DNA barcoding, integrative taxonomy, citizen science, and Bush Blitz surveys combine to reveal 34 new species of Apanteles (Hymenoptera, Braconidae, Microgastrinae) in Australia Author Slater-Baker, Mollie-Rosae 0000-0003-0627-3117 School of Biological Sciences, The University of Adelaide, Adelaide, Australia Author Fagan-Jeffries, Erinn P. 0000-0002-3322-6255 School of Biological Sciences, The University of Adelaide, Adelaide, Australia & South Australian Museum, Adelaide, Australia Author Oestmann, Katherine J. https://orcid.org/0009-0004-3775-7127 School of Biological Sciences, The University of Adelaide, Adelaide, Australia Author Portmann, Olivia G. https://orcid.org/0009-0004-4099-3155 School of Biological Sciences, The University of Adelaide, Adelaide, Australia Author Bament, Tiahni M. https://orcid.org/0009-0009-7749-5535 School of Biological Sciences, The University of Adelaide, Adelaide, Australia Author Howe, Andy G. 0000-0002-7460-5227 Forest Research Institute, University of the Sunshine Coast, Sippy Downs, Australia Author Guzik, Michelle T. 0000-0002-4947-9353 School of Biological Sciences, The University of Adelaide, Adelaide, Australia Author Bradford, Tessa M. 0000-0003-0607-1398 School of Biological Sciences, The University of Adelaide, Adelaide, Australia & South Australian Museum, Adelaide, Australia Author McClelland, Alana R. 0000-0002-1373-1051 School of Biological Sciences, The University of Adelaide, Adelaide, Australia Author Woodward, Alice Murraylands and Riverland Landscape Board, Murray Bridge, Australia Author Clarke, Sylvia Murraylands and Riverland Landscape Board, Murray Bridge, Australia Author Ducker, Nathan Western Australian Gould League, Wembley, Australia Author Fernández-Triana, José 0000-0003-0425-0309 Canadian National Collection of Insects, Ottawa, Canada text ZooKeys 2025 2025-02-11 1227 1 128 journal article 308089 10.3897/zookeys.1227.130467 0945aff3-59f9-40c8-a95d-8a6184906808 PMC11836623 39981057 627B3463-87D6-4CA6-AAE1-B6F3CB412D75 Apanteles persephone Nixon, 1965 Fig. 6 B ( distribution), Fig. 37 B ( holotype ) Holotype information. ♀ ; Australia , WA , Yanchep , 3–19 Dec. 1935 , R. E. Turner leg. ( NHM ). Examined material. Image of the holotype and original description used to form the diagnosis. Diagnosis. Apanteles persephone is a difficult species to diagnose due to us having access to only a single image of the holotype . The species can be separated from many Apanteles in Australia by the dark metacoxa and metafemur, antenna of similar length to the body length, uniformly coloured pterostigma, fore wing veins 1 M and 1 CUa of similar pigmentation, hyaline fore wing membrane, parallel-sided and rugose T 1 , smooth T 2 , smooth mesoscutellar disc, and setae reduced to a single row on each of T 3 – T 6 . Apanteles persephone is difficult to diagnose against A. darthvaderi and A. amicalis (see key and notes under those species for more information). Notes. Whilst we collected several species that closely resembled A. persephone , we hesitate to designate any of our delimited lineages as this species without either DNA of the type, or at minimum a specimen from close to the type locality (currently not available).