Revision of Australian Tomosvaryella Aczél (Diptera: Pipunculidae) with description of 100 new species Author Motamedinia, Behnam 0000-0002-6697-4030 Canadian National Collection of Insects, Arachnids and Nematodes, Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON K 1 A 0 C 6, Canada & Centre for Biodiversity Genomics, University of Guelph, 50 Stone Road East, Guelph, Ontario, 10 N 1 G 2 W 1 Canada & bmotamed @ uoguelph. ca; bmoetamedi @ yahoo. com; https: // orcid. org / 0000 - 0002 - 6697 - 4030 * Corresponding author bmotamed@uoguelph.ca Author Földvari, Mihaly 0000-0001-6961-5255 Station Linné, Ölands Skogsby, Färjestaden, Sweden mihalyfoldvari @ gmail. com; https: // orcid. org / 0000 - 0001 - 6961 - 5255 mihalyfoldvari@gmail.com Author Skevington, Jeffrey H. 0000-0002-1445-9870 Carleton University, Biology Department, 207 Nesbitt Biology Building, 1125 Colonel By Drive, Ottawa, ON K 1 S 5 B 6, Canada jhskevington @ gmail. com; https: // orcid. org / 0000 - 0002 - 1445 - 9870 jhskevington@gmail.com Author Kelso, Scott 0000-0001-5559-9477 Canadian National Collection of Insects, Arachnids and Nematodes, Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON K 1 A 0 C 6, Canada & Canadian National Collection of Insects, Arachnids and Nematodes, Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON K 1 A 0 C 6, Canada & scott. kelso @ agr. gc. ca; https: // orcid. org / 0000 - 0001 - 5559 - 9477 scott.kelso@agr.gc.ca text Zootaxa 2023 2025-03-05 5599 1 1 271 http://dx.doi.org/10.11646/zootaxa.5360.3.8 journal article 10.11646/zootaxa.5599.1.1 1175-5334 14970961 B140A7ED-4B89-464B-8A3E-16934B175A40 Tomosvaryella uncinata Földvari, Skevington & Motamedinia sp. nov. urn:lsid:zoobank.org:act: 0BDE2B63-D736-4B5D-AEE9-37F11387ABFA Figs 100A–E , 137 , 156B Diagnosis: This species can be recognized by the hind trochanter having a small keel covered by some short bristles; slender surstyli in dorsal view ( Fig. 100A ); elongated gonopods, right gonopod with a distinct projection on inner margin; phallic guide with 5–6 dorso- dorsolateral spines; subepandrial sclerite with a central, small, fin-shaped ridge in ventral view ( Fig. 100B ); phallic guide with a hook-like projection ventrally in lateral view ( Fig. 100D ). Description: MALE: Body length: 2.6–2.8 mm . Head . Flagellum acuminate; yellow-brown. Face silvery pollinose. Frons, upper part shining black; lower part distinctly silver pollinose; eyes touching for 2 x length of ocellar triangle. Occiput silvery pollinose, less so on upper 1/3. Thorax . Postpronotum pale, with 2–3 distinct pale bristles. Mesonotum (viewed obliquely from front) silvery pollinose, anterior part more grey, greyish also from the side. Scutellum silvery brown pollinose and without bristles along distal edge. Dorsocentral bristles long, dark, mostly in frontal half, as long as ¾ width of hind femur at base, pale bristles around postpronotum absent. Halter, knob pale, stem brown. Legs . Mid coxa with 2–3 long dark bristles. Trochanters brown, femora black, shining ventrally, silvery pollinose posteriorly except shiny hind femur; knees and basal 1/4 of tibia yellow (tibia otherwise brown), tarsal segments yellow-brown dorsally, yellow ventrally, last segment darker. Hind trochanter with small keel, and 6–7 short dark bristles, equally spaced; ventrobasal spines absent on fore and mid femur. Hind femur without ventroapical spines, without long bristles posteroventrally. Subapical (distal) spines on first four tibiae well developed (longer than usual). Hind tarsomeres slightly flattened (especially hind metatarsus on distal end), and as long as 2–5 combined; posterior corners with distinct bristles on all hind tarsomeres. Pulvilli shorter than last tarsal segment. Wing . Length: 2.6–2.7 mm . Upper side of basal costal cell with one long dark brown bristle. Fourth costal section 2–2.5 times as long as third costal section. Cross-vein r-m at middle of discal cell. 2–3 short dark setulae on tegula. Abdomen . Viewed obliquely from front tergites subshiny brown-black, tergite 1 silvery grey, silvery pollinose spots on tergites 4 and 5, larger on tergite 5. Dispersed strong dark bristles on all tergites present, longest on tergite 5; length up to 1/2 the width of hind tibia at distal end. Lateral bristles on first tergite present, 4–6 dark bristles up to as long as hind femur’s width at base. Postabdomen in dorsal view; T6, S7 invisible, T5 0.6–0.8 times as long as ST8. Genitalia without dissection: ST8 large, rounded in dorsal view, brown and with distinct bristles, otherwise with velvet-like coverage; membranous area is rounded dorsally, narrow ventrally, mostly directed posteriorly; epandrium brown, surstyli yellow. Genitalia . Genital capsule in dorsal view: epandrium longer than wide (MLE:MWE = 1.6). Surstyli symmetrical, slender and elongated, curved to each other at apex, left surstylus longer than right one ( Fig. 100A ). Genital capsule in ventral view: gonopods equal in height, extended towards surstyli, pointed at apex, right gonopod with a distinct projection on inner margin; phallic guide with 5–6 dorso- dorsolateral spines, three are longer than others; subepandrial sclerite large, with a small, central, fin-shaped ridge, hypandrial apodeme distinct ( Fig. 100B ); Genital capsule in lateral view: both surstyli straight in basal three fourths and bent towards sternite apically, phallic guide with a hook-like projection ventrally before apex ( Fig. 100D–E ). Ejaculatory apodeme tube-like, bent, with a bulb in its middle ( Fig. 100C ). FEMALE: Body length: 2.2 mm . As male except for the following characters. Frons, eyes separated, as wide as 2 times the width at antennae; completely silver-grey pollinose on lower 1/3, shiny black only around ocellar triangle, smooth change in transition zone (just before ocellar triangle) to shiny black. Enlarged ommatidia silvery shining. Pulvilli and claws about 1.5–2.5 times as long as last tarsal segment, smaller on metatarsi. Female abdomen with silver spots on sides of tergites 4–6. Ovipositor. Straight in ventral view; straight, dagger-like in lateral view (yellow piercer, brown base), reaching base of abdomen; base silvery brown pollinose with numerous short dark bristles. LP:LB = 3.0. LDP:LPP = 4.0. ( Fig. 156B ). Etymology: From the Latin uncus, hook, barb or angle, in reference to the hook-like phallic guide projection. Examined material: HOLOTYPE : AUSTRALIA : Western Australia : Karijini National Park: Juna Downs Road (between drying pools in rocky Turee Creek), 22°42’S , 118°25’E , 789m , 25.IV–14.V.2003 , C. Lambkin & T. Weir, Malaise trap, CNCD3863 ( 1♂ , WAM); PARATYPES : AUSTRALIA : New South Wales : 12 miles NW Milton, 35°18’S , 150°26’E , 488m , 5.XI.1968 , D.H. Colless, JSS8671 ( 1♂ , ANIC); Culgoa National Park, 8km West-Northwest Cawwell Homestead, Diemunga Lagoon (CGN2M), 29°3’S , 146°60’E , Coolibah, 30.I–18.V.2010 , C. Lambkin, R. Olsen, B. Shieban, Malaise trap, CNC575140 ( 1♂ , QM); Porters Dam Road 16 km NW of Milton, 35°19’S , 150°26’E , 9.II.1974 , Z. Liepa, JSS9157 ( 1♂ , ANIC); Rotary Lookout 23 km NW of Milton, 35°19’S , 150°26’E , 9.II.1974 , Z. Liepa, JSS9162 ( 1♂ , ANIC); Sawpit Creek, Kosciusko National Park, 36°30’S , 148°16’E , 11.III.1978 , Z. Liepa, JSS9171 ( 1♂ , ANIC); Ledknapper National Reserve, 25.1km Northeast of Beulah Homestead, near old Gerara Springs (LDN6M), 29°16’S , 146°23’E , Mulga, 10.XII.2009 – 18.III.2010 , S. O’Sullivan & K. Taylor, Malaise trap, CNC574628 ( 1♂ , QM); Queensland : Culgoa Floodplain National Park, 7km NNW Toulby Gate (CG4AM), 28°56’S , 146°55’E , 160m , Gidgee, 20.I–19.III.2010 , C. Kelly & A. Coward, Malaise trap, CNC576381, CNC576391 ( 2♂ , QM); 17.XII.2009 – 20.I.2010 , Kelly & Coward, Malaise trap, CNC591829–30, CNC591832, CNC591835 ( 4♂ , QM); Culgoa Floodplain National Park, 7km North-Northwest of Toulby Gate (CG4AM), 28°56’S , 146°55’E , 160m , Gidgee, 19.III–20.V.2010 , C. Kelly & A. Coward, Malaise trap, CNC575358 ( 1♂ , QM); Culgoa Floodplain National Park, 7km Northwest of Toulby Gate (TOLB7M), 28°57’S , 146°53’E , 138m , reedy swamp, 20.I–19.III.2010 , C. Kelly & A. Coward, Malaise trap, CNC575364 ( 1♂ , QM); Culgoa Floodplain National Park, 7km WNW Toulby Gate (TOLB6M), 28°57’S , 146°52’E , 138m , Callitris , 19.III–20.V.2010 , C. Kelly & A. Coward, Malaise trap, CNC575019 ( 1♂ , QM); Australian Capital Territory : Nass Road, S of Tharwa, 35°31’S , 149°4’E , 28.XI.1965 , Z. Liepa, JSS9219 ( 1♂ , ANIC); Queensland : 14 km W by N Hope Vale Mission, 15°16’S , 144°59’E , 10.X.1980 , D.H. Colless, Malaise trap, JSS8564 ( 1♂ , ANIC); 2 km S of Horseshoe lookout, Blackdown land, 23°50’S , 149°3’E , 23–24.IV.1981 , D.H. Colless, JSS8615 ( 1♂ , ANIC); Culgoa Floodplain National Park, 11.8km N Headquarters (CG2AM), 28°54’S , 147°8’E , 151m , Brigalow, 20.I–18.III.2010 , C. Kelly & A. Coward, Malaise trap, CNC576673 ( 1♂ , QM); Culgoa Floodplain National Park, 7km NW Toulby Gate (TOLB7M), 28°57’S , 146°53’E , 138m , Coolibah, reedy swamp, 17.XII.2009 – 20.I.2010 , C. Kelly & A. Coward, Malaise trap, CNC575106, CNC575108–9 ( 3♂ , QM); Culgoa Floodplain National Park, 7km North-Northwest of Toulby Gate (CG4AM), 28°56’S , 146°55’E , 160m , Gidgee, 17.XII.2009 – 20.I.2010 , Kelly & Coward, Malaise trap, CNC575354–6 ( 3♂ , QM); Culgoa Floodplain National Park, 7km Northwest of Toulby Gate (TOLB7M), 28°57’S , 146°53’E , 138m , reedy swamp, 20.I–19.III.2010 , C. Kelly & A. Coward, Malaise trap, CNC575368 ( 1♂ , QM); Culgoa Floodplain National Park, 7km WNW Toulby Gate (TOLB6M), 28°57’S , 146°52’E , 138m , Callitris , 19.III–20.V.2010 , C. Kelly & A. Coward, Malaise trap, CNC575027 ( 1♂ , QM); Tasmania : 10 km NW by N St. Helens, 41°15’S , 148°10’E , 14.I.1983 , I.D. Naumann, J.C.Cardale, yellow pan trap, JSS9255, JSS9265 ( 2♂ , ANIC); Victoria : 12 km SE Merrijig, Howqua River, 37°12’S , 146°22’E , 30.XI.1971 , Neboiss, JSS8817 ( 1♂ , MVMA); 17 km SE of Merrijig, 8 mile Creek off Howqua River, 37°13’S , 146°24’E , 1.XII.1971 , Neboiss, JSS8814 ( 1♂ , MVMA); Dartmouth Survey, Eight Mile Creek, Locality JA, 37°13’S , 146°26’E , 12.IV.1973 , JSS8767 ( 1♂ , MVMA); Western Australia : 80 km S Pardoo Road House on Shay Gap Road, 20°31’S , 120°8’E , 120m , near spring with bog, 1–14.V.2003 , M.E. Irwin & F.D. Parker, Malaise trap, JSS15680 ( 1♀ , CNC); Cape Range National Park; Milyering Ranger House, 22°2’S , 113°56’E , 22m , below cliff near Ficus , 28.IV–11.V.2003 , M.E. Irwin & F.D. Parker, Malaise trap, JSS15657 ( 1♂ , CNC); Karijini National Park, Juna Downs Road, 22°42’S , 118°25’E ; 22°43’S , 118°25’E , 789m ; 799m , between drying pools in rocky Turee Creek; near rock outcrop, Eucalyptus Eremophila scrub, 14–19.V.2003 ; 25.IV–14.V.2003 , C. Lambkin & T. Weir, Malaise trap, JSS16027, JSS16061 ( 1♂ , 1♀ , ANIC); Karijini National Park: Juna Downs Road (between drying pools in rocky Turee Creek), 22°42’S , 118°25’E , 789m , 25.IV–14.V.2003 , C. Lambkin & T. Weir, Malaise trap, CNCD3817, CNCD3819, CNCD3837, CNCD3868–9 ( 1♂ , CNC; 4♂ , QM); Millstream-Chichester National Park, Snappy Gum Drive, 21°34’S , 117°4’E , 329m , across head of deep rocky gully, 27.IV–2.V.2003 , C. Lambkin & T. Weir, Malaise trap, JSS16023 ( 1♂ , ANIC); Millstream-Chichester National Park, Snappy Gum Drive, 21°34’S , 117°4’E , 329m , over deep rocky gully, 2–11.V.2003 , C. Lambkin, D. Yeates & J. Recsei, Malaise trap, JSS16181 ( 1♂ , ANIC); Newmanns Rocks, 50 km W of Balladonia Motel, 32°6’S , 123°10’E , 31.XII.1986 , G. and A. Daniels, JSS8914 ( 1♂ , QM). Distribution: Australia ( Australian Capital Territory , New South Wales , Queensland , Tasmania , Western Australia ) ( Fig. 137 ). Notes: Tomosvaryella uncinata sp. nov. is widespread in forested parts of Australia and has not been found hilltopping. Intraspecific genetic distance ranges from 0.0%. It is genetically most similar to T. cona sp. nov. (2.6– 4.8% pairwise divergence) (Supplementary file 3).