identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
03AB879DFFDF6A24FCDAFE9DA771FBB3.text	03AB879DFFDF6A24FCDAFE9DA771FBB3.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Anopheles larvae subsp. sampling	<div><p>Anopheles larvae sampling</p> <p>The larvae were collected with the aid of standard ladle with 350 mL volumetric capacity, an 11 cm opening and 1 m handle, in order to reach the areas around the larval habitats for 30 min at each site. The sampled individuals were separated in containers by habitat type and collection date, and fed three times a week with macerated fish feed (Tetra Marine Saltware, Tetra ® mixed 1:1 with Gold Fish Color, Alcon ®), with the aim of obtaining adults for identification (Scarpassa and Tadei, 1990; Oliveira et al., 2012).</p> <p>Due to the great difficulty in rearing Anopheles larvae in the laboratory, a simple and effective methodology was developed for development to the adult phase without significant losses: larvae were maintained in simulated environments, in trays with water from their respective breeding grounds (containing algae and organic material the larvae feed on) and floating macrophytes belonging to the Salvinia genus. This methodology was effective in the rearing and maintenance of the larvae in the laboratory.</p> <p>The sampled specimens were sent to the Malaria and Dengue Laboratory – LMD/COSAS/INPA, where they were kept under controlled temperature conditions, at 26 ± 2 ◦ C, upper relative humidity of 70–80% and a 12:12 h photoperiod. The larvae were maintained until adult emergence for identification using dichotomous keys (Gorham et al., 1967; Faran, 1980; Faran and Linthicum, 1981; Consoli and Lourenço-de-Oliveira, 1994).</p></div> 	https://treatment.plazi.org/id/03AB879DFFDF6A24FCDAFE9DA771FBB3	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Arcos, Adriano Nobre;Ferreira, Francisco Augusto da Silva;Cunha, Hillândia Brandão da;Tadei, Wanderli Pedro	Arcos, Adriano Nobre, Ferreira, Francisco Augusto da Silva, Cunha, Hillândia Brandão da, Tadei, Wanderli Pedro (2018): Characterization of artificial larval habitats of Anopheles darlingi (Diptera: Culicidae) in the Brazilian Central Amazon. Revista Brasileira de Entomologia 62 (4): 267-274, DOI: 10.1016/j.rbe.2018.07.006, URL: http://dx.doi.org/10.1016/j.rbe.2018.07.006
03AB879DFFDC6A27FF8CF8EDA655FA9A.text	03AB879DFFDC6A27FF8CF8EDA655FA9A.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Anopheles larvae	<div><p>Anopheles larvae</p> <p>All three types of larval habitats (fish ponds, clay pits and dams) were positive for the presence of anophelines, especially the vector of malaria in the Amazon.During the study, rainfall in the study area was high, averaging 203.8 mm, with a rainfall index at the larval habitats ranging from 51.8 to 253.4 mm per month.</p> <p>A total of 2.863 specimens were collected, comprising ten species. The most abundant species were An. triannulatus 1.286 (44.9%), An.darlingi 566 (19.7%) and An.nuneztovari 464 (16.2%).The dam larval habitats showed higher species abundance and richness. The larvae rate per man-hour was higher at dam’s larval habitats one, two and eight, with 7.0, 8.2 and 7.1 larvae collected per minute respectively (Table 1).</p> </div>	https://treatment.plazi.org/id/03AB879DFFDC6A27FF8CF8EDA655FA9A	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Arcos, Adriano Nobre;Ferreira, Francisco Augusto da Silva;Cunha, Hillândia Brandão da;Tadei, Wanderli Pedro	Arcos, Adriano Nobre, Ferreira, Francisco Augusto da Silva, Cunha, Hillândia Brandão da, Tadei, Wanderli Pedro (2018): Characterization of artificial larval habitats of Anopheles darlingi (Diptera: Culicidae) in the Brazilian Central Amazon. Revista Brasileira de Entomologia 62 (4): 267-274, DOI: 10.1016/j.rbe.2018.07.006, URL: http://dx.doi.org/10.1016/j.rbe.2018.07.006
