5. C, D, ventral (C) and dorsal (D) views of a very late divider bearing six frontoventral anlagen in both the proter and the opisthe. Abbreviations: LMA, lef marginal cirral row anlage; LMR, lef marginal cirral row; Ma, macronuclear nodules; Mi, micronuclei; RMR, right marginal cirral row; I–VI, frontoventral cirral anlagen I–VI; 1–5, dorsal kineties 1–5. Scale bars: 70 μm.	Figure 5. Drawings showing late to very late morphogenetic stages of Pelagotrichidium faurei afer protargol staining.A, B, ventral (A) and dorsal (B) views of a late divider; arrows show the anlage of dorsal kinety 4, and arrowheads show the anlage of dorsal kinety 5. C, D, ventral (C) and dorsal (D) views of a very late divider bearing six frontoventral anlagen in both the proter and the opisthe. Abbreviations: LMA, lef marginal cirral row anlage; LMR, lef marginal cirral row; Ma, macronuclear nodules; Mi, micronuclei; RMR, right marginal cirral row; I–VI, frontoventral cirral anlagen I–VI; 1–5, dorsal kineties 1–5. Scale bars: 70 μm.	2025-07-01	Hong, Meijie;Zhang, Zihui;Pan, Hongbo;Jiang, Jiamei		Zenodo	biologists	Hong, Meijie;Zhang, Zihui;Pan, Hongbo;Jiang, Jiamei			
03ADC404FF9C327C4B92FF11DEAD9381.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/17650830/files/figure.png	https://doi.org/10.5281/zenodo.17650830	Figure 6. Photomicrographs of Pelagotrichidium faurei afer protargol preparation during morphogenetic process. A, ventral view of a very early divider to show oral primordium in the opisthe; arrows show replication bands in each macronuclear nodule. B, ventral view to show an enlarged oral primordium along frontoventral cirrial row IV in a later stage. C, D, ventral and dorsal views of an early divider, showing frontoventral cirral anlagen I–V in the opisthe (C); arrowhead in D shows initiation of dorsal kineties anlage 1. E, F, ventral (E) and dorsal (F) views of an early middle divider to show frontoventral cirral anlagen I–V, lef marginal cirral anlage, and dorsal kineties anlagen 2 and 3. G, H, ventral views of the proter (G) and the opisthe (H) of a middle divider; the proter has six frontoventral cirral anlagen. I, J, dorsal views in later stages to show development of dorsal kineties anlagen (I) and fused nuclei (J). K, L, ventral views of a late divider to show newly formed frontoventral cirral rows, spliting macronucleus and splited micronuclei (K), and details of new formed dorsomarginal kineties in the opisthe (L). M, ventral view of a very late divider to show newly formed dorsal kineties 4 and 5 (circled). Abbreviations: LMA, lef marginal cirral row anlage; Ma, macronuclear nodules; Mi, micronuclei; OP, oral primordium; RMA, right marginal cirral row anlage; I–VI, frontoventral cirral anlagen I–VI; 2–5, dorsal kinety anlagen.Scale bars: 70 μm.	Figure 6. Photomicrographs of Pelagotrichidium faurei afer protargol preparation during morphogenetic process. A, ventral view of a very early divider to show oral primordium in the opisthe; arrows show replication bands in each macronuclear nodule. B, ventral view to show an enlarged oral primordium along frontoventral cirrial row IV in a later stage. C, D, ventral and dorsal views of an early divider, showing frontoventral cirral anlagen I–V in the opisthe (C); arrowhead in D shows initiation of dorsal kineties anlage 1. E, F, ventral (E) and dorsal (F) views of an early middle divider to show frontoventral cirral anlagen I–V, lef marginal cirral anlage, and dorsal kineties anlagen 2 and 3. G, H, ventral views of the proter (G) and the opisthe (H) of a middle divider; the proter has six frontoventral cirral anlagen. I, J, dorsal views in later stages to show development of dorsal kineties anlagen (I) and fused nuclei (J). K, L, ventral views of a late divider to show newly formed frontoventral cirral rows, spliting macronucleus and splited micronuclei (K), and details of new formed dorsomarginal kineties in the opisthe (L). M, ventral view of a very late divider to show newly formed dorsal kineties 4 and 5 (circled). Abbreviations: LMA, lef marginal cirral row anlage; Ma, macronuclear nodules; Mi, micronuclei; OP, oral primordium; RMA, right marginal cirral row anlage; I–VI, frontoventral cirral anlagen I–VI; 2–5, dorsal kinety anlagen.Scale bars: 70 μm.	2025-07-01	Hong, Meijie;Zhang, Zihui;Pan, Hongbo;Jiang, Jiamei		Zenodo	biologists	Hong, Meijie;Zhang, Zihui;Pan, Hongbo;Jiang, Jiamei			
03ADC404FF9932714BE9F900DC7292D3.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/17650833/files/figure.png	https://doi.org/10.5281/zenodo.17650833	Figure 7. Psilotrichides hawaiiensis from life (A, bright field; B–G, differential interference contrast) and afer protargol staining (H–L). A, ventral view of a representative individual. B, showing the ectosymbiotic bacteria on the cell surface (arrows). C, details of the green algae; arrowheads indicate the red eyespots. D, ventral view, to show the adoral zone.E, dorsal view, to show the contractile vacuole at slightly lef of midline; arrows show the dorsal bristles. F, dorsal view, to show the contractile vacuole at the lef body margin.G, the macronuclear nodules and micronucleus. H, I, ventral (H) and dorsal (I) views of a typical individual.J, ventral view of an individual bearing more ventral cirri. K, L, ventral (K) and dorsal (L) views to show the infraciliature and nuclear apparatus; arrows indicate the postoral cirral row. Abbreviations: AZM, adoral zone of membranelles; CV, contractile vacuole; EM, endoral membrane; LMR, lef marginal row; Ma, macronuclear nodules; Mi, Micronuclei; PM, paroral membrane; RMR, right marginal row; R1–4, ventral cirral row 1–4; 1–3, dorsal kinety 1–3. Scale bars: 20 μm.	Figure 7. Psilotrichides hawaiiensis from life (A, bright field; B–G, differential interference contrast) and afer protargol staining (H–L). A, ventral view of a representative individual. B, showing the ectosymbiotic bacteria on the cell surface (arrows). C, details of the green algae; arrowheads indicate the red eyespots. D, ventral view, to show the adoral zone.E, dorsal view, to show the contractile vacuole at slightly lef of midline; arrows show the dorsal bristles. F, dorsal view, to show the contractile vacuole at the lef body margin.G, the macronuclear nodules and micronucleus. H, I, ventral (H) and dorsal (I) views of a typical individual.J, ventral view of an individual bearing more ventral cirri. K, L, ventral (K) and dorsal (L) views to show the infraciliature and nuclear apparatus; arrows indicate the postoral cirral row. Abbreviations: AZM, adoral zone of membranelles; CV, contractile vacuole; EM, endoral membrane; LMR, lef marginal row; Ma, macronuclear nodules; Mi, Micronuclei; PM, paroral membrane; RMR, right marginal row; R1–4, ventral cirral row 1–4; 1–3, dorsal kinety 1–3. Scale bars: 20 μm.	2025-07-01	Hong, Meijie;Zhang, Zihui;Pan, Hongbo;Jiang, Jiamei		Zenodo	biologists	Hong, Meijie;Zhang, Zihui;Pan, Hongbo;Jiang, Jiamei			
03ADC404FF9932714BE9F900DC7292D3.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/17650816/files/figure.png	https://doi.org/10.5281/zenodo.17650816	Figure 1. Te sampling locations and habitats (A–D) and the phylogenetic tree based on SSU ribosomal DNA sequences (E). A, B, maps showing the sampling sites. C, D, photographs of the habitats of sampling sites, which are marked in B, where Pelagotrichidium faurei and Psilotrichides hawaiiensis, respectively, were collected. E, the maximum likelihood (ML) tree inferred from SSU ribosomal DNA sequences, showing the systematic position of Chinese populations of Pe.faurei and Ps. hawaiiensis (in red). Numbers at nodes represent the bootstrap values of ML analysis and the posterior probability of Bayesian analysis. Fully supported (1.00/100) branches are marked with filled circles. Asterisks (*) indicate the disagreement between Bayesian inference tree and the reference ML tree. All branches are drawn to scale. Scale bar corresponds to three substitutions per 100 nucleotide positions.	Figure 1. Te sampling locations and habitats (A–D) and the phylogenetic tree based on SSU ribosomal DNA sequences (E). A, B, maps showing the sampling sites. C, D, photographs of the habitats of sampling sites, which are marked in B, where Pelagotrichidium faurei and Psilotrichides hawaiiensis, respectively, were collected. E, the maximum likelihood (ML) tree inferred from SSU ribosomal DNA sequences, showing the systematic position of Chinese populations of Pe.faurei and Ps. hawaiiensis (in red). Numbers at nodes represent the bootstrap values of ML analysis and the posterior probability of Bayesian analysis. Fully supported (1.00/100) branches are marked with filled circles. Asterisks (*) indicate the disagreement between Bayesian inference tree and the reference ML tree. All branches are drawn to scale. Scale bar corresponds to three substitutions per 100 nucleotide positions.	2025-07-01	Hong, Meijie;Zhang, Zihui;Pan, Hongbo;Jiang, Jiamei		Zenodo	biologists	Hong, Meijie;Zhang, Zihui;Pan, Hongbo;Jiang, Jiamei			
