3.2. 8. Triplectides puri sp. nov.

Figures 22, 23, 24

Henriques-Oliveira et al. 2020: 46 [as Triplectides misionensis Holzenthal, 1988].

Type material.

Holotype: BRAZIL • ♂; Espírito Santo, Dores do Rio Preto, Pedra Menina, Parque Nacional do Caparaó, Rio São Domingos, Cachoeira da Farofa; 20°28′19.20″S 41°49′41.90″W; alt. 1,964 m; 25 Jan. 2014; white sheet; A. L. H. Oliveira, J. L. Nessimian leg.; [DNA voucher ENT 3220]; DZRJ TRICHOPTERA 9229 . — Paratypes: BRAZIL • 1 ♀; same data as for holotype; DZRJ TRICHOPTERA 9323 • 1 larva; same data as for holotype; 27 Mar. 2012; A. LH. Oliveira leg.; [DNA voucher ENT 3231]; DZRJ TRICHOPTERA 9230 • 4 larvae; same data as for preceding; DZRJ TRICHOPTERA 9235 • 2 larvae; same data as for preceding; MNRJ • 1 ♂: Minas Gerais, Alto Caparaó, PARNA do Caparaó, Cachoeira Bonita; 20°24′4.9″S 41°50′13.3″W; alt. 1,709 m; 05 Oct. 2010; L. L Dumas, J. L. Nessimian leg.; DZRJ TRICHOPTERA 9231 • 1 ♂: Minas Gerais, Alto Caparaó, PARNA do Caparaó, 2 nd order tributary of Rio José Pedro; 20°24′35.00″S 41°50′56.70″W; alt. 1,792 m; 04 Apr. 2016; JL Nessimian, A. L. H. Oliveira, A. Antunes, A. A. Alves, J. Queiroz leg.; light trap; DZRJ TRICHOPTERA 9232 • 1 ♂: Minas Gerais, Alto Caparaó, PARNA do Caparaó, Vale Encantado, Rio José Pedro; 20°24′38.1″S 41°50′03.6W; alt. 1,912 m; 05 Oct 2010; B. Clarkson, I. C. Gonçalves leg.; MNRJ • 1 ♂: Minas Gerais, Alto Caparaó, PARNA Caparaó, Vale Verde, Rio Caparaó; 20°25′11.6″S 41°50′44.8″W; alt. 1,306 m; 05 Oct. 2010; L. L. Dumas, J. L. Nessimian leg.; DZRJ TRICHOPTERA 9234 .

Description.

Adult male. General color golden brown (in alcohol). Antennae, palps, and legs, golden brown. Wings brown to golden brown. Forewings with forks I and V present (Fig. 22 A); discoidal cell slightly enlarged apically, cross vein s almost straight, cross vein r-m short and positioned anteriorly to m-cu (Fig. 22 D) or both cross veins almost aligned (Fig. 22 C). Hind wings broad, with forks I, III, and V present (Fig. 22 B); fork I petiolate (Fig. 22 E). Length of forewing 11–12 mm, length of hind wing 8–10 mm (n = 4). Tibial spur formula 2, 2, 4. — Genitalia: Segment IX annular, in lateral view, narrow, enlarged dorsally with setose area (Fig. 23 A); in dorsal view, produced mesally and laterally, bearing setose area laterally; dorsal process absent (Fig. 23 B). Preanal appendages digitate, long, extending beyond half the length of tergum X, setose, in lateral view (Fig. 23 A). Tergum X, in lateral view, elevated at base with median area less sclerotized than apical area (Fig. 23 A); in dorsal view, wide at base, narrower at apex, apicomesal incision wide reaching less than half the length of the tergum X and forming an oval opening, apex rounded (Fig. 23 B). Inferior appendages, long, extending beyond tergum X, bearing very long setae (Fig. 23 A, C); 1 st article, in lateral view, wide at base, slightly constricted at middle-length, with apical portion narrow and apically rounded (Fig. 23 A); apicodorsal lobe digitate, long, extending beyond 2 nd article, with very long setae (Fig. 23 A, C); basoventral lobes digitate, long, extending beyond the insertion of 2 nd article, rounded and bearing long setae (Fig. 23 A, C); in ventral view, mesal lobe shorter than basoventral lobe, basal portion subrectangular, apicomesal corner produced in a digitate process, narrow and acute apically, lateral margin almost straight apically (Fig. 23 C); 2 nd article slightly widened at base, slender, curved inwards, narrowing to an acute apex (Fig. 23 C). Phallic apparatus simple, tubular, with phallotremal sclerite small, spine-like, positioned apically (Fig. 23 D, E). — Adult female. General color pale brown (in alcohol). Antennae, palps, and legs, pale brown. Length of forewing 11.0 mm, length of hind wing 9.0 mm (n = 1). Tibial spur formula 2, 2, 4. — Genitalia: Sternum VIII, in ventral view, with a subrectangular, sclerotized plate; anterior margin with a concave, and posterior margin straight (Fig. 24 A). Segment IX sclerotized dorsally, posterior margin subtruncate, and slightly rounded. Preanal appendages, in dorsal view, small, sub-oval, setose; in lateral view, subtriangular, roof-shape (Fig. 24 B), without sensilla-bearing processes (Fig. 24 A, B). Lamella oval, flap-like, and internally concave; in ventral view directed mesad (Fig. 24 B). Gonopod plate subtrapezoidal, slightly sclerotized, with apicomesal process short and slightly rugose. Spermathecal sclerite broad, bell-sharped in ventral view, and elongate in lateral view. (Fig. 24 A). — Larva. Length up to 12 mm (n = 3) (Fig. 25 A). — Head: Coloration (in alcohol) dark brown, almost homogeneous, with pale oval area around stemmata (Fig. 25 B), subrectangular, slightly flattened dorsally (Fig. 25 B). Muscle scars reddish brown. Labrum yellowish brown, sub-oval (Fig. 25 B). Mandible asymmetrical, dark, typical for Triplectides . Submentum rectangular. Ventral apotome short, subtriangular, anterior portion widened and posterior portion narrowed and truncate (resembling a champagne flute glass) (Fig. 25 B). — Thorax: Pronotum brown, with muscle scars pale; anterior margin crenulate, lateral margin slightly produced (Fig. 25 C, D). Mesonotum pale brown, almost covered by a pair of large sclerites: sa 1 each with a single seta; sa 2 each with 3 setae: (1 mesal and 1 lateral mesal long setae, and 1 posterior very short); sa 3 each with 13–17 setae. Metanotum weakly sclerotized, covered by 3 pair of sclerites: sa 1 pair subquadrate, bearing each a long seta, sa 2 pair subquadrate, with a pair of long setae, sa 3 sclerites elongate, oval, bearing each with 7–10 setae. Prosternum trapezoidal. Mesosternum with a pair of subrectangular sclerites, curved laterally (Fig. 25 E). Metasternum with a setal area bearing 7 setae (Fig. 25 E). Foretrochantin with anterodorsal margin almost straight, with corner pointed and upturned, anteroventral margin straight and corner rounded (Fig. 25 C). Legs yellowish brown, setose (Fig. 25 G). — Abdomen: Gills simple, present on segments II – VI; segments II – IV with dorsal, lateral, and ventral filaments; segment V – VI with dorsal and lateral filaments (Fig. 25 H). Segments III – VIII with lateral fringes. Segment VIII with a pair of posteromesal short setae. Segment IX dorsal sclerite with 6 long setae on posterior margin and 2 pairs of very short, lateral setae behind those, and one anterolateral short seta at each side of the sclerite (Fig. 25 F); anal claws single, large, and pointed with a very small dorsal accessory hook (Fig. 25 F). — Larval case: Length up to 20 mm. All larvae of T. puri sp. nov. were found occupying a discarded case of Marilia sp. ( Odontoceridae) (Fig. 25 I). — Pupa. Unknown.

Etymology.

The specific epithet ‘ puri ’ comes from the Coroado indigenous language, meaning audacious. The Puris are a Brazilian indigenous group belonging to the Macro-Jê linguistic branch, originally inhabiting the ES, RJ, MG, and SP States in southeast Brazil.

Distribution.

Brazil (Espírito Santo and Minas Gerais states).

Habitat.

This species was found inhabiting wide streams, with crystalline waters, and stony bottoms, with many rapids and marginal vegetation composed of Atlantic Forest or highland vegetation (campos de altitude).

Remarks.

The male of Triplectides puri sp. nov. can be confused with the one of T. misionensis Holzenthal, 1988 by the mesal lobe of the inferior appendages with a narrow apical portion (Fig. 22 A). Based on the original description of T. misionensis provided by Holzenthal (1988) and based on photographs of the holotype (USNMENT 01028388), deposited in the National Museum of Natural History (NMNH), Washington, DC, USA, we found consistent differences on this structure between these two species. In T. misionensis the mesal lobe is broad basally with a small, sclerotized basomesal point and a very narrow and divergent apical portion, but in T. puri sp. nov., the mesal lobe has the apical portion much more acute and short and the basal portion larger and subrectangular (Fig. 22 A). The larva of the new species can be distinguished from its congeners based on the following characteristics: head dark brown, almost homogeneous, subrectangular, slightly flattened dorsally (Fig. 24 B); labrum yellowish brown, subtrapezoidal, submentum rectangular and ventral apotome short, subtriangular, like a Champagne flute glass, wide at anterior portion and posterior portion truncate (Fig. 24 B). DNA sequences were obtained only for two specimens, an adult male and a larva, but they share the same haplotype for both gene fragments sequenced, thus allowing us to associate this larva.