Phylogenetic assessment and taxonomic revision of Scytalidium (Helotiales, Leotiomycetes) Author Tong, Shuo-Qiu https://orcid.org/0009-0006-9422-5551 College of Life Sciences, Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China Author Yang, Yi-Fan https://orcid.org/0009-0004-5897-8236 College of Life Sciences, Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China Author Li, Peng https://orcid.org/0009-0000-9175-6599 College of Life Sciences, Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China Author Wu, Yong-Jun 0000-0002-6732-8276 College of Life Sciences, Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China Author Sun, Bing-Da 0000-0003-0272-6422 China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China Author Zhang, Zhi-Yuan 0000-0003-2031-7518 College of Eco-Environmental Engineering, Guizhou Minzu University, Guiyang 550025, China text IMA Fungus 2025 2025-10-14 16 e 164608 e 164608 journal article 312645 10.3897/imafungus.16.164608 b4bb0c4c-775d-4eb6-9e9c-964e77874d4f PMC12541464 41132763 15. Scytalidium tongrenense S. Q. Tong & Zhi. Y. Zhang sp. nov. Fig. 5 Type. CHINA • Guizhou , Tongren , Yanhe County , Qiaojia Town , 28.28°N , 108.44°E , soil , 5 July 2023 , Shuo-Qiu Tong ( holotype HMAS 354098 , dried culture; ex-type CGMCC 3.28994 , ibid ., SQT 13) Etymology. The epithet refers to the type location. Description. Culture characteristics (14 days at 25 ° C): Colonies on PDA attaining 17–34 mm diam., flat, margin fimbriate, floral white (1 A 2) to white (1 A 1). Reverse floral white (1 A 2) to white (1 A 1). Colonies on SNA attaining 66–68 mm diam., flat, margin entire, pale gray (30 B 2). Reverse pale gray (30 B 2). Scytalidium tongrenense (from holotype HMAS 354098 ) a, b upper and reverse views of cultures on PDA and SNA after 14 d at 25 ° C c – e chlamydospore-like cells. Scale bars: 10 μm (c); 20 μm (d, e). Chlamydospore-like cells yellow-brown to dark brown, oblong to globose, ellipsoidal, subcylindrical, guttulate, or irregular, 0–1 - septate, thick-walled, smooth, catenate, 5.5–12 × 4.5–6 µm (avg. 8.1 × 5.2 μm, n = 30). Arthroconidia unknown. Sexual morph unknown. Geographical distribution. Guizhou Province , China . Additional material examined. CHINA • Guizhou , Guiyang , Huaxi District , Shiban Town , 26.46°N , 106.65°E , soil, 8 July 2022 , Shuo-Qiu Tong , SQT 14 . GenBank numbers. SQT 13 – SQT 14, ITS : PV 890028 – PV 890029 , LSU : PV 890035 – PV 890036 . Notes. Phylogenetic analysis showed that two new isolates (SQT 13 and SQT 14) clustered in a single subclade with high supported value (100 / 1) and are sister to S. album , S. assmuthi , S. aurantiacum , S. rodionovae , S. circinatum , and S. terrigenum (Figs 1 , 2 ). However, the distinctions between S. tongrenense and S. album , S. assmuthi , S. aurantiacum , S. rodionovae , S. circinatum , and S. terrigenum are provided in their respective notes. Scytalidium tongrenense was isolated from soil in a pepper cultivation area and is likely to have a saprophytic lifestyle.