Subfamily Hyperinae Lacordaire, 1863

Genera Brachypera Capiomont, 1868 and

Donus Jekel, 1865

The genera Brachypera, Donus and Hypera form a strongly supported monophyletic group in both our analyses (Fig. 1, Supp. 1), with both individuals of Brachypera vidua (Gené, 1837) placed within Donus samples, even if with insufficient bootstrap support (<50%).

Despite of recent efforts to unravel the relationships at genus-level based on morphology within Hyperini (Skuhrovec, 2013), werecoveredanalternativehypothesis regarding Donus and Brachypera; where the latter at best represents a subgenus within Donus . Although, in our dataset Brachypera is solely represented by Brachypera vidua. However, these results are supported by those of Stüben et al. (2015), who included Brachypera grandini (Capiomont, 1868), B. dauci (Olivier, 1807) and B. lunata Wollaston, 1854, which clustered also paraphyletically in different clades within Donus . In our analyses, the bootstrap support for two separate clades ( Donus s. l. vs Hypera) is surprisingly low and their monophyly could not be therefore definitively established based on our sequence data.