taxonID	type	description	language	source
BA5287B6FFD8FFA8FF2FFC0B8E718254.taxon	materials_examined	Type host: White-spotted thigh tree-frog Polypedates megacephalus (Hallowell) (Anura: Rhacophoridae). Type-locality: XiShuangBanNa Tropical Botanical Garden (21 ° 41 ′ N, 101 ° 25 ′ E), Yunnan Province, China. Type specimens: Holotype: male (HBNU – N- 2020 A 009 L); allotype: female (HBNU – N- 2020 A 010 L); paratypes: 41 males, 122 females (HBNU – N- 2020 A 011 L).	en	Chen, Hui-Xia, Gu, Xiao-Hong, Ni, Xue-Feng, Li, Liang (2021): Description of a new species of AplECTana (Nematoda: Ascaridomorpha: Cosmocercidae) using an integrative approach and preliminary phylogenetic study of Cosmocercidae and related taxa. Parasites & Vectors (165) 14 (1): 1-10, DOI: 10.1186/s13071-021-04667-9, URL: http://dx.doi.org/10.1186/s13071-021-04667-9
BA5287B6FFD8FFA8FF2FFC0B8E718254.taxon	description	Site of infection: Intestine. Prevalence and intensity of infection: 12.1 % (11 P. megacephalus infected out of 91 examined) were infected with intensity of 1 – 88 (mean 15.0) nematodes. ZooBank registration: To comply with the regulations set out in Article 8.5 of the amended 2012 version of the International Code of Zoological Nomenclature (ICZN) [19], details of the new species have been submitted to ZooBank. Te Life Science Identifier (LSID) of the article is urn: lsid: zoobank. org: pub: 09 F 4 B 1 EF-C 3 AF 42 E 6 - 80 E 6 - B 734 D 6 B 084 B 8. Te LSID for the new name Aplectana xishuangbannaensis is urn: lsid: zoobank. org: act: 5 E 4 C 6 C 18 - 7 B 72 - 4 C 28 - BD 28 - 6964 C 6 D 8 F 0 A 3.	en	Chen, Hui-Xia, Gu, Xiao-Hong, Ni, Xue-Feng, Li, Liang (2021): Description of a new species of AplECTana (Nematoda: Ascaridomorpha: Cosmocercidae) using an integrative approach and preliminary phylogenetic study of Cosmocercidae and related taxa. Parasites & Vectors (165) 14 (1): 1-10, DOI: 10.1186/s13071-021-04667-9, URL: http://dx.doi.org/10.1186/s13071-021-04667-9
BA5287B6FFD8FFA8FF2FFC0B8E718254.taxon	etymology	Etymology: Te specific epithet refers to the type location XiShuangBanNa Tropical Botanical Garden, Yunnan Province, China.	en	Chen, Hui-Xia, Gu, Xiao-Hong, Ni, Xue-Feng, Li, Liang (2021): Description of a new species of AplECTana (Nematoda: Ascaridomorpha: Cosmocercidae) using an integrative approach and preliminary phylogenetic study of Cosmocercidae and related taxa. Parasites & Vectors (165) 14 (1): 1-10, DOI: 10.1186/s13071-021-04667-9, URL: http://dx.doi.org/10.1186/s13071-021-04667-9
BA5287B6FFD8FFA8FF2FFC0B8E718254.taxon	description	Description General Small-sized, whitish nematodes. Body cylindrical, maximum width at about region of middle body. Cuticle with fine transverse striations and longitudinal stockade-like ornamentation (Fig. 1 a – c). Somatic papillae small, distributed irregularly over body surface (Figs. 1 a – c, e, i, 2 b). Lateral alae extending from 60 – 70 posterior to base of lips as far as about middle of tail in both sexes (Fig. 1 b, f, i). Oral aperture simple, triangular, surrounded by 3 small lips, each with inner flanges (Figs. 1 a, b, d, 2 b). Dorsal lip with pair of large double cephalic papillae; subventral lips with single large double cephalic papilla and amphid each (Figs. 1 a, 2 b). Oesophagus divided into anterior short pharynx, cylindrical corpus, slightly narrower isthmus and terminal posterior bulb with valves (Fig. 2 a). Nerve ring located at about 1 / 2 of oesophageal length. Excretory pore slightly anterior to of oesophageal bulb (Fig. 2 a). Tail of both sexes conical, with long filamentous tip (Figs. 1 e – g, i, 2 c, f, h). Male [Based on 10 mature specimens; Figs. 1 b, d – h, 2 a, f – h]: Body 2.32 – 2.72 (2.49) mm long, maximum width 139 – 178 (158). Oesophagus 317 – 426 (374) long, representing 12.6 – 16.1 (15.0) % of body length; pharynx + corpus + isthmus 248 – 356 (307) long, bulb 59 – 69 (67) × 50 – 59 (54) (Fig. 2 a). Nerve ring 158 – 198 (176) and excretory pore 257 – 376 (334) from anterior extremity, respectively (Fig. 2 a). Posterior end of body distinctly curved ventrally (Figs. 1 e, 2 f). Spicules small, similar in shape and length, 139 – 178 (161) long, distal end pointed, representing 5.98 – 7.09 (6.47) % of body length (Fig. 2 g). Gubernaculum absent. Caudal papillae: 6 pairs of precloacal, 3 pairs paracloacal (distinguishable from somatic papillae) and 4 pairs postcloacal papillae. Single median, ventral precloacal papilla present (Figs. 1 g, h, 2 h). Tail 198 – 248 (230) long, representing 8.26 ‒ 9.84 (9.26) % of body length (Figs. 1 e – g, 2 f, h). Female [Based on 10 mature specimens; Figs. 1 a, c, i, 2 b – e]: Body 3.54 – 3.86 (3.65) mm long, maximum width 248 – 297 (272). Oesophagus 416 – 446 (431) long, representing 11.0 – 12.6 (11.8) % of body length; pharynx + corpus + isthmus 347 – 366 (356) long, bulb 69 – 79 (74) × 50 – 69 (62). Nerve ring 208 – 228 (215) and excretory pore 347 – 386 (366) from anterior extremity, respectively. Vulva transverse slit, 1.60 – 2.10 (1.89) mm from anterior extremity, at 44.8 – 54.5 (51.8) % of body length. Ovaries two, located anterior to vulva. Vagina muscular (Fig. 2 d). Uteri amphidelphic, full of eggs in different stages of development; egg oval, large, with smooth surface, 149 – 297 (205) × 99 – 238 (146) (n = 20) (Fig. 2 e). Tail 347 – 406 (384) long, representing 9.78 ‒ 11.1 (10.5) % of body length (Figs. 1 i, 2 c). Genetic characterization Partial 18 S region Tree 18 S sequences of Aplectana xishuangbannaensis n. sp. (accession numbers MW 329041 – MW 329043) obtained were all 1539 bp long, representing only one genotype. Tere is no species of Aplectana with 18 S sequenced registered in GenBank. Pairwise comparison between A. xishuangbannaensis n. sp. and the other species of Cosmocercidae regarding the 18 S sequences available in GenBank, including Cosmocerca simile (MN 839758 – MN 839760), Cosmocercoides dukae (FJ 516753), C. pulcher (LC 018444, MH 178322 – MH 178326), C. qingtianensis (MH 032769 – MH 032771, MH 178319 – MH 178321), C. tonkinensis (AB 908160), C. wuyiensis (MK 110872), Nemhelix bakeri (DQ 118537) and Raillietnema sp. (DQ 503461), displayed 1.88 – 3.77 % nucleotide divergence. Partial ITS- 1 region Tree ITS- 1 sequences of A. xishuangbannaensis n. sp. (accession numbers MW 329035 – MW 329037) obtained were all 554 bp long, representing only one genotype. Tere are two species of Aplectana with ITS sequences available in GenBank, including A. chamaeleonis (MN 907375 ‒ MN 907378) and Aplectana sp. ’ Neyraplectana ’ PNLS- 530 (MH 836325). Pairwise comparison between A. xishuangbannaensis n. sp. and the previously mentioned taxa showed 46.67 and 45.47 % nucleotide divergence, respectively. Pairwise comparison between A. xishuangbannaensis n. sp. and the other species of Cosmocercidae regarding the ITS sequences available in GenBank, including Cosmocerca japonica (LC 052772 ‒ LC 052782), C. longicauda (MG 594349 ‒ MG 594351), C. ornata (MT 108302), Cosmocerca sp. LL- 2020 (MT 108303), C. simile (MN 839761 ‒ MN 839768), Cosmocercoides pulcher (MH 178314 – MH 178318, LC 018444), C. qingtianensis (MH 178311 – MH 178313, MH 032772 – MH 032774), C. tonkinensis (AB 908160, AB 908161) and C. wuyiensis (MK 110871), displayed 28.53 – 47.52 % of nucleotide divergence. Partial 28 S region Tree 28 S sequences of A. xishuangbannaensis n. sp. (accession numbers MW 329038 – MW 329040) obtained were all 740 bp long, representing only one genotype. Tere is only one species of Aplectana, Aplectana sp. ’ Neyraplectana ’ PNLS- 530, with 28 S sequence data (MH 909070) available in GenBank. Pairwise comparison between A. xishuangbannaensis n. sp. and the previously mentioned taxon showed 20.67 % of nucleotide divergence. Pairwise comparison between A. xishuangbannaensis n. sp. and the other species of Cosmocercidae with 28 S sequences available in GenBank, including Cosmocerca simile (MN 839755 – MN 839757), Cosmocercoides pulcher (LC 018444) and C. tonkinensis (AB 908160), displayed 16.78 – 17.94 % of nucleotide divergence. Partial cox 1 region Tree cox 1 sequences of A. xishuangbannaensis n. sp. (accession numbers MW 327586 – MW 327588) obtained were all 384 bp long, representing only one genotype. Tere is no species of Aplectana with cox 1 sequence registered in GenBank. Pairwise comparison between A. xishuangbannaensis n. sp. and the other species of Cosmocercidae regarding the cox 1 sequences available in GenBank, including C. japonica (LC 052756 ‒ LC 052770), C. ornata (MT 108304), Cosmocerca sp. LL- 2020 (MT 108305), C. simile (MN 833301 ‒ MN 833303), C. pulcher (MH 178306 – MH 178310, LC 052771) and C. qingtianensis (MH 178303 – MH 178305, MH 032775 – MH 032777), displayed 10.23 – 21.09 % nucleotide divergence.	en	Chen, Hui-Xia, Gu, Xiao-Hong, Ni, Xue-Feng, Li, Liang (2021): Description of a new species of AplECTana (Nematoda: Ascaridomorpha: Cosmocercidae) using an integrative approach and preliminary phylogenetic study of Cosmocercidae and related taxa. Parasites & Vectors (165) 14 (1): 1-10, DOI: 10.1186/s13071-021-04667-9, URL: http://dx.doi.org/10.1186/s13071-021-04667-9
BA5287B6FFD8FFA8FF2FFC0B8E718254.taxon	discussion	Phylogenetic analyses Phylogenetic trees inferred from maximum likelihood (ML) and Bayesian inference (BI) showed that representatives of Cosmocercoidea were divided into four major clades (Fig. 3). Clade I included the species of three genera Cosmocerca, Cosmocercoides and Aplectana, representing the family Cosmocercidae. Among the three genera, Cosmocerca displayed a closer relationship to Aplectana rather than Cosmocercoides. Clade II included only Cruzia americana (a common nematode parasite in the digestive tract of opossums), which belongs to the subfamily Cruzinae in the family Kathlaniidae according to the current classification [1]. Clade III included species of Falcaustra and Megalobatrachonema, which represent the family Kathlaniidae. Te representatives of Orientatractis and Rondonia formed Clade IV, representing the family Atractidae.	en	Chen, Hui-Xia, Gu, Xiao-Hong, Ni, Xue-Feng, Li, Liang (2021): Description of a new species of AplECTana (Nematoda: Ascaridomorpha: Cosmocercidae) using an integrative approach and preliminary phylogenetic study of Cosmocercidae and related taxa. Parasites & Vectors (165) 14 (1): 1-10, DOI: 10.1186/s13071-021-04667-9, URL: http://dx.doi.org/10.1186/s13071-021-04667-9
