Cylindrocladiella arbusta N.Q. Pham, T.Q. Pham & M.J. Wingf.

Pham, Nam Q., Barnes, Irene, Chen, ShuaiFei, Pham, Thu Q., Lombard, Lorenzo, Crous, Pedro W. & Wingfield, Michael J., 2018, New species of Cylindrocladiella from plantation soils in South-East Asia, MycoKeys 32, pp. 1-24 : 1

Cylindrocladiella arbusta N.Q. Pham, T.Q. Pham & M.J. Wingf. sp. nov. Figure 2

Etymology.

Name refers to a plantation and the environment where this fungus was isolated.

Type material.

VIETNAM. Nghe An Province: Tan Ky, from soil in Acacia mangium plantation, Nov. 2013, N.Q. Pham & T.Q. Pham, herbarium specimen of dried culture, PREM 62159 (holotype), CMW 47295 = CBS 143546 (ex-type culture).

Description.

Sexual morph not observed. Conidiophores dimorphic, penicillate and subverticillate, mononematous and hyaline. Penicillate conidiophores comprising a stipe, a penicillate arrangement of fertile branches, a stipe extension and a terminal vesicle; stipe septate, hyaline, smooth, 116-166.5 × 4-5 µm; stipe extension aseptate, straight, 93-139 µm long, thick-walled with one basal septum, terminating in thin-walled, obpyriform to lanceolate vesicles, 4-5.5 µm wide. Penicillate conidiogenous apparatus with primary branches aseptate, 15-28.5 × 2.5-5 µm, secondary branches aseptate, 12-22.5 × 2.5-3.5 µm, each terminal branch producing 2-4 phialides; phialides doliiform to reniform to cymbiform, hyaline, aseptate, 10-18 × 2-3 µm, apex with minute periclinal thickening and collarette. Subverticillate conidiophores in moderate numbers, comprising of a septate stipe and primary branches terminating in 2-4 phialides; primary branches straight, hyaline, 0-1-septate, 25-31 × 2.5-3.5 µm; phialides cymbiform to cylindrical, hyaline, aseptate, 16.5-30.5 × 2-3.5 µm, apex with minute periclinal thickening and collarette. Conidia cylindrical, rounded at both ends, straight, 1-septate, (8.5 –)10–12(– 13.5) × 2-3 µm (av. = 11 × 2.5 µm), frequently slightly flattened at the base, held in asymmetrical clusters by colourless slime.

Culture characteristics.

Colonies white to buff on the surface and salmon to sienna in reverse on MEA after 7 d; smooth margins; extensive aerial mycelium in the middle and the margins; chlamydospores moderate, arranged in chains. Optimal growth temperature at 25 °C, no growth at 5 °C and 35 °C; after 7 d, colonies at 10 °C, 15 °C, 20 °C, 25 °C and 30 °C reached 3.5 mm, 27.7 mm, 49.2 mm, 67.9 mm and 52.7 mm, respectively.

Additional material examined.

VIETNAM, Nghe An Province: Tan Ky, from soil in Acacia mangium nursery, Nov. 2013, N.Q. Pham & T.Q. Pham, PREM 62160, culture CMW 47296 = CBS 143547.

Distribution.

Nghe An, Vietnam.

Notes.

Cylindrocladiella arbusta is phylogenetically closely related to Cy. natalensis , Cy. obpyriformis and Cy. parvispora . The stipe extensions of Cy. arbusta are longer than those of Cy. natalensis and shorter than those of Cy. obpyriformis and Cy. parvispora . Conidia of Cy. arbusta are shorter than those of Cy. natalensis and Cy. obpyriformis (Table 2).