Leptographium xiningense M.L. Yin, Z.W. de Beer and M.J. Wingf.

Yin, Mingliang, Wingfield, Michael J., Zhou, Xudong, Linnakoski, Riikka & Beer, Z. Wilhelm de, 2019, Taxonomy and phylogeny of the Leptographium olivaceum complex (Ophiostomatales, Ascomycota), including descriptions of six new species from China and Europe, MycoKeys 60, pp. 93-123 : 93

Leptographium xiningense M.L. Yin, Z.W. de Beer and M.J. Wingf. sp. nov. Fig. 8

Etymology.

The epithet refers to the locality where the species was first collected.

Type.

CHINA, Qinghai Province, from Picea crassifolia infested by Polygraphus poligraphus , Aug. 2010, M.L. Yin & X.D. Zhou, (PREM 60916- holotype, ex-holotype cultures CBS 136509 = CMW 38891); Qinghai Province, from Picea crassifolia infested by Polygraphus poligraphus , Aug. 2010, M.L. Yin, (PREM 60917- paratype, ex-paratype cultures CBS 136510 = CMW 39237).

Description.

Sexual state not observed. Conidiophores macronematous, synnematous, 450-550 μm including conidiogenous apparatus, synnemata occasionally slightly swollen at the base, wider at the stipe, black to brown, expanding branches at the apex, light brown to hyaline, (25 –)39–44(– 50) μm in width. Conidiogenous cells discrete, terminal, percurrent and phialidic proliferation, hyaline, cylindrical, (11 –)15–18(– 19) × 2-3 μm. Conidia hyaline, one-celled, cylindrical to obovoid, (3.9 –)4.2–4.5(– 4.8) × 1.8-2.4 μm. Cultural characteristics: Colonies on OA, spore drops hyaline at first, later becoming light to dark yellowish in the center, hyphae hyaline, appressed and immersed, synnemata predominant, aerial mycelium occasionally present on wood tissue, Optimal growth temperature on MEA is 25 °C with radial growth rate 2.0 ( ± 0.5) mm/d, growth reduced at 10 °C, no growth at 30 °C.

Host tree.

Picea crassifolia .

Insect vector.

Polygraphus poligraphus .

Distribution.

China.

Note.

This species groups closely with L. conplurium and L. erubescens , but can be distinguished by its dark conidial droplets. In addition, the synnematous conidiophores of this species were shorter, and its conidia were bigger than that of L. erubescens .

Additional material examined.

CHINA, Qinghai Province, from Picea crassifolia infested by Polygraphus poligraphus , Aug. 2010, M.L. Yin & X.D. Zhou, (culture: CMW 39238). Chongqing, from Pinus armandii infested by Dendroctonus armandi , Nov. 2018, M.L. Yin, (culture: SCAU-530). Chongqing, from Pinus armandii infested by Dendroctonus armandi , Nov. 2018, M.L. Yin, (culture: SCAU-531).

Figures

Figure 8. Leptographium xiningense sp. nov. (CMW 38891) a fourteen-days old culture on OA with black background b synnematous asexual state on wood tissue on WA c conidiophore d conidiogenous apparatus e conidiogenous cells f conidia. Scale bars: 300 μm (b), 50 μm (c), 20 μm (d), 10 μm (e), 5 μm (f).