Calcarisporium guizhouense W. H. Chen, Y. F. Han & J. D. Liang sp. nov.

Fig. 3

Etymology.

Referring to its type location in Guizhou Province.

Type.

China • Guizhou Province, Qiannan Buyei and Miao Autonomous Prefecture, Duyun City, Mayao River Valley (26 ° 22 ' 8.3748 " N, 107 ° 23 ' 16.96 " E), on Cordyceps sp., 1 May 2022, Wanhao Chen, GZAC DY 0504 (holotype), ex-type DY 05041 .

Description.

Colonies on PDA attaining a diameter of 28–29 mm after 14 days at 25 ° C, white, consisting of a basal felt, floccose hyphal overgrowth, yellowish-white; reverse light brown to brown. Hyphae septate, hyaline, smooth-walled, 2.0–2.2 μm wide. Conidiophores erect, hyaline, verticillately branched, with 1–3 conidiogenous cells. Conidiogenous cells 15.6–23.2 × 1.5–1.7 μm, hyaline, cylindrical at base, gradually tapering near the apex, holoblastic to polyblastic, sympodial, apically with a cluster of conidium-bearing denticles. Conidia 5.2–8.6 × 1.8–2.2 μm, hyaline, smooth-walled, thin-walled, cylindrical, unicellular, acuminate.

Substrate.

Cordyceps sp.

Additional strain examined.

China • Guizhou Province, Qiannan Buyei and Miao Autonomous Prefecture, Duyun City, Mayao River Valley (26 ° 22 ' 8.3748 " N, 107 ° 23 ' 16.96 " E). On Cordyceps sp., 1 May 2022, Wanhao Chen, DY 05042 (living culture) .

Notes.

Calcarisporium guizhouense was easily identified as Calcarisporium, based on the BLASTn result in NCBI and its verticillate conidiophores and sympodial conidiation. Phylogenetic analyses show that Calcarisporium guizhouense has close relationships to C. arbuscula and C. yuanyangense (Fig. 1). However, C. guizhouense was easily distinguished from C. arbuscula (Conidia: ovoid to ellipsoid, 4–11 × 1.8–3.3 μm; substrate: decaying agaric) and C. yuanyangense (Conidia: ovoid to ellipsoid, 6.33–9.68 × 1.87–2.63 μm; substrate: Ophiocordyceps nutans) by its smaller cylindrical conidia and the substrate. Thus, the morphological characteristics and molecular phylogenetic results support C. guizhouense as a new species.