Leptographium conplurium M.L. Yin, Z.W. de Beer & M.J. Wingf.

Yin, Mingliang, Wingfield, Michael J., Zhou, Xudong, Linnakoski, Riikka & Beer, Z. Wilhelm de, 2019, Taxonomy and phylogeny of the Leptographium olivaceum complex (Ophiostomatales, Ascomycota), including descriptions of six new species from China and Europe, MycoKeys 60, pp. 93-123 : 93

Leptographium conplurium M.L. Yin, Z.W. de Beer & M.J. Wingf. sp. nov. Fig. 4

Etymology.

The epithet refers to synnemata produced abundantly in culture.

Type.

FINLAND, Ilomantsi, from Picea abies infested with Dryocoetes autographus , Aug. 2005, Z.W. de Beer, (PREM 60918- holotype, ex-holotype cultures: CBS 128923 = CMW 23315); Ilomantsi, from P. abies infested with D. autographus , Aug. 2005, Z.W. de Bee r, (PREM 60919- paratype, ex-paratype cultures: CBS 128834 = CMW 23289).

Description.

Sexual state not observed. Conidiophores macronematous, synnematous, 300-700 μm including conidiogenous apparatus, synnemata occasionally swollen at the base, frequently swollen at the stipe, brown to black, expanding branches at the apex, (25 –)40–50(– 80) μm in width, abundantly produced in culture. Conidiogenous cells discrete, terminal, hyaline, cylindrical, (8 –)12–17(– 20) × 1.5-2.3 μm. Conidia hyaline, one-celled, ellipsoidal to cylindrical, (3.9 –)4.3–4.9(– 6.3) × 1.9-2.5 μm. Culture characteristics: colonies on OA, hyaline at first, later becoming light yellowish in the center, concentric rings present, hyphae hyaline, appressed and immersed. Optimal growth temperature is 25 °C with radial growth rate 2.5 ( ± 0.5) mm/d, growth reduced at 10 °C and 30 °C, no growth at 35 °C.

Host tree.

Picea abies .

Insect vectors.

Dryocoetes autographus , Hylastes brunneus .

Distribution.

Finland.

Notes.

All isolates of this species were initially recognized as a cryptic species closely related to L. cucullatum and L. olivaceapini by Linnakoski et al. (2012). Our results confirmed that they represent an undescribed taxon.

Additional material examined.

FINLAND, Ilomantsi, from Picea abies infested with Dryocoetes autographus , Aug. 2005, Z.W. de Beer, (culture: CMW 23295); Ilomantsi, from P. abies infested with Hylastes brunneus , Aug. 2005, Z.W. de Beer, (culture: CMW 23316).

Figures

Figure 4. Leptographium conplurium sp. nov. (CMW 23315). a fourteen-days old culture on OA with black background; b. synnematous asexual state on wood tissue on WA c conidiophore d conidiogenous apparatus e conidiogenous cells f conidia. Scale bars: 200 μm (b), 50 μm (c), 20 μm (d), 10 μm (e), 5 μm (f).