Conlarium sacchari L.Xie, Y.L.Chen & B.Liu sp. nov. Figure 3

Etymology.

The epithet " sacchari " refers to the habitat where first collected.

Type.

CHINA. Guangxi: Chongzuo City, Daxin County, Lanxu Village. 22°44'46"N, 107°15'15"E, 241 m alt., in sugarcane rhizosphere, 8 July 2015, Y.L. Chen and L.P. Qin, DX4 (HMAS 247299, holotype) deposited in Microbiology Research Institute, Guangxi Academy of Agricultural Science.

Description.

Colony reached 15 mm diameter on medium after 2 weeks at 28 °C, greywhite to grey, circular, flat growth, less aerial hyphae, regular edge of colony. Hyphae light yellow to yellow-brown, septate. Conidiophores yellow-brown, mostly stubby, 0-2-branched, 0-6-septate, straight or flexuous, 3 –30×2– 4 μm (10 ± 7 × 3 ± 1 μm, n = 43). Conidiogenous cells determinate, doliiform, yellow-brown to brown, 4-12 × 2-7 μm (7 ± 2 × 5 ± 1 μm, n = 52). Conidia yellow-brown to brown, muriform, irregularly globose or subglobose, smooth, constricted at the separation, 0-1 transversely septa, 0-4 longitudinal septa, 14 –19×13– 22 μm (17 ± 3 × 16 ± 2 μm, n = 20). Sexual morph: undetermined.

Habitat and distribution.

In sugarcane rhizosphere soil of southern China.

Other specimens examined.

CHINA. Guangxi: Nanning City, Long'an County, Natong Town. 23°4'48"N, 107°47'31"E, 128 m alt., in sugarcane rhizosphere, 11 Sep. 2015, Y.L. Chen and L.P. Qin, LA3 (HMAS 247300). Nanning City, Suxu town. 23°34'42"N, 108°8'30"E, 325 m alt., in sugarcane rhizosphere, 11 Feb. 2011, L. Xie, NN1 (HMAS 247301).

Notes.

Conlarium sacchari is similar to the asexual morph of C. aquaticum, C. baiseense, C. duplumascospora, C. nanningense, and C. thailandense . They all have monoblastic, holoblastic, conidiogenous cells and mostly irregular, brown, clathrate, muriform conidia (Liu et al. 2012). However, Conlarium sacchari can be easily distinguished from C. aquaticum, C. duplumascospora, C. nanningense, and C. thailandense by its less number of conidial septa (6-12-transverse septa, 4-10-longitudinal septa in C. aquaticum; 2-5 transversely septa, 0-2 longitudinal septa in C. baiseense, 2-4-transversely septate, 1-3-longitudinally septate in C. duplumascospora; 0-1 transversely septa, 0-4 longitudinal septa in C. nanningense; 4-8-transverse septa, 4-6-longitudinal septa in C. thailandense vs. 0-1 transversely septa, 0-3 longitudinal septa in C. sacchari) (Liu et al 2012; Zhang et al. 2017; Phookamsak et al. 2019). Phylogenetic reconstructions based on SSU+ITS+LSU+RBP2 sequences shows that authentic C. sacchari formed independent monophyletic groups which are well separated from C. aquaticum, C. baiseense, C. duplumascospora, C. nanningense, and C. thailandense, respectively. A comparion of ITS sequence shows that C. sacchari differ from C. aquaticum, C. baiseense, C. duplumascospora, C. nanningense, and C. thailandense in 21 bp, 24 bp, 21 bp, 18 bp, and 16 bp, resectively. Therefore, we introduce C. sacchari as a new species, following the guidelines of Jeewon and Hyde (2016).